Preservation solution for animal tissues and cells at low temperature, and using method thereof

A technology of animal tissue and solution, which is applied in the field of preservation of animal tissue and cells, can solve problems such as solutions that have not yet been seen, and achieve the effects of convenient use, maintenance of viability and biological activity, and improvement of success rate

Active Publication Date: 2018-11-30
WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] At present, there are no reports of in vitro preservation solutions for animal tissues and cells that meet all the above requirements

Method used

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  • Preservation solution for animal tissues and cells at low temperature, and using method thereof
  • Preservation solution for animal tissues and cells at low temperature, and using method thereof
  • Preservation solution for animal tissues and cells at low temperature, and using method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] The preparation of the in vitro preservation solution of embodiment 1 animal tissue and cell

[0021] Prepare animal tissue and cell preservation solutions according to the component ratios in Table 1. Each experimental group is configured according to the components and ratios within the protection scope of the claims of the application, and each control group is a certain component missing or group The content in weight percentage is beyond the protection scope of the claims of the present application.

[0022] Table 1 The composition of the preservation solution of animal tissues and cells and the parameters of ultrasonic irradiation treatment

[0023]

[0024]

[0025] Note: " / " means that the item does not exist, and "*" means that the index is beyond the protection scope of the claims of this application.

Embodiment 2

[0026] Example 2 Activity evaluation after treatment with mouse hepatocyte preservation solution

[0027] In this example, the animal tissue and cell preservation solution prepared in Example 1 was used to evaluate the preservation effect of primary mouse hepatocytes.

[0028] Using the two-step collagenase perfusion method, the primary mouse hepatocytes were isolated and equally divided into several parts, which were respectively placed in the preservation solutions of different experimental groups and control groups in Table 1. Cultivate in different incubators at ℃ and 37℃ for 6 days, replace the storage solution every 24 hours, and immediately perform ultrasonic irradiation treatment after changing the solution (see Table 1 for operating parameters), and use trypan blue staining method to evaluate mouse liver cells after 6 days activity, the results are shown in Table 2.

[0029] From the results in Table 2, it can be seen that the activity of mouse hepatocytes in each ex...

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Abstract

The invention relates to a preservation solution for animal tissues and cells at a low temperature, and a using method thereof. One or more of water, polyol, a Collin's solution, a UW solution, an HTKsolution and Euro-collin's solution is used as a base solution, polyphenol, citric acid, poloxamer and vitamin E are added, and irradiation treatment using ultrasonic waves having a wavelength of 20kHz to 1 MHz and a sound intensity of 3 W/cm<2> or below adjusting the preservation solution is combined with the adjustment of ratio of different components in the preservation solution and control of the wavelength, the sound intensity and the irradiation time of the ultrasonic waves in order to improve the survival ability and the biological activity of animal tissues and cells in a low temperature environment.

Description

technical field [0001] The invention relates to a method for preserving animal tissue and cells, in particular to an in vitro preservation solution for animal tissue cells in a low-temperature environment and a method for using the same. Background technique [0002] Usually, people use low temperature or cryopreservation to maintain the activity of animal tissues and cells, but it has been reported that the in vitro preservation solutions of animal tissues and cells tend to reduce the original tissue and cell activity. Studies have found that the activity of animal tissues or cells preserved by cryopreservation or cryopreservation is significantly reduced, which cannot meet the needs of clinical treatment. [0003] In recent years, a variety of in vitro preservation solutions for animal tissues and cells have been developed, but these in vitro preservation solutions are not ideal for the preservation of animal tissues and cells, not only for a short storage time, but also f...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0215A01N1/0221A01N1/0226A01N1/0294
Inventor 赵应征鲁翠涛傅红兴徐荷林姚情诸葛德力沈碧欣朱群燕杨外庚
Owner WENZHOU MEDICAL UNIV
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