Korean pseudomonas and application thereof

A technology of Pseudomonas and bacterial strains, applied in the field of microbiology and biological control, can solve the problems of authenticity distortion, production area relocation, production area and scale reduction, etc., and achieve the effect of good effect and strong cellulose degradation ability

Inactive Publication Date: 2016-05-18
FUJIAN AGRI & FORESTRY UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the cultivation and planting process of Pseudostellariae Pseudostellaria, there are serious continuous cropping obstacles. Continuous cropping Pseudostellariae Pseudostellariae plants do not grow well, and the underground part cannot expand normally (such as figure 1 As shown), after harvesting, it must be replanted after 2~4 years
The problem of continuous cropping obstacles has also led to a series of downstream unfavorable problems. For example, the production area and scale of Taizisandao are shrinking year by year, and even the production area has moved out, and authenticity is distorted.

Method used

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  • Korean pseudomonas and application thereof
  • Korean pseudomonas and application thereof
  • Korean pseudomonas and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Screening and identification of antagonistic bacteria Pseudomonas koreansis (Pseudomonaskoreensis) CJ-361

[0022] 1. Screening of antagonistic bacteria Pseudomonas koreensis CJ-361

[0023] The antagonistic bacteria Pseudomonas koreensis (Pseudomonas koreensis) CJ-361 described in the present invention is screened from rhizosphere soil of continuous cropping heterophylla.

[0024] 1.1 Pseudomonas medium preparation

[0025] The preparation method of pseudomonas selective isolation agar (PSIA) is as follows: Weigh 20 g of soybean-casein digest agar (soybean case indigestagar, SCD) (BD, USA), add 495.5 mL of double distilled water, heat and stir, fully After dissolving, add 1mL of 0.1% (wt / vol) crystal violet stock solution, sterilize at 121°C for 15 minutes under high temperature and high pressure, and when cooled to about 50°C, add 3.5mL of 5% (wt / vol) to the medium After fully mixing the furanidine stock solution, pour it quickly, cool and solidify to prep...

Embodiment 2

[0037] Antibacterial effect detection of embodiment 2 antagonistic bacteria

[0038] Use PDA medium, inoculate activated Pseudomonas korean CJ-361 at a distance of 2.5 cm from the center of the circle, place it in a constant temperature incubator at 28°C for 48 hours in the dark, and inoculate pathogenic fungi (such as Fusarium oxysporum and Fusarium moniliforme) were placed in a constant temperature incubator at 28°C in the dark for several days, and the formation of the inhibition zone was observed in real time. After culturing for 5 days, it was found that Pseudomonas koreensis CJ-361 could efficiently antagonize the mycelial growth of Pseudostellaria specialized type Fusarium oxysporum and Fusarium moniliforme (such as image 3 shown).

Embodiment 3

[0039] Example 3 Optimization of Optimal Culture Medium for Pseudomonas Korea (Pseudomonaskoreensis CJ-361)

[0040] Prepare the following medium to optimize the optimal growth medium for Pseudomonas koreensis CJ-361. The medium is as follows: 1) full LB solution; 2) 1 / 4LB solution; 3) 1 / 4LB+1 / 20MS ;4) 1 / 4LB+1 / 40MS; 5) 1 / 4LB+1 / 80MS; 6) 1 / 4LB+1 / 20MS+0.1wt.% brown sugar; 7) 1 / 4LB+1 / 40MS+0.1wt. % brown sugar; 8) 1 / 4LB+1 / 80MS+0.1wt.% brown sugar.

[0041] Among them, the formula of MS medium (no iron salts) is: 1) macroelements: 1900mg / LKNO 3 , 1650mg / LNH 4 NO 3 , 370mg / LMgSO 4 ·7H 2 O. 170mg / LKH 2 PO 4 , 440mg / LCaCl 2 2H 2 O; 2) Trace elements: 22.3mg / LMnSO 4 4H 2 O, 8.6mg / LZnSO 4 ·7H 2 O, 6.2mg / LH 3 BO 3 , 0.83mg / LKI, 0.25mg / LNa 2 MoO 4 ·7H 2 O, 0.025mg / LCuSO 4 ·5H 2 O, 0.025mg / LCoCl, 2.6mg / LH 2 O; 3) Organic matter: 2.0mg / L glycine, 0.5mg / L pyridoxine hydrochloride, 0.1mg / L ammonium sulfate hydrochloride, 0.5mg / L niacin, 100mg / L creatine; LB medium formula ...

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PUM

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Abstract

The invention provides a Korean pseudomonas and application thereof. The Korean pseudomonas is identified as Korean pseudomonas CJ-361, and the preservation number is CGMCC NO.12122. The Korean pseudomonas CJ-361 is obtained through separation from radix pseudostellariae rhizosphere soil by adopting a pseudomonas selectivity culture medium, an antagonistic experiment is performed on fusarium oxysporum and fusarium moniliforme separated to the radix pseudostellariae root lesion portion through an agar plate opposite culture method, and it is found that the Korean pseudomonas has the strong antagonism on the fusarium oxysporum and the fusarium moniliforme. Meanwhile, the invention provides the optimum culture medium for antagonistic bacterium growth, the optimum formula is 1/4LB+1/20MS (containing no ferric salt)+0.1 wt% brown sugar, and the fermented liquor has the obvious antagonistic effect. The antagonistic strain and the microbial agent thereof can effectively prevent and treat radix pseudostellariae root rot diseases, and the environment-friendly, ecological and safe biological prevention and control potential strain is provided for overcoming or relieving replantation diseases of radix pseudostellariae.

Description

technical field [0001] The invention relates to a bacterial strain, in particular to a Korean pseudomonas strain and application thereof, which can be used to overcome or alleviate the problem of soil-borne diseases of continuous cropping heterophylla, and belongs to the technical field of microorganisms and biological control. Background technique [0002] Pseudostellaria heterophylla, also known as child ginseng, baby ginseng and rice ginseng, is a perennial herb of Caryophyllaceae. Its roots are used as medicine. The Ministry of Health has determined to include it in the "List of Chinese Herbal Medicines that Can Be Used in Health Foods". Pseudostellaria is mainly produced in Fujian, Guizhou, Anhui and other places, among which Zherong County in Fujian Province has the longest cultivation history and the best quality, and is known as the "Hometown of Chinese Pseudostellaria". During the cultivation and planting process of Pseudostellariae Pseudostellaria, there are serio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/38
CPCA01N63/00C12N1/20C12N1/205C12R2001/38
Inventor 陈军吴林坤林文雄林翰黄珊瑜吴艳红林生
Owner FUJIAN AGRI & FORESTRY UNIV
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