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Method and device for continuously removing cell/bacterial lysis floccules

A technology for flocs and cells, which is applied in the field of continuous removal of cell/bacteria lysing flocs and devices, can solve the problems of clogging of sieve holes, reduction of production efficiency and product quality, low efficiency, etc., and achieves splash prevention and convenient operation. , the effect of ensuring safety

Inactive Publication Date: 2011-11-16
格兰柏生化科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Different experiments have also proved that large bag filters can filter out flocs, but their efficiency is not only very low, but also the mesh holes of large bag filters are easily blocked by viscous flocs, which also causes Consequences of reduced productivity and product quality
Increasing the pressure at the inlet of the large bag filter will not improve the filtration efficiency but will also increase the back pressure and shear force, thereby destroying the structure of the supercoiled DNA

Method used

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  • Method and device for continuously removing cell/bacterial lysis floccules
  • Method and device for continuously removing cell/bacterial lysis floccules
  • Method and device for continuously removing cell/bacterial lysis floccules

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Embodiment Construction

[0019] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments.

[0020] Such as figure 1 , figure 2 As shown, the device of the present invention includes a frame 1, the bottom of the frame 1 is provided with a motor 2, and the speed of the motor 2 can be adjusted by an electronic frequency regulator or other speed control devices in the prior art. The output end of the motor 2 drives two transmission shafts 4 supported horizontally on the frame 1 to rotate in the same direction through the transmission mechanism 3 , and two pairs of friction rollers 5 are correspondingly arranged on the two transmission shafts 4 . A drum 6 is arranged on the upper part of the frame 1. The drum 6 includes two driving rings 7 corresponding to the positions of the two pairs of friction rollers 5. Between the two driving rings 7, a plurality of transverse connecting rods are arranged at intervals along the circumference of the drum ...

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Abstract

The invention relates to a method and device for continuously removing cell / bacterial lysis floccules. The method comprises the following steps of: 1) arranging a solid-liquid separation device, wherein the solid-liquid separation device comprises a motor and a roller driven by a transmission mechanism, the roller is internally provided with blades extending forwards in a spiral shape, a screen is arranged outside the roller, a collection bed is arranged below the roller, and the liquid outlet of the collection bed is connected with a downstream deep bed filter by virtue of a liquid pump; 2) starting a motor to drive the roller to rotate, feeding a lysis solution containing solid floccules into the roller while the lysis solution spirally advances under the driving of the blades, and carrying out solid-liquid separation; and 3) causing a liquid to flow into the collection bed by virtue of the screen, and taking the solid floccules out of the roller by virtue of the blades. In the invention, the lysis solution is advanced under the actions of the blades and the screen while the solid-liquid separation is carried out, the liquid flows into the collection bed by virtue of the roller, and dehydrated solid floccules can be discharged from a solid collection hole, thus the method and device provided by the invention can be widely applied to a production process for continuously removing cell / bacterial lysis floccules in a large scale.

Description

technical field [0001] The invention relates to a solid-liquid separation method and device, in particular to a method and device for continuously removing cell / bacteria lysed flocs. Background technique [0002] The approval of the first plasmid DNA to protect equids against West Nile virus in 2007 suggests that plasmid DNA vaccines are also an alternative to conventional vaccines. Due to the huge number of poultry and domestic animals, a considerable amount of plasmid DNA is required to satisfy veterinary vaccines and therapeutic drugs. In the large-scale plasmid DNA production process, one hundred kilograms of solid flocs will be produced after lysing cells / bacteria from one-hundred-liter fermentors, and the fragile flocs produced after cell / alkaline lysis will be removed Always been a big problem. In the laboratory, a small amount of flocs can be separated by high-speed centrifugation or cheese cloth, but laboratory techniques are difficult to use in large-scale produc...

Claims

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Application Information

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IPC IPC(8): B01D33/11B01D33/64B01D33/48B01D33/74
Inventor 卫星辉周子龙
Owner 格兰柏生化科技有限公司
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