143results about How to "Fast color development" patented technology

The invention relates to a semitransparent fluorescent powder/glass composite luminescent ceramic wafer and a preparation method thereof. The semitransparent fluorescent powder/glass composite luminescent ceramic wafer is obtained by carrying out 'pelletizing, compression moulding and sintering' on fluorescent powder and low-melting-point glass powder, wherein content of the fluorescent powder is 30-85wt%, and the content of the low-melting-point glass powder is 70-15wt%. The preparation method of the semitransparent fluorescent powder/glass composite luminescent ceramic wafer comprises the following steps: firstly, uniformly mixing fluorescent powder with glass powder in certain proportion, and adding a binding agent required by pelletizing and demoulding, so that pelletized powder of 60-100 meshes is obtained; secondly, carrying out compression moulding on the obtained pelletized powder by virtue of a mould, so that a green body of a certain shape is obtained; thirdly, carrying out heat treatment, namely carrying out glue drainage on the green body for 2-4 hours at the temperature of 300-395 DEG C, and sintering for 1-2 hours at the temperature of 395-410 DEG C, wherein the whole heat treatment process is carried out in the air atmosphere; and finally the fluorescent powder/glass composite luminescent ceramic wafer is obtained.

A sensitivity raising detection method of colloid gold immunity chromatography method comprises: when using general colloid gold immunity chromatography to check object sample, adding an object sample, adding signal amplifying agents of 10 to 80ul between the gold label antibody layer and the detection line; processing the detection line obtained by general colloid gold immunity chromatography method; reacting under room temperature for 1 to 10min; if the object sample contains antigen, the detection carrier is formed with a detection line and a control line as clear black or grey black color, to represent positive property, or if the object sample not contains antigens, the control line is black or grey black color, and the detection line has not color, to represent negative property. The method can significantly improve the sensitivity of colloid gold immunity chromatography reagent kit on the detection of biological macromolecules, having fast color development, lower background interference compared with general silver stain method, wide application prospect and high development value.

The invention relates to a staining agent combination for tumor tissue cells, in particular to a staining agent combination for the tumor tissue cells. The combination comprises 0.01%-5% of a color developing agent, less than or equal to 6.5% of folic acid, 0.1%-10% of a leuco agent, less than or equal to 10% of a pH modifier and 3%-95% of water, wherein the color developing agent adopts methylene blue and/or dichlorophenolindophenol; the leuco agent adopts a reducing reagent; a targeted drug preparation adopts the folic acid; and the solution pH modifier and the water are adopted. The coloring agent for staining tumor cell can also be used for detecting the tumor cells. The invention also relates to a method of the staining agent combination used for detecting the tumor cells, a kit for detecting the tumor cells and a detection carrier.

A method for the stabilization of an aqueous choline hydroxide solution includes optionally, adding a first stabilizer of a dithionite salt or a dialkylhydroxylamine to an aqueous solution containing reactants that will produce an aqueous choline hydroxide solution; and after the aqueous choline hydroxide solution is formed, adding a second stabilizer of a dithionite salt or a dialkylhydroxylamine to the aqueous choline hydroxide solution. The stabilized choline hydroxide solution may include choline hydroxide, water, and a dithionite salt and/or a dialkylhydroxylamine as a stabilizer present in an amount of about 100 ppm to about 2000 ppm by weight of the stabilized choline hydroxide solution.

The invention belongs to the technical field of biological detection, and discloses a test strip and a test method for detecting a phospholipase A2 receptor (PLA2R) antibody. The test strip for detecting the PLA2R antibody comprises a sample pad, a conjugate pad 1, a conjugate pad 2, an NC film and absorbent paper which are sequentially in lap joint with a viscous bottom plate, wherein the conjugate pad 1 is sprayed with a PLA2R biotinylated conjugate; the conjugate pad 2 is sprayed with an SA fluorescent microsphere conjugate and a DNP-BSA fluorescent microsphere conjugate; the NC film is enveloped with a capture body as a detection line and is enveloped with an anti-DNP-BSA antibody as a quality control line. The test strip for detecting the PLA2R antibody by using a capture method has afaster color development speed, and only 7 minutes is needed for sample feeding to result reading, so that the detection time is shorter; the test strip is stronger in coloring signals, the missing detection of weak positive does not easily occur, and the specificity is better; the test strip and the test method can be used for quantitatively, accurately and conveniently testing the content of the PLA2R antibody in human serum, plasma and whole blood.

The invention discloses a detection reagent, a reagent strip and a kit for the semiquantitative detection of clenobuterol hydrochloride through a colloidal gold fusion latex method, and preparation methods thereof. Colloidal gold preparation, colloidal gold marker preparation and purification, latex particle marking, colloidal gold and latex maker immobilization, cellulose nitrate membrane coating, reagent strip preparation, and detection kit preparation are comprised in the invention. According to the invention, the cost is low, the accuracy is high, the stability is strong, the color development is rapid, uniform and stable, the use is convenient, the operation is simple, and the organic unification of a complex principle and the simple operation can be really achieved; and the kit has the characteristics of convenient preservation and long validity period.

The invention relates to a reagent and a method for rapidly detecting formaldehyde in foods. The reagent comprises a phloroglucinol solution, a sodium hydroxide solution and a formaldehyde standard solution. The detection method comprises the following steps: pretreating a sample, and preparing a sample solution to be detected; taking three colorimetric tubes, respectively adding the sample solution to be detected into one colorimetric tube, adding the formaldehyde standard solution into another colorimetric tube, and taking the rest colorimetric tube as a blank control group; adding the sodium hydroxide solution into the three colorimetric tubes, and adding the phloroglucinol solution into the three colorimetric tubes; finally, dripping the three solutions onto test paper, observing color changes of the three solutions on the test paper, comparing the color change of the sample solution to be detected with a color card, and performing qualitative and quantitative analysis on formaldehyde in the sample to be detected. The detection method is easy to operate, special equipment and instruments are not needed, the detection result is reliable, and the method has high sensitivity and is particularly suitable for rapid detection of the formaldehyde in the foods.

The invention relates to the field of new materials, and particularly discloses an application of a diarylethene compound in fingerprint fluorescence color development, the compound is used as a fluorescence developer, and the fluorescence developer has the following structural general formula: in the general formula, Ar1 is a hydrophobic aromatic ring, and Ar2 is a hydrophilic aromatic heterocyclic cation. By using the fluorescent developer solution disclosed by the invention, fingerprints on different substrates can be rapidly, safely and efficiently developed, the developed fingerprint hashigh resolution and high contrast, can clearly distinguish 1-3 levels of details of the fingerprint, can provide effective and reliable evidences for individual identification, and provides a rapid, efficient and safe fingerprint fluorescence developing technology for the fields of criminal investigation, judicial expertise, science popularization public welfare and the like.

The invention belongs to the technical field of enzyme immunity, and in particular relates to a preparation method of a polymerase-labeled antibody. The preparation method of the polymerase-labeled antibody is characterized in that it comprises the steps of: in the presence of a nonionic surfactant, soluble horseradish peroxidase (HRP) is micellized, and the sugar molecules on the surface of the HRP are first oxidized with NaIO form an aldehyde group, and then use the amino group on HRP to undergo a condensation reaction with the aldehyde group to generate a polymerase (Poly-HRP), and then use the excess aldehyde group on the polymerase to combine with the amino group on IgG to generate soluble Poly-HRP-IgG , After reducing the generated unsaturated bond with NaBH4, a stable polymerase-labeled antibody (Poly-HRP-IgG) is generated. The preparation process of the method is simple, and the prepared polymerase-labeled antibody has faster color development speed, higher sensitivity and better storage stability when applied.

The invention relates to an in-vitro simulated dissolution and continuous online detection device for nicotine in bagged oral tobacco products. The device comprises a color developing agent containing device (R1), a dissolution agent containing device (R2), a first peristaltic pump (P1), a second peristaltic pump (P2), a dissolution pool (A), a decolorizing column (B), a reaction pool (R) and a spectrophotometer (D), wherein the first peristaltic pump (P1) is positioned at the downstream of the color developing agent containing device (R1) and is connected with the color developing agent containing device (R1) by virtue of a pipeline; the second peristaltic pump (P2), the dissolution pool (A), the decolorizing column (B), the reaction pool (R) and the spectrophotometer (D) are positioned at the downstream of the dissolution agent containing device (R2) and are sequentially connected with one another; the reaction pool (R) is positioned at the downstream of the first peristaltic pump (P1) and is connected with the first peristaltic pump (P1) by virtue of a pipeline; and the spectrophotometer (D) is connected with a data processing device (C). The invention also relates to an in-vitro simulated dissolution and continuous online detection method for the nicotine in the bagged oral tobacco products. According to the detection device and method disclosed by the invention, the continuous on-line detection of in-vitro dissolution for the nicotine can be realized, the operation is convenient, and the detection is rapid.

The invention discloses a colloidal gold test strip for fast detecting IgG (immunoglobulin G) and IgM (immunoglobulin M) antibodies of dengue fever virus and belongs to medical test consumables. The colloidal gold test strip is characterized in that 1-4 kinds of antigens are enveloped on a gold labeled protein-adsorbed fiber combination pad; the antigens are selected from four subtype antigens of the dengue fever virus labeld by colloidal gold; a polyclonal antibody of the antigens for resisting the dengue fever virus is enveloped on a quality control line C; an anti-mu chain monoclonal antibody is enveloped on a detection line T1, and an anti-human IgG monoclonal antibody is enveloped on a detection line T2. The test strip disclosed by the invention can fast detect serotype 1, 2, 3 and 4 type IgG and IgM antibodies of the dengue fever virus by adopting an antibody capture method combined with an immune colloidal gold technique and has the advantages of simplicity, sensitivity, specificity and accuracy. The test strip disclosed by the invention has the advantages of small sample quantity and wide storage condition, can save resources and can judge the positive result of the IgG or IgM antibody of the dengue fever virus once at the same time.

The invention discloses Mn<4+> doped fluoride red light fluorescent powder for a high color rendering and warm white light LED (Light Emitting Diode) and a preparation method of the Mn<4+> doped fluoride red light fluorescent powder. The chemical composition of a fluorescent powder material is Rb2AF6:xMn<4+>, wherein A is Zr or Ti, x is the mol percentage coefficient of Mn<4+> doped ions relative to the A, and the x is greater than or equal to 1.25 percent and smaller than or equal to 20 percent. The fluorescent powder material has the effects of broadband absorption and narrowband emission, and can be used for packaging a high-quality LED. The preparation method comprises the following steps: at room temperature, adding K2MnF6 powder into water, and stirring until the power is completely dissolved; then adding RbF powder and H2AF6 aqueous solution, continuously stirring until the substances are dissolved, centrifuging, washing and drying to obtain an orange-yellow sediment, namely the Mn<4+> doped fluoride red light fluorescent powder for the high color rendering and warm white light LED. The preparation method disclosed by the invention has the advantages of simplicity and convenience in operation, greenness, environment friendliness, mild reaction conditions, high efficiency, quickness and suitability for industrial large-scale production.

The present invention relates to the production process of chlorine residue test paper with tetraalkyl benzidine, especially 3,3',5,5'-tetramethyl benzidine as color-developing agent. THe production process includes the steps of: compounding the soaking solution with tetraalkyl benzidine, volatile solvent and weak acid regualting agent; soaking test paper in the solution; air drying at 60 deg.c; stoving and cutting the test paper into small sheets; and adhering the sheets to base. The test can resist the pH developing effect in wide range, may be grade into 0,1,2,3,5,10 ppm grades. Being fast and stable in development, the test paper is used in the detection of disinfectant content in swimming pool.

The invention discloses a DNA silver staining method. The method comprises the following steps: (1) cutting off power supply after finishing electrophoresis, pouring a buffer solution out from an electrophoresis tank, then taking off a gel plate, putting the rubber plate into a porcelain dish filled with distilled water, and rinsing the gel plate by the distilled water for 2 to 3 times; (2) carrying out silver staining: adding a staining agent to carry out silver staining, shaking ceaselessly, staining for 8 to 12 minutes, and then rinsing by the distilled water for 2 to 3 times; (3) carrying out developing: adding a developing agent to carry out color development, shaking ceaselessly, and developing for 8 to 12 min; (4) rinsing by the distilled water for 2 to 3 times; and (5) carrying out gel shooting: spreading gelatin onto a X-ray film viewer to take a photo. The method integrates the fixing step and the staining step, adopts ethanol to fix, and overcomes the defects that the belt color is lighter when acetic acid is used for fixing, and the acetic acid has penetrating odor, causticity and so on in the prior sliver staining method. Additionally, the method does not need use ethanol and/ or acetic acid to stop developing color, omits the color development stopping step, and solves the problems of penetrating odor and causticity brought by the acetic acid and the like.

The invention discloses a high color rendering white light LED with a high S/P value, the LED comprises a blue light chip and a fluorescent powder layer used for coating the blue light chip, wherein the fluorescent powder layer comprises green fluorescent powder and read fluorescent powder. According to the high color rendering white light LED with the high S/P value provided by the invention, a color rendering value Ra is more than 80, the S/P value is more than 1.90, and other light quality parameters can also meet the performance requirement of the white light LED based on mesopic vision in existing outdoor illumination.

The tilapia fillet coloring process includes the following steps: filling water into water trough, regulating the water temperature to basically the same as that in cultivating environment, and setting tilapia inside the water trough; introducing CO and air successively through a gas-water mixing pump to mix homogeneously for 13-20 min; and cutting tilapia, sterilizing and making tilapia fillet. The said coloring process adopts live body coloring technology, and has reduced excessive CO gas on the worker, decreased sterilizing times, and raised coloring effect.

The invention discloses a waterproof carbon paper chromogenic layer coating. The coating comprises, by weight, 3-5 parts of light calcium carbonate, 2-4 parts of calcined kaolin, 1.5-1.8 parts of activated clay, 2-4 parts of corn modified starch, 0.6-0.8 parts of carboxymethylcellulose sodium, 3-5 parts of paraffin, 0.5-0.7 parts of an emulsifier, 5-8 parts of styrene-butadiene latex, 3-5 parts of polyamine polyepoxy resin, 0.5-0.8 parts of a composite dispersant, 1-2 parts of dimethicone, 3-5 parts of carboxylic styrene butadiene latex and 1.2-1.5 parts of titanium dioxide. Light calcium carbonate with fine and uniform particle size is easy to obtain, is safe and environmentally friendly, and has no pollution to human bodies or environment; the composite dispersant allows the prepared coating to have low viscosity, few bubbles and low dynamic surface tension and to reach below 40dyne; and activated clay is applied to make the coating have the advantages of fast chromogenic speed, high whiteness and uniform hue and to make a coat obtained through using the coating have smooth and flattened surface, high glossiness, and good and lasting waterproof effect.

The invention provides red fluorescent powder and a luminescent device with the red fluorescent powder. The fluorescent powder at least comprises an element M, an element A, an element D and an element R. The element M comprises one or two of Ca, Sr, Ba and Mg and can further comprise one or two of Na and K; the A element comprises Al or Al and Ga, and further comprises one or two of Sc, Y, Lu and La; the element D is selected from one or two of O, N and F and must contain O; the R element is one or two of Mn, Ce and Eu, and must contain Mn; the fluorescent powder has a crystal structure which is the same as that of Sr4Al14O25. As the ion radius of Sc < 3 + > is larger than that of Al < 3 + >, lattice distortion caused by doping of Mn < 4 + > ions can be relieved by doping the Sc < 3 + > ions, and the local environment of Mn < 4 + > is improved, so that the luminous intensity of Mn < 4 + > is enhanced. The red fluorescent powder has relatively strong absorption in ultraviolet-blue light regions, and can emit relatively strong narrow-band red light under the excitation of ultraviolet-blue light.

The invention discloses a light path heat dissipating combination system for a spotlight. The light path heat dissipating combination system comprises a light path system and a heat dissipating system, wherein the light path system comprises a COB integrated light source, a light source light receiver, an aspherical mirror and a plano-convex lens; the light source light receiver is a cylinder body of which two ends are opened; a light emergent outlet is formed in one end of the cylinder body; a conical surface concaved into the cylinder body is arranged at the light emergent outlet end; an opening is formed in the bottom of the conical surface and is used as a light incident inlet; the end face of the light incident inlet and the end face of the other end of the cylinder body are positioned on the same horizontal plane; a vacuum coating layer is arranged on the conical surface; the vacuum coating layer and the conical surface jointly form a reflection part of the light source light receiver; the light incident inlet is greater than the light emergent outlet; the aspherical mirror is fixed at the light emergent outlet of the light source light receiver by an aspherical mirror pressing plate; an aspherical mirror silicone buffer washer is arranged between the aspherical mirror pressing plate and the light source light receiver; and center lines of the plano-convex lens, the aspherical mirror, the light source light receiver and the COB integrated light source are positioned on the same axis. The light path heat dissipating combination system disclosed by the invention has the characteristics of low cost, higher luminous efficacy, excellent heat dissipation potential and capability of prolonging the service life of the spotlight.

The invention discloses an enzyme linked immunosorbent assay kit for detecting sever fever with thrombocytopenia syndrome virus antigen. The enzyme linked immunosorbent assay kit comprises a reagent A, a reagent B, a reagent C, a reagent D, a reagent E, a reagent F, and a reagent H. The reagent A is an ELISA coated plate coated with a capture antibody; the reagent B is a PBS buffer solution; the reagent C is an antigen NP solution with the concentration of 15.6 ng/mL; the reagent D is an AuNP-Ab2-HRP probe concentrated solution with the gold nanoparticle concentration being 100 microgram/mL; for the reagent E, a 0.01 M PBS buffer solution containing 5% of BSA and having a pH value of 7.4 is used as a probe diluent; the reagent F is skim milk powder; the reagent G is 3, 3', 5, 5'-tetramethyl benzidine; and the reagent H is 0.1 M of HCL. The kit is high in sensitivity and specificity, and the aggregation concentration of enzymes on each immune sandwich structure is enhanced. The absorbance value under a specific wavelength is measured, and the sever fever with thrombocytopenia syndrome virus antigen NP can be qualitatively and quantitatively detected.

The invention relates to the technical field of LED packaging, and relates to a thermal insulation layer reflection type quantum dot LED packaging device and a lamp. The heat insulation layer reflection type quantum dot LED packaging device includes a substrate and an LED chip arranged on the substrate. The top of the LED chip is sequentially provided with Fluorescent coating, heat-insulating adhesive layer, quantum dot layer and barrier water and oxygen layer, a heat-insulating adhesive layer is arranged between the quantum dot layer and the fluorescent coating, in order to protect the quantum dots, a layer is provided between the quantum dot layer and the fluorescent coating There is a heat-insulating adhesive layer, and at the same time, a reflective material for reflecting light is arranged inside the heat-insulating adhesive layer. The reflection of the reflective material can improve the light-emitting angle of the LED chip, so that the light can be emitted at a larger angle. The reflection of the reflective material can Disperse the central beam of light to make the distribution of light more uniform and improve the lighting effect; the lamp includes a lamp holder, a lamp pole, a lamp cap and a thermal insulation layer reflective quantum dot LED packaging device. Compared with the prior art, the lamp has the above advantages.

The invention relates to a tea catechin histocyte chemical positioning method, which can solves the problems of the present phenols tissue chemical positioning technique that: bad coloration specificity, slow coloration speed, not high sensitivity, incapability of tea catechin histocyte chemical positioning. The tea catechin histocyte chemical positioning method comprises the following steps: taking the ender tea fresh leaves or tender stalk or root tip or tea callus tissue; producing the slice; dropping 1%-3%(w/v)vanillin concentrated hydrochloric acid solution at one side of the tissue slice and sucking the solution at another side of the tissue slice using filter paper until the material is fully reddened; sucking the redundant vanillin concentrated hydrochloric acid solution using filter paper and drying the slice surface using a blower to obtain a colored slice; taking photomicrograph of the colored slice in 30 minutes after coloration and recording the shape of the colored slice. The coloration process is simple and the operation is convenient. Compared with the typic phenols substance such as ferric chloride, the vanillin acid solution has features of high coloration speed, high sensitivity, good specificity; not only the catechine in the tea organ tissue is positioned and but also the catechine in tea organelle is accurately positioned.