Method for obtaining action mechanism of nanometer probe and single cell

A Nanoprobe, Mechanism of Action Technology, Applied in Atomic Force Microscopy and Cell Mechanics

Inactive Publication Date: 2017-06-20
UNIV OF ELECTRONICS SCI & TECH OF CHINA
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, at present, the analysis of force curves of nanoprobes penetrating cell membrane and nucleus only focuses on measuring the elastic modulus of cell membrane and nucleus, ignoring other important data of probe penetrating cell membrane and nucleus, such as probe penetratin

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  • Method for obtaining action mechanism of nanometer probe and single cell
  • Method for obtaining action mechanism of nanometer probe and single cell
  • Method for obtaining action mechanism of nanometer probe and single cell

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Experimental program
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Embodiment Construction

[0029] 1. Culture method of human skin fibroblasts (Fibroblasts referred to as "FB") and breast cancer cells (MCF-7)

[0030] FB and MCF-7 were each cultured in a 35mm diameter petri dish, the culture medium was DMEM / HIGH GIUCOSE and 10% fetal bovine serum (FBS), and the cells were cultured at 37°C, 5% CO 2 incubator for at least 24 hours. figure 1 Topography of individual fibroblasts obtained for human skin fibroblasts in a Petri dish and atomic force microscopy. figure 2 Topography of a single breast cancer cell obtained for breast cancer cells in a petri dish and atomic force microscopy.

[0031] 2. AFM experiment and parameter design

[0032] In order to illustrate the universality of this method, two kinds of cells, fibroblasts ( figure 1 ) and breast cancer cells ( figure 2 ); two shapes of probes were selected, the commercial pyramid-shaped silicon nitride tip ( image 3 (a)) and focused ion beam (FIB) processed SiN nano-needles ( image 3 (b)). Three sets of e...

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Abstract

The invention discloses a method for obtaining an action mechanism of a nanometer probe and a single cell, which relates to the fields of atomic force microscope and cytomechanics. A cell membrane piercing force (F1) and a cell nuclear envelope piercing force (F2) are determined to being able to reflecting external forces that cells can bear by curves and thus the external force values that are needed at least for piercing the cell membrane and the cell nuclear envelope can be reflected in a quantitative mode. The piercing force hopping (Fd1,k1) of the cell membrane and the hopping (Fd2,k2) of the cell nuclear envelope piercing force can reflect differences between external and internal materials of the cell membrane and external and internal materials of the cell nuclear envelope; the larger the Fd and k values, the larger the characteristic differences of the external and internal materials of the cell membrane and cell nuclear envelope; and when the differences are larger than certain values, piercing of the needle point into the cell membrane or the cell nuclear envelope can be determined. On the basis of statistics of lots of data, the Fd is set to be larger than 0.07Nn and the k is set to be larger than 0.5Nn/microns; and because of the quantitative statistics, the determination data can be used as piercing data for determining a cell membrane and a cell nuclear envelope intelligently in future.

Description

technical field [0001] The invention relates to the fields of atomic force microscope and cell mechanics. The nanoprobe of atomic force microscope (AFM) is used to physically penetrate single cells, and a 10-parameter method for systematically analyzing the action mechanism of nanoprobes and single cells is invented. This is of great significance for the comprehensive understanding of cell mechanics and cell nuclear mechanics. Background technique [0002] The study of single-cell mechanics based on probe technology is a hot research topic at present. The elastic modulus of cells, the adhesion between cells, and the adhesion between cells and extracellular matrix can be measured by the probe acting on a single cell. Recently, the study of the mechanical properties of living cell nuclei using nanoprobes based on atomic force microscopy has become a hot topic of concern for relevant scholars. In the process of measuring the mechanical properties of the nucleus, the nanoprobe...

Claims

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Application Information

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IPC IPC(8): G01Q60/24
CPCG01Q60/24
Inventor 范娜彭倍姜海王贵学叶志易张国成杨龙祥王群
Owner UNIV OF ELECTRONICS SCI & TECH OF CHINA
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