A dual-channel chromogenic direct-reading thin-layer chromatography plate and its preparation method and application

A thin-layer chromatography, dual-channel technology, applied in the direction of analytical materials, instruments, measuring devices, etc., can solve problems such as limited applications, and achieve the effects of increased sensitivity, high-sensitivity color development, and convenient use

Active Publication Date: 2018-12-18
BIOHIT BIOTECH HEFEI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These characteristics limit its application in the field environment and low-cost rapid detection and screening

Method used

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  • A dual-channel chromogenic direct-reading thin-layer chromatography plate and its preparation method and application
  • A dual-channel chromogenic direct-reading thin-layer chromatography plate and its preparation method and application
  • A dual-channel chromogenic direct-reading thin-layer chromatography plate and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0045] Example 1 Preparation of Alizarin Red@Nano Zirconium Dioxide Compound

[0046] Such as figure 1 As shown, the preparation of alizarin red@nano zirconia complex:

[0047] (1) Prepare 100 mM tetrabutyl zirconate solution, the solvent is toluene / ethanol solution (v:v=1:1), and stir at room temperature for one hour.

[0048](2) Put flexible fiber paper, which can also be one of face tissue and quantitative filter paper, in a suction filtration device, rinse it with ethanol for 2-3 times, remove the moisture therein, and dry it with air flow. Pipette about 10 mL of the tetrabutyl zirconate solution into the suction filtration device, and let it stand for 3 minutes, so that the tetrabutyl zirconate solution is fully soaked in the cellulose paper.

[0049] (3) Turn on the suction filtration device to slowly filter the tetrabutyl zirconate solution through the cellulose paper, then add ethanol for 2-3 rinses, and let stand for 2 minutes.

[0050] (4) Use a suction filter to ...

Embodiment 2

[0053] Example 2 Preparation of phosphomolybdic acid@nano-titanium dioxide composite

[0054] Such as figure 1 As shown, the preparation of phosphomolybdic acid@nano-titanium dioxide composite:

[0055] (1) Prepare a 100 mM tetrabutyl titanate solution, the solvent is a toluene / ethanol solution (v:v=1:1), and stir at room temperature for one hour.

[0056] (2) Put flexible fiber paper, which can also be one of face tissue and quantitative filter paper, in a suction filtration device, rinse it with ethanol for 2-3 times, remove the moisture therein, and dry it with air flow. Pipette about 10 mL of tetrabutyl titanate solution into the suction filtration device, and let stand for 3 minutes, so that the tetrabutyl titanate solution is fully soaked in the cellulose paper.

[0057] (3) Turn on the suction filtration device to slowly filter the tetrabutyl titanate solution through the cellulose paper, then add ethanol for 2-3 rinses, and let stand for 2 minutes.

[0058] (4) Use ...

Embodiment 3

[0061] Embodiment 3 prepares the thin-layer plate containing alizarin red (phosphomolybdic acid) / nano zirconium dioxide (titanium) composite as developer

[0062] Take a thin-layer chromatography strip of aluminum base, paper base or plastic base material, the size is about 10cm*5cm. Firstly, the position of the ratio shift value of the substrate to be tested is determined, which is accomplished by thin-layer chromatography-iodine chromogenic method. After determining the position, print 5 microliters of the aforementioned nano-color developer dispersions with a thin-layer strip printer at the substrate ratio shift value Rf by about 1 mm, that is, 2 mm in width, such as image 3 shown. During the printing process, the color area on the other side is covered with aluminum foil to protect it from interference. After the double color areas are printed, the middle hydrophobic partition is performed with 5% dichloromethoxysiloxane. Activate the thin-layer chromatography plate in...

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Abstract

The invention relates to the field of rapid detection and analysis for food safety, in particular to a double-channel chromogenic direct-reading thin layer chromatography plate and a preparation method and application of the double-channel chromogenic direct-reading thin layer chromatography plate. The double-channel chromogenic direct-reading thin layer chromatography plate comprises a base material and a fixed phase, a chromogenic area is arranged in a specific Rf (Retardation Factor) value position to be detected on the surface of the fixed phase, and comprises two chromogenic subareas, a hydrophobic dividing line is arranged between the chromogenic subareas, the chromogenic subareas comprise chromogenic agents@nano-metallic oxide compounds, and the chromogenic agents of the two chromogenic subareas are two types of the chromogenic agents which are different. After the chromogenic area is determined on the thin layer chromatography base material, the two chromogenic agents are respectively printed in the two chromogenic subareas, and divided by a hydrophobic solvent, and then dried, activated and manufactured into a product. The product disclosed by the invention is high in sensitivity, simple in manufacturing method, convenient and fast to use and can realize the qualitative and semi-quantitative treatment of a detected substrate, thereby being widely used in the detection of drug residues.

Description

technical field [0001] The invention relates to the field of rapid detection and analysis of food safety, in particular to a dual-channel color development direct-reading thin-layer chromatography plate and its preparation method and application. Background technique [0002] As a classic separation and analysis method, TLC analysis is widely used in the fields of natural products, drugs, food analysis and so on. In recent years, some researchers have developed some rapid food safety detection technologies based on high-performance thin-layer chromatography separation methods, and successfully detected a series of harmful residues in food such as mycotoxins, sulfa drugs, and illegally added drugs in health food. However, since the harmful residues in food usually appear in low concentrations, the lack of high-sensitivity and reproducible direct-reading color development methods has become the main obstacle hindering the application of high-performance thin-layer chromatograp...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/90
CPCG01N30/90
Inventor 张宜明刘峰
Owner BIOHIT BIOTECH HEFEI
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