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Bacillus cereus with electrogenesis characteristic and application thereof in microbiological fuel cell

A Bacillus cereus and fuel cell technology, applied in the biological field, to achieve good electricity production capacity, broad industrial application potential, and high electroactivity

Inactive Publication Date: 2012-04-04
NANJING UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

No reports have been found at home and abroad on the electricity production characteristics of Bacillus cereus and its application in microbial fuel cells

Method used

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  • Bacillus cereus with electrogenesis characteristic and application thereof in microbiological fuel cell
  • Bacillus cereus with electrogenesis characteristic and application thereof in microbiological fuel cell
  • Bacillus cereus with electrogenesis characteristic and application thereof in microbiological fuel cell

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: Screening of MFC-1 bacterial strain

[0020] In the natural wastewater on the campus of Nanjing University, take 500ml of natural wastewater and put it into the anode chamber of the microbial fuel cell. The microbial fuel cell device adopts an H-shaped double-chamber structure ( figure 1 ), consisting of two glass bottles, which are connected with a glass tube connected to the glass bottle, and DuPont’s Nafion 211 proton exchange membrane is used in the middle, and the two chambers are tightened and connected with clips. The electrodes are made of high-purity carbon paper. The wires are connected with copper wire, and the exposed part is stuck with glue. The external circuit is connected with a 1000Ω fixed resistor, and the potential difference between the two ends of the resistor is measured with a multimeter. The anode of the battery is fed with high-purity nitrogen to ensure anaerobic state, and the cathode uses potassium ferricyanide solution. Place ...

Embodiment 2

[0022] Embodiment 2: the cultivation of MFC-1 bacterial strain

[0023] Pick the MFC-1 strain cultured in LB medium, inoculate it into a 15mL centrifuge tube containing 5mL LB medium, at 37 ℃, 120 rpm, after culturing for 12 hours, add it with 1% inoculum to 250 ml to In a 500 ml Erlenmeyer flask of the following medium, the content of the medium per liter: 10g C 6 h 12 o 6 , 5g yeast extract, 10g NaHCO 3 , 8.5g NaH 2 PO 4 , 37°C, 120 rpm, after culturing for 12 hours, centrifuge under anaerobic environment to obtain the bacteria again, which is used for the study of microbial electricity production.

[0024]

Embodiment 3

[0025] Embodiment 3: the electricity production of MFC-1 bacterial strain

[0026]According to the culture and inoculation methods described in Example 2, cells in the late logarithmic growth period were obtained from 200mL medium with glucose as the electron donor, centrifuged under anaerobic conditions (8000rpm, 10min), washed three times with 50mM PBS buffer Re-dispersed into anaerobic medium with 50mM glucose as electron donor, inoculated into the anode of the microbial fuel cell. The anode chamber keeps anaerobic environment through high-purity nitrogen, and the catholyte is potassium ferricyanide solution of 0.1M PBS buffer solution as the electron acceptor. The battery device and operating conditions are as described in Example 1. The external resistance adopts 1000 ohms. The terminal voltage is recorded every 5 minutes with a multimeter, and the process of battery power generation is shown in image 3 shown.

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Abstract

The invention relates to a Bacillus cereus with electrogenesis characteristic, which is preserved in 'China General Microbiological Culture Collection Center' on 2011 July 12th, and the accession number is CGMCC No. 5055. The bacterial strain is identified as a Bacillus cereus bacterial strain, which has the following characteristics: a cell rod shaped thallus, a square end, short or long chains,a diameter of 0.4 to 0.6 micrometers, a bacterial length of 2.0 to 5.0 micrometers gram positivity, a large bacterium colony, rough, flat and irregular surface. On the plain agar plate, at a temperature of 37 DEG C for 24 hours cultivation, the Bacillus cereus can form a round or similar round, soft-texture, non-pigmented, lightly glossy, white bacterial colony (with a candle-like color) with a diameter of 5 to 7 millimeters, and can use a plurality of carbon sources and grow under anaerobic and aerobic conditions. A microbiological fuel cell produced by using the bacterial strain has higher electrogenesis capability and the Bacillus cereus can not only be directly used to produce electricity in microbiological fuel cell but also utilize sewage organic matter to produce electricity.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to the electricity production characteristics of a bacillus, ie, Bacillus cereus, and its application research in microbial fuel cells. Background technique [0002] Energy shortage and environmental pollution are two major challenges facing the sustainable development of our modern society. With the continuous development of society and economy, the massive consumption of energy leads to serious deterioration of environmental quality, especially the greenhouse effect caused by the increase of carbon emissions will have a long-term impact on the global climate. Therefore, achieving the unification of environmental benefits, economic benefits and social benefits by saving energy or realizing the substitution of new energy sources is the most important research topic at present. Microbial Fuel Cell (MFC) is a new and convenient energy device. It can use various organic matte...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20H01M8/16C12R1/085
CPCY02E60/527Y02E60/50
Inventor 张剑荣赵翠娥郭利川刘常宏
Owner NANJING UNIV
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