Method for evaluating sterilization effect of chlorine dioxide gas under states of low concentration and long time

A technology of chlorine dioxide and bactericidal effect, which is applied in the field of evaluation of bactericidal effect of chlorine dioxide gas at low concentration for a long time, can solve problems such as evaluation methods without chlorine dioxide gas bactericidal effect, and achieve prolongation of storage time, The effect of ensuring accuracy

Active Publication Date: 2019-12-31
王妍彦 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for evaluating the bactericidal effect of chlorine dioxide gas under low concentration and long-term state, aiming to ...

Method used

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  • Method for evaluating sterilization effect of chlorine dioxide gas under states of low concentration and long time
  • Method for evaluating sterilization effect of chlorine dioxide gas under states of low concentration and long time
  • Method for evaluating sterilization effect of chlorine dioxide gas under states of low concentration and long time

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0111] The verification of the effect of the improved carrier on the survival of the test bacteria, the test steps are as follows:

[0112] (1) prepare bacterium suspension, described test bacterium is Staphylococcus aureus (ATCC 6538) and Candida albicans (ATCC10231), get Staphylococcus aureus and Candida albicans 3rd~8 generation slant fresh cultures, use TPS The bacteria content is about 5×10 2 CFU / mL~3×10 3 CFU / mL bacterial suspension;

[0113] (2) prepare the plate carrier, the culture medium of the plate carrier, take the total mass of the common nutrient agar medium as 100%, which contains 1% glycerol in a mass percentage, wherein, select directly 9 cm plate, the amount of medium added is 60-70mL;

[0114] (3) Provide a disinfection aerosol chamber, the concentration of chlorine dioxide in the aerosol chamber is 0.28mg / m 3 , smear the prepared bacterial suspension on the plate carrier, put it into the aerosol chamber, and turn on the fan circulation; after acting fo...

Embodiment 2

[0133] Neutralizer identification test, the test steps are as follows:

[0134] (1) prepare bacterium suspension, described test bacterium is Staphylococcus aureus (ATCC 6538) and Candida albicans (ATCC10231), get Staphylococcus aureus and Candida albicans 3rd~8 generation slant fresh cultures, use TPS The bacteria content is about 5×10 2 CFU / mL~3×10 3 CFU / mL bacterial suspension;

[0135] (2) Three groups of improved test carriers are provided, the improved test carriers are plate culture medium with a thickness of 1.5 cm, wherein, the two groups of culture media include glycerol and neutralizing reagents; one group of culture media does not contain neutralizing agents. And the reagent is the blank control group;

[0136] (3) Carry out three groups of experiments, including neutralizing agent group, neutralizing product group and blank control group, specifically as follows:

[0137] Neutralizer group: Use a pipette to draw a suitable dilution of the bacterial suspension ...

Embodiment 3

[0151] Plate aerosol sterilization test

[0152] (1) At a specific time, after the bacterial suspension of the same concentration is treated, the plate aerosol sterilization test is carried out. The test steps are as follows:

[0153] (1) Prepare bacterial suspension, the test bacteria are Staphylococcus aureus (ATCC 6538), Escherichia coli and Candida albicans (ATCC 10231), get the 3rd to 8th generation of Staphylococcus aureus, Escherichia coli and Candida albicans Slant fresh culture, made with TPS, with a bacterial content of about 5×10 2 CFU / mL~3×10 3 CFU / mL bacterial suspension;

[0154] (2) Two groups of improved test carriers are provided, the improved test carriers are plate culture medium, and the thickness of the culture medium is 1.6 centimeters, wherein, said one group of culture medium comprises glycerol and neutralizing reagent is test group; One group of culture medium No neutralizing reagent was used as the control group;

[0155] (3) Appropriate concentra...

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PUM

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Abstract

The invention provides a method for evaluating the sterilization effect of a chlorine dioxide gas under states of low concentration and long time. The method comprises the following steps: providing an improved test carrier, determining the time for disinfection treatment, adding a neutralizing reagent with corresponding addition mass into the improved test carrier, and applying a bacterial suspension of a test bacterium to the improved test carrier through coating to obtain a first improved test carrier; and providing a chlorine dioxide gas environment having a concentration of 0.01-0.28mg/m<3>, disinfecting the first improved test carrier in the chlorine dioxide gas environment to obtain a second improved test carrier; and culturing the second improved test carrier in an incubator, recording the number of growing bacterial colonies and calculating a sterilization rate.

Description

technical field [0001] The invention relates to the field of biology, in particular to a method for evaluating the bactericidal effect of chlorine dioxide gas at a low concentration for a long time. Background technique [0002] At present, domestic laboratory evaluation of the disinfection effect on bacteria-contaminated carriers generally refers to the most classic carrier quantitative killing test method in the "Disinfection Technical Specifications" (2002 Edition), that is, to evaluate the bactericidal effect of disinfectants. Bacteria carrier tablets are soaked in a certain amount of disinfectant for bactericidal effect, after a predetermined time, take out the bacteria tablets and add neutralizer, count the number of colonies at the same time as the control group, and calculate the kill logarithm value; evaluate the disinfection effect of the gas, the same is first Prepare the bacteria-contaminated carrier bacteria slices, put the bacteria slices into the gas disinfect...

Claims

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Application Information

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IPC IPC(8): C12Q1/18C12N1/20C12N1/16C12R1/445C12R1/725C12R1/19
CPCC12Q1/18C12N1/20C12N1/16G01N2333/31G01N2333/40Y02A50/20
Inventor 王妍彦吴予奇吴雨芯
Owner 王妍彦
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