1 results about "Selective cleavage" patented technology

This specification discloses a method for high-throughput evaluation of the in vitro cleavage activity of a CRISPR / Cas system. The inventors of this application have implemented this method based on the concept that an in vitro environment can be simulated by immobilizing cells to inhibit their viability, while partially utilizing existing established high-throughput evaluation methods for in vivo environmental activity. Furthermore, this specification discloses a model for predicting the cleavage activity of a CRISPR / Cas system and its application method. This includes: 1) collecting cleavage activity data using various combinations of target-guide vectors; 2) obtaining two or more alignment patterns to augment the data when the target nucleic acid and guide nucleic acid do not match; and 3) inventing the model by developing a method for training the model using aligned target nucleic acid sequences and aligned guide nucleic acid sequences as key input variables, and applying the model in various ways. Additionally, this specification discloses a method for enriching rare nucleic acids using a CRISPR / Cas system when a sample contains trace amounts of rare nucleic acids and a large amount of background nucleic acids. The inventors selected a guide nucleic acid (optimized guide nucleic acid) that promotes selective cleavage of the nucleic acid to be cleaved (rare nucleic acid or background nucleic acid) relative to nucleic acids with similar sequences (background nucleic acids or rare nucleic acids), and used it to develop a rare nucleic acid enrichment method.