DNA silver staining method
A technology of dyeing agent and distilled water, which is applied in the field of DNA silver staining, can solve the problems of reducing the contrast between DNA band pattern and background, unsuitable DNA recovery, and complicated operation steps, so as to reduce the types of solution preparation, promote the development ability, and the color development process fast effect
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Embodiment 1
[0023] The steps of DNA silver staining method of the present invention are as follows:
[0024] (1) Cut off the power supply after electrophoresis, pour out the buffer solution from the electrophoresis tank, then remove the rubber plate, put it into a porcelain plate filled with distilled water, and rinse it twice with distilled water;
[0025] (2) Silver staining: adding a staining agent for silver staining, and shaking it by hand or on a shaker, staining for 10 minutes, and then rinsing twice with distilled water, wherein the staining agent is made of 0.2% AgNO 3 (W / V) and 10% absolute ethanol (V / V);
[0026] (3) Development: add a developer for color development, and shake it by hand or on a shaker, develop for 10 minutes until the target band is clear, wherein the developer consists of 3% NaOH (W / V) and 0.3% formaldehyde (V / V) composition;
[0027] (4) Rinse twice with distilled water;
[0028] (5) Gel photography: use a digital camera to take pictures of the gel laid ...
Embodiment 2
[0056] The steps of DNA silver staining method of the present invention are as follows:
[0057] (1) Cut off the power supply after electrophoresis, pour out the buffer solution from the electrophoresis tank, then remove the rubber plate, put it into a porcelain plate filled with distilled water, and rinse it twice with distilled water;
[0058] (2) Silver staining: adding a staining agent for silver staining, and shaking it by hand or on a shaker, staining for 9 minutes, and then rinsing twice with distilled water, wherein the staining agent is composed of 0.2% AgNO 3 (W / V) and 10% absolute ethanol (V / V);
[0059] (3) Developing: Add a developer for color development, and shake it by hand or on a shaker, develop for 8 minutes until the target band is clear, wherein the developer consists of 2% NaOH (W / V) and 0.4% NaOH Formaldehyde (V / V) composition;
[0060] (4) Rinse 3 times with distilled water;
[0061] (5) Gel photography: Use a digital camera to take pictures of the g...
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