Preparation method and new application of polyinosinic preparation

A technology of polymyocyte complex and new use, applied in the field of anti-tumor effect, can solve problems such as loss of effect

Inactive Publication Date: 2012-06-13
TIANJIN JIMINGSHENG BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, polysarcoma is susceptible to degradation by RNases in the body and loses its effect

Method used

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  • Preparation method and new application of polyinosinic preparation
  • Preparation method and new application of polyinosinic preparation

Examples

Experimental program
Comparison scheme
Effect test

example 1

[0016] Inhibitory effect of example 1 polymyocyte complex on mouse sarcoma (S180)

[0017] (1) Experimental method:

[0018] Mouse sarcoma (S180) in vitro cell line was resuscitated with liquid nitrogen, subcultured for several times, digested and centrifuged, and washed with 10 7 The concentration of cells per ml (0.2ml) was inoculated subcutaneously in healthy KM mice. After 15 days, a solid tumor with a size of one centimeter was formed. The nude mice were killed by neck dislocation, soaked in 70% alcohol for 1 minute, and the tumor was peeled off under sterile conditions and cut into 1mm 3 The size of the tissue block, select fresh tumor tissue without necrosis, wash five times with normal saline, gently grind to a cell suspension with a tissue homogenizer, filter with a 150-mesh cell sieve, count viable tumor cells, prepare tumor cell suspension, and The concentration is 1×10 7 / ml, subcutaneously inoculate 0.1ml (1×10 6 / ml).

[0019] (2) Experimental results:

[00...

example 2

[0021] Inhibition of Example 2 Polymyocyte Complex to Mouse Liver Cancer (H22) Cells

[0022] (1) Experimental method:

[0023] Mouse liver cancer (H22) in vitro cell line was resuscitated with liquid nitrogen, subcultured for several times, digested and centrifuged, and washed with 10 7 The concentration of cells per ml (0.2ml) was inoculated subcutaneously in healthy KM mice. After 15 days, a solid tumor with a size of one centimeter was formed. The nude mice were killed by neck dislocation, soaked in 70% alcohol for 1 minute, and the tumor was peeled off under sterile conditions and cut into 1mm 3 The size of the tissue block, select fresh tumor tissue without necrosis, wash five times with normal saline, gently grind to a cell suspension with a tissue homogenizer, filter with a 150-mesh cell sieve, count viable tumor cells, prepare tumor cell suspension, and The concentration is 1×10 7 / ml, subcutaneously inoculate 0.1ml (1×10 6 / ml).

[0024] (2) Experimental results ...

example 3

[0026] Example 3 polymyocyte complex to the acute toxic response of mice to mice

[0027] The heart, liver, spleen, lung, kidney, pancreas, stomach, small intestine, colon, thyroid, brain, and cerebellum of animals in the Polymyocyte compound injection group were fixed with 10% neutral formalin, Conventional dehydrated paraffin-embedded sections, HE staining, and ordinary optical microscope observation revealed no pathological changes related to drugs.

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Abstract

The invention relates to a preparation method and new application of a polyinosinic preparation. Kanamycin and calcium ions are added on the basis of polyinosinic, so that the stability of polyinosinic double-stranded ribonucleic acid (RNA) is enhanced. Animal experiment results show that a polyinosinic complex has the effect of inhibiting tumor growth.

Description

Technical field: [0001] The invention relates to a preparation method of a polymyocyte complex and its new application, in particular to the antitumor effect of the polymyocyte complex containing kanamycin and calcium ions. Background technique: [0002] Polyinocyte (PIC) is a double-chain product of artificially synthesized PolyI and PolyC paired. Bacterial and protozoan infection and other functions. Domestic polykinocytes have no obvious side effects and have been used clinically to treat viral infectious diseases. [0003] A large number of literatures have reported that polymyocytes can inhibit tumors by inducing interferon and coordinating the function of immune cells, and have obvious effects on mouse sarcoma S180, Lewis lung cancer, mouse liver cancer and prostate cancer. inhibition. It is generally believed that the anti-tumor mechanism of polymyocytes mainly includes the following points: [0004] (1) Protein kinase activation: The activation of protein kinases...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K33/06A61K31/787A61P35/00A61K31/7036
Inventor 焦月侯本晶王国新王智佳沈丽丽
Owner TIANJIN JIMINGSHENG BIOLOGICAL TECH
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