Eureka AIR delivers breakthrough ideas for toughest innovation challenges, trusted by R&D personnel around the world.

Serum-free culture medium suitable for immune cell large-scale culture

A serum-free medium, large-scale culture technology, applied in the field of cell biology and medical immunology, can solve the problems of difficult to meet the reinfusion standard, long culture time, etc., to avoid safety problems, reduce production costs, and increase proliferation effect. Good results

Active Publication Date: 2015-11-11
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
View PDF3 Cites 12 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, the most widely used serum-free medium is LONZA’s XVIVO serum-free medium and GIBCO’s AIM serum-free medium, but the above two mediums need to be supplemented with autologous plasma in the early stage of cell culture, otherwise, when culturing immune cells, It is difficult to meet the clinical reinfusion standard, and the culture time is long

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Serum-free culture medium suitable for immune cell large-scale culture
  • Serum-free culture medium suitable for immune cell large-scale culture
  • Serum-free culture medium suitable for immune cell large-scale culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] A serum-free medium suitable for large-scale cultivation of immune cells, based on RPMI1640 medium, also contains additional components, the concentration of which is: hydroxyethylpiperazine sulfuric acid 3 / L, phenol red sodium 0.0001g / L, L-glutamate 0.1g / L, sodium bicarbonate 0.5g / L. The added ingredients also include 0.001g / L of insulin, 0.001g / L of transferrin, 0.0001g / L of selenium, 2g / L of human albumin, and 240IU / mL of interleukin.

[0043] The RPMI1640 medium includes inorganic salts, amino acids, vitamins, and sugars.

[0044] The concentration of the inorganic salt is: calcium dinitrate tetrahydrate 0.01g / L, magnesium sulfate anhydrous 0.04g / L, potassium chloride 0.04g / L, sodium bicarbonate 0.2g / L, sodium chloride 2g / L, Disodium hydrogen phosphate 0.08g / L; The concentration of the amino acid is: L-arginine

[0045] 0.02g / L, L-valine 0.002g / L, L-aspartic acid 0.002g / L, L-asparagine 0.005g / L, L-cysteine ​​dihydrochloride 0.006g / L, L-glutamic acid 0.002g / L, L-g...

Embodiment 2

[0051] A serum-free medium suitable for large-scale cultivation of immune cells, based on RPMI1640 medium, also contains additional components, the concentration of which is: hydroxyethylpiperazine sulfuric acid 9 / L, phenol red sodium 0.1g / L, L-glutamate 1g / L, sodium bicarbonate 8g / L. The added ingredients also include insulin 0.1g / L, transferrin 0.01g / L, selenium 0.1g / L, human albumin 60g / L, interleukin-2600IU / mL.

[0052] The RPMI1640 medium includes inorganic salts, amino acids, vitamins, and sugars.

[0053] The concentration of the inorganic salt is: calcium dinitrate tetrahydrate 1g / L, magnesium sulfate anhydrous 0.4g / L, potassium chloride 2g / L, sodium bicarbonate 8g / L, sodium chloride 10g / L, dihydrogen phosphate Sodium 4g / L; the concentration of the amino acid is: L-arginine 2g / L, L-valine 2g / L, L-aspartic acid 2g / L, L-asparagine 0.5g / L, L-cysteine·dihydrochloride 0.6g / L, L-glutamic acid 0.2g / L, L-glutamine 0.3g / L, L-histidine 0.15g / L, glycine 0.1g / L, L-isoleucine 0....

Embodiment 3

[0059] A serum-free medium suitable for large-scale cultivation of immune cells, based on RPMI1640 medium, also contains additional components, the concentration of which is: hydroxyethylpiperazine sulfuric acid 5g / L, phenol red sodium 0.002g / L, L-glutamate 0.2g / L, sodium bicarbonate 1g / L, insulin 0.005g / L, transferrin 0.002g / L, selenium 0.001g / L, human albumin 5g / L , Interleukin-250IU / mL.

[0060] The RPMI1640 medium includes inorganic salts, amino acids, vitamins, and sugars.

[0061] The concentration of the inorganic salt is: calcium dinitrate tetrahydrate 0.1g / L, magnesium sulfate anhydrous 0.04884g / L, potassium chloride 0.4g / L, sodium bicarbonate 2g / L, sodium chloride 6g / L, phosphoric acid Disodium hydrogen 0.8g / L; The concentration of the amino acid is: L-arginine 0.2g / L, L-valine 0.02g / L, L-aspartic acid 0.02g / L, L-aspartic acid Amide 0.05g / L, L-cysteine·dihydrochloride 0.0652g / L, L-glutamic acid 0.02g / L, L-glutamine 0.3g / L, L-histidine 0.015g / L, Glycine 0.01g / L, L-...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a serum-free culture medium suitable for immune cell large-scale culture, and belongs to the field of cell biology and medical immunology. The serum-free culture medium takes an RPMI1640 culture medium as a basis, additive components are further contained and include HEPES, phenol red sodium salt, L-glutamate and sodium bicarbonate. Insulin, transferrin, selenium, human albumin and interleukin-2 can be further added. Components contained in the culture medium are chemical defined components, no animal serum components are contained, and the serum-free culture medium is suitable for large-scale culture of immunity cells and is high in clinical safety and good in proliferation effect.

Description

technical field [0001] The invention belongs to the fields of cell biology and medical immunology, and in particular relates to a serum-free medium suitable for culturing all immune cells. Background technique [0002] With the rapid development of immune cell biology and immune molecular biology, somatic cell immunotherapy has become one of the important means of adjuvant therapy for tumor patients after radiotherapy and chemotherapy. All have good effect. [0003] Somatic cell immunotherapy is a biological therapy that injects immune cells with anti-tumor activity into tumor patients to directly kill the tumor or stimulate the body's anti-tumor immune response to achieve therapeutic effects. In vitro manipulations include cell passage, expansion, modification, screening and treatment with drugs or other treatments that can change the biological behavior of cells in vitro. Somatic cells manipulated in vitro can be used for tumor treatment and also for tumor prevention. ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N5/0783C12N5/0784
Inventor 葛啸虎陈海佳王一飞罗二梅王小燕
Owner GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com