Method for preparing nano hydroxylapatite hybridized material with surface grafting polypeptide

A nano-hydroxyapatite, surface grafting technology, applied in medical science, prosthesis and other directions, can solve problems such as no reports

Inactive Publication Date: 2008-11-12
CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Through silane coupling agent (J.Biomed.Mater.Res., 30,231-238.), polylactic acid (Polymer, 45,6699-6706.), isocyanate (J.Biomed.Mater.Res., 40, 358-364.) etc. have reported the surface treatment of hydroxyapatite to prepare hybrid materials, but there is no report on the preparation of surface-grafted polypeptide hydroxyapatite hybrid materials by ring-opening polymerization.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0010] 1) First dissolve 2.21g of γ-aminopropyl-triethoxysilane in 100mL of ethanol / water mixed solvent with a volume ratio of 90:10 and stir for 30 minutes, then add 6.63g of nano-hydroxyapatite and react for 3 hours, The pH value of the reaction system was adjusted to 9-10 with ammonia water, and the stirring was continued for 3 hours. Centrifuge, collect the solid matter, and dry it in an oven at 130°C for 2 hours, then wash it with ethanol for 3 times, and dry it to obtain nano-hydroxyapatite with amino groups on the surface. The surface γ-aminopropyl The amount of grafted base-triethoxysilane is 2.5%.

[0011] 2) Under argon protection, add 0.2 g of nano-hydroxyapatite containing amino groups on the surface to the reaction flask, and then add 20 mL of dioxane with a syringe. Stir for 5 minutes, and then sonicate for 30 minutes to fully disperse the nano-hydroxyapatite containing amino groups on the surface.

[0012] 3) Add 0.2g of γ-benzyl-L-glutamic acid-N-carbonyl anh...

Embodiment 2

[0015] 1) First, dissolve 2.21g of γ-aminopropyl-triethoxysilane in 100mL of ethanol / water mixed solvent with a volume ratio of 95:5 and stir for 30 minutes, then add 6g of nano-hydroxyapatite and react for 3 hours, Then adjust the pH value of the reaction system to 9-10 with sodium hydroxide, and continue to stir for 3 hours. Centrifuge to collect the solid matter, and dry it at 130°C for 2 hours, then wash it with ethanol for 3 times, and dry it to obtain nano-hydroxyapatite with amino groups on the surface. - The amount of triethoxysilane grafted is 3.1%.

[0016] 2) Operation 2) in "Example 1"

[0017] 3) Operation 3) in "Example 1"

[0018] 4) Add the γ-benzyl-L-glutamic acid-N-carbonyl internal anhydride prepared in 3) into the nano-hydroxyapatite turbid solution containing amino groups on the surface with a syringe, and react at room temperature for 48 hours. Centrifuge to collect the solid product, wash the product three times with dioxane, and dry to obtain the nan...

Embodiment 3

[0020] 1) The operation is the same as 1) in "Example 1".

[0021] 2) The operation is the same as 2) in "Example 1".

[0022] 3) The operation is the same as 3) in "Example 1".

[0023] 4) Use a syringe to add the γ-benzyl-L-glutamic acid-N-carbonyl ring anhydride solution prepared in 3) to the nano-hydroxyapatite turbid solution containing amino groups on the surface prepared in 2), and react at room temperature for 72 Hour. Centrifuge to collect the solid product, wash the product three times with dioxane, and dry it in vacuum to obtain a nano-hydroxyapatite hybrid material grafted on the surface. Its graft rate is 33.5%.

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Abstract

The invention provides a nano-hydroxyl apatite hybrid material which is grafted with peptide on the surface and a method for making the same. First of all, the nano- hydroxyl apatite is treated with a silane coupling agent gamma-amino propyl triethoxy which contains amido, the amido is introduced in the surface of the nano-hydroxy apatite; then, gamma-benzyl-L-glutamate N- carboxyanhydride ring-opening polymerization are caused by using the nano- hydroxyl apatite hybrid material which containing the amido on the surface, so as to get the nano- hydroxyl apatite hybrid material which is grafted with polypeptide on the surface. This method is universally used, by adopting the nano-hydroxy apatite with amido to evoke N-carboxyanhydride of other amino acid, the peptides with different functions are easily to be connected to the surface of hydroxyl apatite through chemical bond to give the hydroxyl apatite new biological properties, therefore having a very good application value.

Description

technical field [0001] The invention relates to a method for preparing a surface-grafted polypeptide nano-hydroxyapatite hybrid material technical background [0002] Hydroxyapatite has excellent biocompatibility and osteoconductivity, and is the main component of vertebrate bones and teeth. For example, the mass percentage of hydroxyapatite in human bone is close to 60%, and the enamel surface of human teeth The mass fraction of gray stone accounts for more than 90%. Therefore, hydroxyapatite is an ideal bone tissue replacement material, but hydroxyapatite has high brittleness, insufficient toughness, and poor mechanical properties. In recent years, hydroxyapatite / polymer composites have effectively combined the osteoconductivity of hydroxyapatite with the toughness and biodegradability of polymers, and have become a hot spot in bone repair materials (Biomaterials, 12, 841- 847. Biomaterials, 15, 1197-1200. Composites Science and Technology, 65, 2385-2406.). The mechanic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/12A61L27/22
Inventor 陈学思魏俊超刘爱学庄秀丽景遐斌
Owner CHANGCHUN INST OF APPLIED CHEMISTRY - CHINESE ACAD OF SCI
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