2results about How to "Fast color development" patented technology

The invention discloses a method for accelerating the color development speed of filamentous fungus resazurin and application of the method, and relates to the technical field of drug sensitivity detection. The invention provides a method for accelerating the color development speed of filamentous fungus resazurin, which comprises the following steps: S1, diluting a filamentous fungus bacterial liquid by using an MUELLER-HINTON culture medium, and adding the diluted filamentous fungus bacterial liquid into a to-be-detected drug hole for incubation; s2, performing suction filtration after incubation; s3, adding a color developing agent containing a catalyst for color development; s4, result interpretation; the catalyst in the step S3 is menadione. According to the MUELLER-HINTON culture medium, in the Aspergillus drug sensitivity detection process based on resazurin color development, after a catalyst is added, color development is accelerated, meanwhile, all medicine adding holes cannot be red, and the MIC value can be accurately obtained.

This application relates to the field of in vitro bioassay technology, and discloses a method for preparing a colloidal gold assay reagent, its product, and its application. The method for preparing the colloidal gold assay reagent comprises five steps: colloidal gold preparation, antibody labeling, double blocking, centrifugal purification, and dilution and reconstitution. In particular, the double blocking process involves a first blocking agent selected from one or more of bovine serum albumin, ovalbumin, and casein; and a second blocking agent selected from one or more of lysine, glycine, alanine, serine, threonine, valine, and asparagine. This allows the prepared colloidal gold assay reagent to achieve high sensitivity and excellent C / T line color development when applied to FSH detection, reducing false positives caused by nonspecific adsorption and improving the accuracy of test results. Its limit of detection (LOD) is as low as 10 mIU / mL, solving the problems of low sensitivity and uneven C / T line color development in existing FSH assays.