The invention relates to anti-counterfeiting stamp-pad ink and a preparation method thereof. The invention aims at providing environmentally-friendly anti-counterfeiting stamp-pad ink suitable for long-term use for anti-counterfeiting, which has good weather fastness and corrosion resistance and a preparation method thereof. The anti-counterfeiting stamp-pad ink comprises solvents, resin and an anti-counterfeiting material. The preparation method comprises the following steps: uniformly mixing and stirring the solvents to obtain a mixed solvent; adding 1 / 2 mixed solvent into a reaction kettle, heating, maintaining a constant temperature, adding the resin, continuing to stir after the resin is completely dissolved, cooling the mixture to the room temperature to obtain a resin solution; uniformly mixing a part of resin solution with the mixed solvent, adding the anti-counterfeiting material, pigment or dope in the mixed solution, and injecting the mixture into a grinding machine for grinding to obtain color sizing; and adding the rest resin solution, the rest mixed solution and other additives into the color sizing, stirring and standing to obtain a product. The product prepared by the invention can be printed on various materials, the formed film is uniform, unlikely to drop, excellent in acid and alkali resistance and weather fastness and short in drying time, thereby being suitable for long-term anti-counterfeiting, particularly suitable for anti-counterfeiting of precious painting.