Tetrazole determination method for viability of sweet pepper seeds

Abstract

The invention discloses a tetrazole determination method for viability of sweet pepper seeds, and belongs to the technical field of quality inspection of crop seeds. The tetrazole determination method for viability of the sweet pepper seeds comprises the following steps that clean sweet pepper seeds are soaked in clear water for 12-18 hours, and then taken out; one third of the seeds are longitudinally cut in the direction of radicles and cotyledon tips along a flat plane, meanwhile, seed coats close to base parts of the seeds are cut off, the radicles are not damaged, and therefore embryo cavities of the seeds are opened; the treated seeds are put into a tetrazole solution which is prepared from a phosphate buffer solution and has a mass concentration of 1.0%, and dyed for 6-12 hours in a dark place at a constant temperature of 30-35 DEG C; and after dyeing is finished, the seeds are washed with tap water for 2-3 times, and the flat surfaces of the seeds are extruded with tweezers, so that the radicles are extruded from the embryo cavities along notches of the base parts, and whether the seeds have viability or not is judged according to the coloring condition of the radicles. The invention provides a simple and feasible method for inspecting the quality of the sweet pepper seeds, the identification standard is visual, the result is reliable, and the seed quality identification in the production and circulation of the sweet pepper seeds is convenient.

Description

technical field [0001] The invention relates to the field of crop seed quality inspection, in particular to a method for measuring the viability of sweet pepper seeds. Background technique [0002] Seed viability refers to the potential ability of the seed to germinate or the vitality of the embryo, which is expected to have the potential to grow into a normal seedling. Many plant seeds have dormant characteristics, and even if suitable germination conditions are given after harvest, they cannot germinate or have a low germination rate. But in fact, most of the seeds that do not germinate may have vitality, and the dormant seeds still have normal physiological and biochemical metabolic characteristics. Therefore, the seed viability assay method can be used to show the life phenomenon and vitality of dormant seeds, and to judge its potential ability to grow normal seedlings and plants to indicate its physiological quality. The determination of seed viability can not only un...

AI Technical Summary

Problems solved by technology

In my country, sweet peppers are planted in a wide area and the amount of seeds used is large, but sweet pepper seeds are less resistant to storage and are prone to natural aging; and because some seeds may have dormancy, the existing germination test technology cannot guarantee all vitality. All the seeds germinated, it is impossible to accurately evaluate the vigor of sweet pepper seeds and predict the maximum germination potential

Images