Method for obtaining action mechanism of nanometer probe and single cell

Abstract

The invention discloses a method for obtaining an action mechanism of a nanometer probe and a single cell, which relates to the fields of atomic force microscope and cytomechanics. A cell membrane piercing force (F1) and a cell nuclear envelope piercing force (F2) are determined to being able to reflecting external forces that cells can bear by curves and thus the external force values that are needed at least for piercing the cell membrane and the cell nuclear envelope can be reflected in a quantitative mode. The piercing force hopping (Fd1,k1) of the cell membrane and the hopping (Fd2,k2) of the cell nuclear envelope piercing force can reflect differences between external and internal materials of the cell membrane and external and internal materials of the cell nuclear envelope; the larger the Fd and k values, the larger the characteristic differences of the external and internal materials of the cell membrane and cell nuclear envelope; and when the differences are larger than certain values, piercing of the needle point into the cell membrane or the cell nuclear envelope can be determined. On the basis of statistics of lots of data, the Fd is set to be larger than 0.07Nn and the k is set to be larger than 0.5Nn / microns; and because of the quantitative statistics, the determination data can be used as piercing data for determining a cell membrane and a cell nuclear envelope intelligently in future.

Description

technical field [0001] The invention relates to the fields of atomic force microscope and cell mechanics. The nanoprobe of atomic force microscope (AFM) is used to physically penetrate single cells, and a 10-parameter method for systematically analyzing the action mechanism of nanoprobes and single cells is invented. This is of great significance for the comprehensive understanding of cell mechanics and cell nuclear mechanics. Background technique [0002] The study of single-cell mechanics based on probe technology is a hot research topic at present. The elastic modulus of cells, the adhesion between cells, and the adhesion between cells and extracellular matrix can be measured by the probe acting on a single cell. Recently, the study of the mechanical properties of living cell nuclei using nanoprobes based on atomic force microscopy has become a hot topic of concern for relevant scholars. In the process of measuring the mechanical properties of the nucleus, the nanoprobe...

AI Technical Summary

Problems solved by technology

However, at present, the analysis of force curves of nanoprobes penetrating cell membrane and nucleus only focuses on measuring the elastic modulus of cell membrane and nucleus, ignoring other important data of probe penetrating cell membrane and nucleus, such as probe penetrating cell membrane and The force to be exerted by the nucleus, the penetration depth of the probe through the cell membrane and the intrusion depth of the nucleus, etc. These data are critical to a comprehensive understanding of the interaction between the probe and the living cell

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