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Method for quickly preparing polyethylene glycol regulated and controlled nano hydroxyapatite

A technology of nano-hydroxyapatite and polyethylene glycol, which is applied in the field of biomedical materials, can solve the problems of good dispersion and inability to prepare sizes, and achieve good dispersion, mild and controllable preparation conditions, and simple preparation methods Effect

Inactive Publication Date: 2015-04-22
ZHEJIANG SCI-TECH UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, various experimental methods cannot prepare hydroxyapatite nanoparticles with small size and good dispersibility, so it is urgent to develop a hydroxyapatite nanoparticle with simple preparation conditions, short time consumption, low energy consumption, small particle size and high dispersibility. Good preparation method of nano-hydroxyapatite

Method used

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  • Method for quickly preparing polyethylene glycol regulated and controlled nano hydroxyapatite
  • Method for quickly preparing polyethylene glycol regulated and controlled nano hydroxyapatite
  • Method for quickly preparing polyethylene glycol regulated and controlled nano hydroxyapatite

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Prepare 15mM calcium nitrate solution and 9mM diammonium hydrogen phosphate solution respectively. First, cool 50mL of calcium nitrate solution at 0°C, and then add 50mL of diammonium hydrogen phosphate solution into the calcium nitrate solution at 10 drops / min through a constant flow pump. During the dropwise addition, the stirring speed was gradually increased to 500 rpm / min at a rate of 10 rpm / min, and the pH of the reaction system was maintained at 9 with 1M sodium hydroxide solution during the reaction. After the dropwise addition was completed, 0.5 mg of polyethylene glycol-200 was added dropwise. After the dropwise addition was completed, the stirring was stopped and left to stand for 1 hour. The system was rapidly stirred at 500 rpm / min, and the temperature was raised to 70° C., and the stirring was continued for 1 h. Stop heating, and after the reaction system cools down to room temperature, centrifuge and resuspend with deionized water at 4°C at low temperatu...

Embodiment 2

[0032] Prepare 50mM calcium chloride solution and 30mM disodium hydrogen phosphate solution respectively. First, put 50mL of calcium chloride solution at 0°C to cool, and then add 200mL of disodium hydrogen phosphate solution into the calcium chloride solution at 60 drops / min through a constant flow pump. During the dropwise addition, the stirring speed was gradually increased to 1000 rpm / min at a rate of 20 rpm / min, and the pH of the reaction system was maintained at 14 with 1M sodium hydroxide solution during the reaction. After the dropwise addition was completed, 10 mg of polyethylene glycol-2000 was added dropwise. After the dropwise addition was completed, the stirring was stopped and left to stand for 1 hour. The system was rapidly stirred at 1000 rpm / min, and the temperature was raised to 100° C., and the stirring was continued for 1 h. Stop heating, and after the reaction system cools down to room temperature, centrifuge and resuspend with deionized water at a low te...

Embodiment 3

[0034]Prepare 40mM calcium chloride solution and 24mM diammonium hydrogen phosphate solution respectively. First, place 40mL of calcium chloride solution at 0°C to cool, and then add 160mL of diammonium hydrogen phosphate solution into the calcium chloride solution at 40 drops / min through a constant flow pump. During the dropwise addition, the stirring speed was gradually increased to 800 rpm / min at a rate of 15 rpm / min, and the pH of the reaction system was maintained at 10 with 1M sodium hydroxide solution during the reaction. After the dropwise addition is completed, add polyethylene glycol-400 6mg dropwise. After the dropwise addition is completed, stop stirring and let stand for 1h. The system was rapidly stirred at 800 rpm / min, and the temperature was raised to 90° C., and the stirring was continued for 1 h. Stop heating, and after the reaction system cools down to room temperature, centrifuge and resuspend with deionized water at a low temperature of 4°C, and wash the ...

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Abstract

The invention discloses a method for quickly preparing polyethylene glycol regulated and controlled nano hydroxyapatite and belongs to the field of preparation of biomedical materials. The method comprises the following process steps: preparing a calcium salt solution and a phosphate solution respectively, and mixing the calcium salt solution and the phosphate solution with equal volumes; adjusting the pH value of a reaction system by using ammonia water while stirring; adding polyethylene glycol, and heating the reaction system; and performing centrifuging and washing after reaction, and collecting granular deposits to obtain nano hydroxyapatite granular powder. The method disclosed by the invention is simple in preparation process, strong in laboratory operability, low in production cost and high in product yield and is suitable for laboratory research application and industrial production; and moreover, the prepared rod-shaped nano hydroxyapatite has good dispersity, does not have toxic reaction to normal cells and cancer cells, and has a huge application potential in the fields of biomedicine and biological materials such as drug controlled-release vectors, gene therapy vectors and the like.

Description

technical field [0001] The invention belongs to the technical field of biomedical materials, and in particular relates to a rapid preparation method of nano-hydroxyapatite regulated by polyethylene glycol. Background technique [0002] Hydroxyapatite nanoparticles have extensive and important uses in the fields of coatings, medicine, and pharmacy, and can be used as a nano-inorganic material with good histocompatibility and no toxic side effects for gene or drug delivery and mitigation. release carrier. However, during the preparation process, hydroxyapatite will expand in size due to continuous growth, and cannot carry out effective intracellular transport. Therefore, how to easily prepare nano-sized and well-dispersed hydroxyapatite has become the focus of current research. In the existing preparation methods, it has been reported that nano-hydroxyapatite with different structures and shapes can be produced, such as Klesing J, Chernousova S and Epple M. Freeze-dried cati...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C01B25/32
Inventor 孔祥东李泽豪叶婷江国华李麟涉姚菊明
Owner ZHEJIANG SCI-TECH UNIV
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