Liquid drop micro-fluidic chip used for detecting diabetes with high sensitivity and detection method

Abstract

The invention relates to a liquid drop micro-fluidic chip used for detecting the diabetes with high sensitivity and a detection method, and belongs to the technical field of microfluidics. The liquiddrop micro-fluidic chip sequentially comprises a cover glass layer 20, an upper fluid passageway layer 30, a lower fluid passageway layer 40 and a base layer 50; and a liquid drop micro-fluidic chip based high sensitivity diabetes detection device comprises a high pressure liquid phase chromatographic pump 2, a sample inlet valve 3, the liquid drop micro-fluidic chip 6, a deuterium lamp and a halogen tungsten lamp source 10, an ultraviolet-visible light spectrometer 11 and data processing software 13. The provided micro-fluidic chip is small in size, low in cost and simple in operation, and isfavorable for real-time detection on the site. The high sensitivity diabetes detection method is not only suitable for the high sensitivity detection of a sample HbA1c, but also suitable for detection of biomacromolecules like polypeptide, nucleic acid and protein.

Description

technical field

[0001] The invention relates to a droplet microfluidic chip and a detection method for high-sensitivity detection of diabetes, which belong to the technical field of microfluidics and also belong to the technical field of medical equipment. Background technique

[0002] Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia with a high incidence rate. It is one of the four major diseases that endanger human health. Long-term diabetes can easily lead to complications such as diabetic ketosis, cardiovascular disease, neuropathy, diabetic foot, and diabetic nephropathy, which seriously threaten human health. According to the 2013 announcement by the Diabetes Society of the Chinese Medical Association, it is estimated that there are 139 million diabetics in my country, of which 60.7% are undiagnosed.

[0003] For many years, the diagnosis of diabetes has been based on plasma glucose levels, including fasting blood glucose and oral gluc...

Examples

Embodiment 1

[0032] refer to figure 2 , a droplet microfluidic chip for high-sensitivity detection of diabetes in the present embodiment, the material is PMMA, which includes four layers of simple planar structure: cover sheet layer 20, upper fluid channel layer 30, lower fluid channel layer 40, base layer 50. The thickness of the cover sheet layer 20 and the base layer 50 are both 2 mm, the thickness of the upper fluid channel layer 30 and the lower fluid channel layer 40 are both 1 mm, the overall polymer column is about 50 mm long, 0.5 mm wide, and 0.5 mm high; the droplet generator cross Channel height 0.5mm, W 1 =W 2 =W 3 =W 4 =0.2mm, W=0.5mm; the diameter of the micro detection cell is 1mm, and the height is 2mm; the diameters of the water phase inlet 21, the first oil phase inlet 22, the second oil phase inlet 23 and the fluid outlet 24 are all 1.6mm.

[0033] In this example, the preparation process of a strong cation-exchange polymer monolithic column in a droplet microfluidi...

Embodiment 2

[0037] refer to figure 2 , a droplet microfluidic chip for high-sensitivity detection of diabetes in this embodiment, the material is COC, the thickness of the cover layer 20 and the base layer 50 are both 2 mm, the upper fluid channel layer 30 and the lower fluid channel layer The thickness of 40 is 0.5mm, the overall polymer column is about 30mm long, 0.5mm wide, and 0.3mm high; the cross channel of the droplet generator is 0.3mm high, W 1 =W 2 =W 3 =W 4 =0.3mm, W=0.5mm; the diameter of the micro detection cell is 0.6mm, and the height is 1mm; the diameters of the water phase inlet 21, the first oil phase inlet 22, the second oil phase inlet 23 and the fluid outlet 24 are all 1.0mm.

[0038]The preparation process of a strong cation-exchange polymer monolithic column in a droplet microfluidic chip for high-sensitivity detection of diabetes in this example: the strong cation-exchange polymer monolithic column was prepared by thermally induced polymerization. The first ste...

technical field

[0001] The invention relates to a droplet microfluidic chip and a detection method for high-sensitivity detection of diabetes, which belong to the technical field of microfluidics and also belong to the technical field of medical equipment. Background technique

[0002] Diabetes mellitus is a group of metabolic diseases characterized by hyperglycemia with a high incidence rate. It is one of the four major diseases that endanger human health. Long-term diabetes can easily lead to complications such as diabetic ketosis, cardiovascular disease, neuropathy, diabetic foot, and diabetic nephropathy, which seriously threaten human health. According to the 2013 announcement by the Diabetes Society of the Chinese Medical Association, it is estimated that there are 139 million diabetics in my country, of which 60.7% are undiagnosed.

[0003] For many years, the diagnosis of diabetes has been based on plasma glucose levels, including fasting blood glucose and oral gluc...

Examples

Embodiment 1

[0032] refer to figure 2 , a droplet microfluidic chip for high-sensitivity detection of diabetes in the present embodiment, the material is PMMA, which includes four layers of simple planar structure: cover sheet layer 20, upper fluid channel layer 30, lower fluid channel layer 40, base layer 50. The thickness of the cover sheet layer 20 and the base layer 50 are both 2 mm, the thickness of the upper fluid channel layer 30 and the lower fluid channel layer 40 are both 1 mm, the overall polymer column is about 50 mm long, 0.5 mm wide, and 0.5 mm high; the droplet generator cross Channel height 0.5mm, W 1 =W 2 =W 3 =W 4 =0.2mm, W=0.5mm; the diameter of the micro detection cell is 1mm, and the height is 2mm; the diameters of the water phase inlet 21, the first oil phase inlet 22, the second oil phase inlet 23 and the fluid outlet 24 are all 1.6mm.

[0033] In this example, the preparation process of a strong cation-exchange polymer monolithic column in a droplet microfluidi...

Embodiment 2

[0037] refer to figure 2 , a droplet microfluidic chip for high-sensitivity detection of diabetes in this embodiment, the material is COC, the thickness of the cover layer 20 and the base layer 50 are both 2 mm, the upper fluid channel layer 30 and the lower fluid channel layer The thickness of 40 is 0.5mm, the overall polymer column is about 30mm long, 0.5mm wide, and 0.3mm high; the cross channel of the droplet generator is 0.3mm high, W 1 =W 2 =W 3 =W 4 =0.3mm, W=0.5mm; the diameter of the micro detection cell is 0.6mm, and the height is 1mm; the diameters of the water phase inlet 21, the first oil phase inlet 22, the second oil phase inlet 23 and the fluid outlet 24 are all 1.0mm.

[0038]The preparation process of a strong cation-exchange polymer monolithic column in a droplet microfluidic chip for high-sensitivity detection of diabetes in this example: the strong cation-exchange polymer monolithic column was prepared by thermally induced polymerization. The first ste...

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