The invention discloses a
tetrazole determination method for viability of sweet pepper seeds, and belongs to the technical field of quality inspection of
crop seeds. The
tetrazole determination method for viability of the sweet pepper seeds comprises the following steps that clean sweet pepper seeds are soaked in clear water for 12-18 hours, and then taken out; one third of the seeds are longitudinally
cut in the direction of radicles and
cotyledon tips along a flat plane, meanwhile, seed coats close to base parts of the seeds are
cut off, the radicles are not damaged, and therefore
embryo cavities of the seeds are opened; the treated seeds are put into a
tetrazole solution which is prepared from a
phosphate buffer solution and has a
mass concentration of 1.0%, and dyed for 6-12 hours in a dark place at a constant temperature of 30-35 DEG C; and after
dyeing is finished, the seeds are washed with
tap water for 2-3 times, and the flat surfaces of the seeds are extruded with
tweezers, so that the radicles are extruded from the
embryo cavities along notches of the base parts, and whether the seeds have viability or not is judged according to the coloring condition of the radicles. The invention provides a simple and feasible method for inspecting the quality of the sweet pepper seeds, the identification standard is visual, the result is reliable, and the seed quality identification in the production and circulation of the sweet pepper seeds is convenient.