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Chlorella preservation method

A technology for chlorella and culture containers, which is applied in the field of chlorella preservation, can solve the problems of increased labor costs and pollution risks, degradation of good properties of algae species, and harsh storage conditions, so as to facilitate batch preservation, facilitate management and The effect of maintenance and long storage time

Inactive Publication Date: 2018-02-16
FUJIAN INST OF MICROBIOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The storage time of liquid preservation method and solid plate preservation method is often short (usually 1-2 months). Since the surface of the medium is easy to dry, it needs to be subcultured regularly, but frequent subculture not only increases the labor cost and the risk of contamination, but also easily Lead to the degradation or loss of good traits of algal species
Although the cryopreservation method has a long storage time, it requires expensive equipment, consumes a lot of energy, and the storage conditions are harsh, and antifreeze agents that are toxic to algae cells need to be added to the storage solution, so it is difficult to be widely used

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Take Chlorella minutissima as the test algae strain, add it to the Erlenmeyer flask containing 100mL of growth medium, illuminance 6000lx, 24h continuous light, temperature 26℃, shaking culture under the conditions of 150rpm, the color of the culture solution can be observed Gradually turn green to dark green, and enter the logarithmic growth phase after 5 days.

[0025] Take the algae liquid of Chlorella minutis in the logarithmic growth phase for microscopic examination, and after confirming that there is no miscellaneous bacteria, draw 5 μL of the algae liquid and drop it on the middle position of the agar plate medium in the corresponding well of the 96-well culture plate (the same 96-well plate Different wells of the culture plate can store different chlorella species at the same time, just mark it with a marking pen on the front or back culture plate corresponding to the well). Place the 96-well culture plate filled with agar plate medium on a shaker for 3 minutes...

Embodiment 2

[0035] Take Chlorella pyrenoidosa as the test algae strain, add it to the Erlenmeyer flask filled with 100mL growth medium, and shake it under the conditions of 4000lx illumination, 24h continuous illumination, temperature 22°C, and 180rpm, and the culture medium can be observed The color gradually turns green to dark green, and it enters the logarithmic growth phase after 7 days.

[0036]Take the algae liquid of Chlorella pyrenoidosa in the logarithmic growth phase for microscopic examination. After confirming that there is no miscellaneous bacteria, draw 5 μL of algae liquid and drop it on the middle position of the agar plate medium in the corresponding well of the 96-well culture plate (the same 96-well culture plate). Different wells of the well culture plate can preserve different chlorella species at the same time, and can be marked with a marking pen on the front or back culture plate corresponding to the well). Place the 96-well culture plate filled with agar plate me...

experiment example 3

[0046] The number of live algae cells of Chlorella microella preserved by the method of Example 1 and the biomass of algae cells in the activated algae liquid were used as indicators to evaluate the preservation effect of the preservation method of Example 1 on Chlorella microminis.

[0047] Under aseptic conditions, the Chlorella microella preserved for 3 months, 6 months, 9 months and 12 months by the preservation method of Example 1 was scraped, added to sterile water, and made into algae liquid, and the flat algae was used to The counting method was used to measure the density of living algae in the algae liquid, and three parallel control groups were set up for each experiment. Calculate the average value of the algal cell survival rate, and the determination method refers to "Industrial Microbiology Experimental Technology Manual" (China Light Industry Press, 1997). Adopt the preservation method of embodiment 1 to preserve Chlorella microella after 3 months, 6 months, 9 ...

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Abstract

The invention discloses a chlorella preservation method. The chlorella preservation method comprises the following steps: dropping chlorella which is in a logarithmic growth phase and has no impure bacteria into a culture container added with an agar plate culture medium; oscillating to disperse the chlorella; then putting the chlorella into an illumination incubator with the temperature of 22 DEGC to 26 DEG C and the illumination intensity of 4000lx to 6000lx and carrying out continuous illumination culture for 5d to 10d at 24h; then sealing by utilizing a sealing film, reversing and preserving under low-temperature and weak-illumination conditions. The preservation method disclosed by the invention has the characteristics of convenience for operation, low cost, uneasiness of causing pollution, good preservation effect, long preservation time and the like and can keep the bioactivity of the chlorella at maximum and the chlorella has a strong recovery capability.

Description

technical field [0001] The invention belongs to the technical field of microalgae preservation, and more specifically, the invention relates to a method for preserving chlorella. Background technique [0002] The genus Chlorella (Chlorella) is a class of universal unicellular green algae, belonging to Chlorophyta, Chlorophyceae, Chlorcoccales, and Chlorellaceae. Common types of chlorella in my country include Chlorella pyrenoidosa, Chlorella ellipsoida [0003] (Chlorella ellipsoidea), common chlorella (Chlorella vulgaris) and tiny chlorella (Chlorella minutissima), etc. The application value of chlorella is very high, and it has the reputation of "gold in green algae". Has been widely used in water purification, food additives, animal feed, and medical care and other fields. [0004] The preservation of single-cell algae species of Chlorella has always been an important factor restricting the normal supply and long-term preservation of algae species in production culture...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/12C12N1/04C12R1/89
CPCC12N1/04C12N1/12
Inventor 聂毅磊陈宏
Owner FUJIAN INST OF MICROBIOLOGY
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