Separating and screening method of source sludge reduction strain

A technology for separation and screening of strains is applied in the field of residual sludge treatment in sewage treatment plants, which can solve the problems of immature strain screening methods of sludge, and achieve the effect of avoiding secondary pollution and reducing output.

Inactive Publication Date: 2020-07-31
BEIJING ZHINENG XIANGYING ENERGY SAVING & ENVIRONMENTAL PROTECTION TECH CO LTD
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Problems solved by technology

However, the screening method for strains that can consume sludge is not mature, and it is a hot issue in current research.

Method used

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  • Separating and screening method of source sludge reduction strain
  • Separating and screening method of source sludge reduction strain

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Embodiment 1

[0029] A method for isolating and screening bacterial strains for reducing sludge at source, including the isolation of bacterial strains and further screening of bacterial strains.

[0030] Isolation of strains such as figure 1 Shown: Take the fresh sludge from the sewage treatment plant and put it into a 500mL Erlenmeyer flask, seal the mouth of the Erlenmeyer flask with aluminum foil to prevent evaporation, then shake it in a shaker at 30°C and 60 rpm for one week. Then remove 1 / 2 of the original sludge, add the same volume of fresh sludge, and carry out the same steps 8 times. Select the domesticated activated sludge, and carry out gradient dilution for 10 -6 、10 -7 、10 -8 、10 -9 、10 -10 、10 -11 、10 -12 , spread on sterilized agar plate medium containing sludge (preparation method: 50 g centrifugal sludge, 300 mL sterile water, 4.5 g agar, mix well, put in 500 mL Erlenmeyer flask and place in autoclaved Sterilization in the bacteria pot, after sterilization, invert...

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Abstract

The invention discloses a separating and screening method of a source sludge reduction strain. The separating and screening method of the source sludge reduction strain comprises separation of strainsand further screening of the strains, and comprises the following steps: taking fresh sludge of a sewage treatment plant, loading the fresh sludge of the sewage treatment plant into a glass container, conducting sealing, and conducting shaking culture to obtain acclimated activated sludge; coating a sludge-containing sterile agar plate culture medium with supernate, subjected to gradient dilution, of the activated sludge, and after inverted culture is conducted, selecting strains, with transparent circles, around colonies for plate streaking purification to obtain purified bacteria; and taking the separated purified bacteria, activating the separated purified bacteria on LB culture media respectively, taking the strains at the same growth stage, adding the strains at the same growth stageinto glass containers containing sludge with a certain concentration with adding amounts controlled to be the same, carrying out a shake test at a normal temperature, conducting sampling at a fixed interval, comparing obtained samples and a sample without strains, recording data, and conducting plotting according to the data to obtain a dominant strain. According to the dominant strain screened out by means of the separating and screening method of the source sludge reduction strain, without any optimization treatment, the largest sludge removal rate is higher than that of the blank sample by12.2%.

Description

technical field [0001] The invention belongs to the technical field of sludge treatment, and in particular relates to the treatment of excess sludge in a sewage treatment plant. By screening dominant bacterial strains and using endogenous consumption, the sludge can be reduced at the source. Background technique [0002] In the 21st century with the rapid development of industry, the expansion of the city and the increase of the population have continuously increased the consumption of domestic water and industrial water, which has aggravated the difficulty of sewage treatment. In the water treatment process, activated sludge process (Activated sludge process) ) is the mainstream technology for municipal sewage and industrial wastewater treatment. More than 90% of municipal sewage and more than 50% of industrial wastewater are treated by activated sludge method. The formation of activated sludge microbial micelles is the key to the success of this "century-old process". Micr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/02C12N1/20C12N1/14C12R1/01C12R1/07C12R1/645
CPCC12N1/02C12N1/20C12N1/14
Inventor 李业云王智王国臣
Owner BEIJING ZHINENG XIANGYING ENERGY SAVING & ENVIRONMENTAL PROTECTION TECH CO LTD
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