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Isoelectric focusing electrophoresis isoelectric point detection method of recombinant protein

A technology of isoelectric focusing electrophoresis and recombinant protein, applied in the direction of measuring devices, material analysis through electromagnetic means, instruments, etc., to achieve scientific and reasonable results and effectively complete experiments

Inactive Publication Date: 2019-12-10
DONGGUAN TAILI BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The object of the present invention is to provide a method for detecting the isoelectric point of recombinant protein by isoelectric focusing electrophoresis in order to solve the problem of detecting the isoelectric point of recombinant protein by isoelectric focusing electrophoresis

Method used

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  • Isoelectric focusing electrophoresis isoelectric point detection method of recombinant protein
  • Isoelectric focusing electrophoresis isoelectric point detection method of recombinant protein
  • Isoelectric focusing electrophoresis isoelectric point detection method of recombinant protein

Examples

Experimental program
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Embodiment 1

[0065] Embodiment one, with reference to Figure 1~2 , a kind of isoelectric focusing electrophoresis isoelectric point detection method of recombinant protein, comprises the steps:

[0066] S1. Material preparation: Prepare all kinds of materials used in the experiment to ensure the smooth progress of the experiment;

[0067] S2. Reagent preparation: The reagents required for the experiment, the sample to be tested and the reference substance must be prepared in time for the experiment;

[0068] S3. Instrument preparation: the experimental instruments are cleaned in advance and prepared;

[0069] S4. Preparation of reagents: artificially prepare reagents that cannot be directly used in the experiment for use to ensure the smooth progress of the experiment;

[0070] S5. Inspection method: mainly use electrophoresis as a method to inspect it;

[0071] S6. Analyzing and judging results: analyzing and judging the results by comparing with reference substances;

[0072] Wherei...

Embodiment 2

[0077] Embodiment two, refer to Figure 1~3 , a kind of isoelectric focusing electrophoresis isoelectric point detection method of recombinant protein, comprises the steps:

[0078]S1. Material preparation: Prepare all kinds of materials used in the experiment to ensure the smooth progress of the experiment;

[0079] S2. Reagent preparation: The reagents required for the experiment, the sample to be tested and the reference substance must be prepared in time for the experiment;

[0080] S3. Instrument preparation: the experimental instruments are cleaned in advance and prepared;

[0081] S4. Preparation of reagents: artificially prepare reagents that cannot be directly used in the experiment for use to ensure the smooth progress of the experiment;

[0082] S5. Inspection method: mainly use electrophoresis as a method to inspect it;

[0083] S6. Analyzing and judging results: analyzing and judging the results by comparing with reference substances;

[0084] Wherein, the pre...

Embodiment 3

[0092] Embodiment three, refer to Figure 1~4 , a kind of isoelectric focusing electrophoresis isoelectric point detection method of recombinant protein, comprises the steps:

[0093] S1. Material preparation: Prepare all kinds of materials used in the experiment to ensure the smooth progress of the experiment;

[0094] S2. Reagent preparation: The reagents required for the experiment, the sample to be tested and the reference substance must be prepared in time for the experiment;

[0095] S3. Instrument preparation: the experimental instruments are cleaned in advance and prepared;

[0096] S4. Preparation of reagents: artificially prepare reagents that cannot be directly used in the experiment for use to ensure the smooth progress of the experiment;

[0097] S5. Inspection method: mainly use electrophoresis as a method to inspect it;

[0098] S6. Analyzing and judging results: analyzing and judging the results by comparing with reference substances;

[0099] Wherein, the ...

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Abstract

The invention discloses an isoelectric focusing electrophoresis isoelectric point detection method for recombinant protein. The isoelectric focusing electrophoresis isoelectric point detection methodcomprises the following steps: s1, material preparation, various materials used in the experiment are prepared properly so as to ensure that the experiment is carried out smoothly; s2, reagent preparation; reagents, to-be-detected samples and reference substances required by the experiment are required to be prepared in time for the experiment; s3, instrument preparation; experimental instrumentsare cleaned in advance and well reserved; s4, reagent preparation; reagents which cannot be directly used in an experiment are manually prepared for use, so that the experiment can be smoothly carriedout; s5, an inspection method; electrophoresis is mainly used as a method for inspection; s6, judgment result analysis, a result is analyzed and judged through a comparison reference substance; The method covers preparation of materials, reagents and instruments, reasonable preparation of the reagents, a scientific inspection method and rigorous analysis and judgment, isoelectric focusing electrophoresis isoelectric point detection is carried out on the recombinant protein, the result is scientific and reasonable, and the method is persuasive.

Description

technical field [0001] The invention belongs to the technical field of isoelectric point detection of recombinant protein by isoelectric focusing electrophoresis, and specifically relates to a method for detecting isoelectric point of recombinant protein by isoelectric focusing electrophoresis. Background technique [0002] Isoelectric focusing (Isoelectric focμsing, referred to as IEF) is a new technology that appeared in the mid-1960s. In recent years, new progress has been made in isoelectric focusing technology, and it has rapidly developed into a mature modern biochemical experiment technology. Because of its high resolution, good repeatability, large sample capacity, easy and fast operation, it has been widely used in biochemistry, molecular biology and clinical medicine research. [0003] Therefore, we developed the isoelectric focusing electrophoresis method of SMBS-4 on the basis of the second method of isoelectric focusing electrophoresis in the third volume of "C...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447
CPCG01N27/44795
Inventor 兰万军程玉陈静山张桂涛林如新
Owner DONGGUAN TAILI BIOTECH
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