Method for preparing nano hydroxylapatite hybridized material with surface grafting polypeptide

Abstract

The invention provides a nano-hydroxyl apatite hybrid material which is grafted with peptide on the surface and a method for making the same. First of all, the nano- hydroxyl apatite is treated with a silane coupling agent gamma-amino propyl triethoxy which contains amido, the amido is introduced in the surface of the nano-hydroxy apatite; then, gamma-benzyl-L-glutamate N- carboxyanhydride ring-opening polymerization are caused by using the nano- hydroxyl apatite hybrid material which containing the amido on the surface, so as to get the nano- hydroxyl apatite hybrid material which is grafted with polypeptide on the surface. This method is universally used, by adopting the nano-hydroxy apatite with amido to evoke N-carboxyanhydride of other amino acid, the peptides with different functions are easily to be connected to the surface of hydroxyl apatite through chemical bond to give the hydroxyl apatite new biological properties, therefore having a very good application value.

Description

technical field

[0001] The invention relates to a method for preparing a surface-grafted polypeptide nano-hydroxyapatite hybrid material technical background

[0002] Hydroxyapatite has excellent biocompatibility and osteoconductivity, and is the main component of vertebrate bones and teeth. For example, the mass percentage of hydroxyapatite in human bone is close to 60%, and the enamel surface of human teeth The mass fraction of gray stone accounts for more than 90%. Therefore, hydroxyapatite is an ideal bone tissue replacement material, but hydroxyapatite has high brittleness, insufficient toughness, and poor mechanical properties. In recent years, hydroxyapatite / polymer composites have effectively combined the osteoconductivity of hydroxyapatite with the toughness and biodegradability of polymers, and have become a hot spot in bone repair materials (Biomaterials, 12, 841- 847. Biomaterials, 15, 1197-1200. Composites Science and Technology, 65, 2385-2406.). The mechanic...