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Method for restoring antibacterial activity of tigecycline

A technology of tigecycline and antibacterial activity, applied in the field of biology and medicine, can solve the problems of false drug resistance and achieve the effect of restoring sensitivity, accurate results and good repeatability

Active Publication Date: 2018-11-13
AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In order to solve the above-mentioned drug sensitivity problem, the inventor of the present application intends to provide a method for recovering the antibacterial activity of tigecycline by conducting research on various factors affecting the drug sensitivity test of tigecycline. Tigecycline Resensitization Liquid will help to solve the phenomenon of pseudo-resistance to tigecycline and provide a strong basis for the rational use of antibacterial drugs in clinical practice

Method used

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  • Method for restoring antibacterial activity of tigecycline
  • Method for restoring antibacterial activity of tigecycline

Examples

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Embodiment 1

[0036] Example 1: Disk method drug susceptibility test

[0037] According to the requirements of the American Association for Clinical and Laboratory Standardization, the standard disc diffusion method drug susceptibility test was carried out, and a suspension of Klebsiella pneumoniae 0.5 turbidites was evenly spread on the Mueller-Hinton agar plate, and dried for 15 minutes. Then paste the tigecycline and amikacin drug sensitive paper respectively, and measure the diameter of the inhibition zone after overnight incubation. After adding different concentrations of resensitization solution (the resensitization solution is EDTA solution, the concentration range is 100-400μg / sheet) (No. 2-5 paper), the inhibition zone expands, such as the inhibition zone of No. 4 tigecycline is 22mm; in order to prove that the resensitization solution itself has no inhibitory effect on bacteria, two amikacin paper sheets (No. 6 and No. 7) were pasted in this experiment. No. 6 paper was added with...

Embodiment 2

[0038] Example 2: Drug susceptibility test by agar dilution method

[0039] According to the requirements of the American Association for Clinical and Laboratory Standardization, the standard agar dilution method drug susceptibility test was carried out. The suspension of 18 strains of Klebsiella pneumoniae 0.5 M. turbidis was diluted 10 times and then inoculated in a series of with and without resensitization solution. On the MHA agar plate of tigecycline concentration, the drug susceptibility test results showed that in the presence of the resensitization solution, 9 of the 18 bacteria recovered their sensitivity to tigecycline, and their MIC values ​​ranged from > 8 μg / g / mL down to ≤1 μg / mL (eg figure 2 shown).

Embodiment 3

[0040] Example 3: Micro-broth dilution method drug susceptibility test

[0041] According to the requirements of the American Association for Clinical and Laboratory Standardization, the standard micro-broth dilution method drug susceptibility test was carried out, and a Klebsiella pneumoniae 0.5 turbidity bacteria suspension was diluted 100 times and then inoculated with and without resensitization solution In a series of tigecycline concentration microplates, the drug susceptibility test results showed that in the presence of resensitization solution, the bacteria recovered their sensitivity to tigecycline, and the MIC value decreased from >16 μg / mL to 1 μg / mL. mL (e.g. image 3 shown).

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Abstract

The invention belongs to the technical field of biology and medicine, and relates to drug susceptibility tests for important antibacterial agents, in particular to a method for restoring the antibacterial activity of tigecycline, aiming to achieve accuracy of tigecycline susceptibility results detected by in-vitro conventional drug susceptibility tests. The method has the advantages that a stabilizing agent is applied to eliminate influence factors in the tigecycline susceptibility tests, the tigecycline restores the susceptibility to pathogenic bacteria, and a simple method for accurately measuring the susceptibility of the tigecycline to the pathogenic bacteria is created; addition of other reagents is not needed, an appropriate volume of resensitization liquid is added into tigecyclinepaper sheets, MHA agar plates or microporous plates on the basis of normal operations, and accordingly, major problems in the tigecycline susceptibility tests in the prior art, such as false drug resistance, can be solved; the method is simple and convenient to operate, has accurate results and high repeatability, and the like.

Description

technical field [0001] The invention belongs to the fields of biology and medicine, and relates to a drug susceptibility test method for important antibacterial drugs, in particular to a method for restoring the antibacterial activity of tigecycline. The method is used for the drug susceptibility test against tigecycline and its influence Factors restore the antibacterial activity of tigecycline. Background technique [0002] The data show that due to the irrational use and abuse of antibacterial drugs, the rate of bacterial resistance to them has risen rapidly. According to the 2015 CHINET China bacterial drug resistance monitoring results, since 2005, the drug resistance rate of clinically isolated Gram-negative bacilli to important antibacterial drugs, especially carbapenems, has increased rapidly; The average resistance rate of isolated Klebsiella pneumoniae to carbapenems is 15%, and it can be as high as 50% in some hospitals; the average resistance rate of Acinetobact...

Claims

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Application Information

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IPC IPC(8): C12Q1/18
CPCC12Q1/18
Inventor 胡付品杨洋董栋郑永贵吴湜郭燕朱德妹
Owner AFFILIATED HUSN HOSPITAL OF FUDAN UNIV
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