Congo red staining method

Abstract

The invention discloses a Congo red staining method comprising steps as follows: a sodium carboxymethyl cellulose agar plate medium is coated and inoculated with bacteria, the plate medium is placed in a constant-temperature incubator for culture, and single bacterial colonies with clear morphology grow; target bacterial colonies are marked out according to the morphology of the bacterial colonies; the plate medium where the colonies grow is soaked with a Congo red solution with the concentration of 2-5 mg / mL for 0.5-1 min, and then the Congo red solution is removed; the plate medium is washed with an added sodium chloride solution with the concentration of 1 mol / L for 1-3 times and then soaked with the sodium chloride solution with the concentration of 1 mol / L for 3-5 min, then the sodium chloride solution is poured out, and a yellowish transparent zone can appear around the colonies producing cellulase. Staining and soaking time is shortened, so that the dispersion of the colonies is reduced, the possibility of cross contamination is decreased, and time is saved.

Description

technical field [0001] The invention relates to a microbial dyeing method, in particular to a dyeing method for screening cellulase-producing bacteria in microbial experiments. Background technique [0002] The staining method for screening cellulase-producing bacteria in existing microbial experiments is to cover the culture medium with a concentration of 1 mg / mL Congo red solution, soak for 10-15 minutes or longer, and then pour off the Congo red Solution, then add a sodium chloride solution with a concentration of 1 mol / L to soak for 15 minutes or longer, and then pour off the sodium chloride solution. At this time, a transparent circle will appear around the colony capable of producing cellulase. The Congo red staining method specifically refers to the cellulose staining method, and all microorganisms capable of producing cellulase can be identified by this method. In the prior art, if the concentration of Congo red is low and the dyeing time is short, the color cannot ...

AI Technical Summary

Problems solved by technology

In the prior art, if the concentration of Congo red is low and the dyeing time is short, the color cannot be dyed, and if the soaking time is short, the transparent circle is not obvious. Therefore, the existing method takes a long time to dye because of the low concentration of Congo red used. Color, at the same time soaking and elution time is also too long, staining and soaking time is too long, it will cause the colony to disperse, mix between the colonies, easy to cross-contamination, not only prolong the experiment time, but also may cause unnecessary trouble to the experiment, if During the staining process, the colonies disperse very badly, and if there is crossover between different colonies, the colonies must be transferred out before staining, and then stained and screened. The steps are cumbersome

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