Method for preparing rhamnolipid

Abstract

The invention discloses a manufacture method for rhamnolipid that includes the following steps: filtering fungus, inoculating into culture medium after taking dipping preprocess to the sample getting from oil field sewage or grease, inoculating on blood agar plate for culturing, selecting single colony, inoculating on blue agar plate for culturing, selecting the colony having blue halo to the bevel for microbial conserving; fermenting the primary screening strain in fermenting culture medium, testing the oil drainage activity, emulsibility and fermenting liquid surface tension, selecting target strain; taking ferment to the selected strain, and optimizing the fermenting culture medium, fermenting condition and strain to gain rhamnolipid fermenting liquid; distilling rhanmolipid raw product from the rhamnolipid fermenting liquid. The invention has high yield, good heavy hydrocarbon degradation effect, and improves the selecting accuracy.

Description

1. Technical field [0001] The invention discloses a preparation method of glycolipid biosurfactant, particularly a preparation method of rhamnolipid. 2. Background technology [0002] Rhamnolipid belongs to a glycolipid biosurfactant. In addition to reducing surface tension, stabilizing emulsion and increasing foam, it also has non-toxic and biodegradable properties that general chemical synthetic surfactants do not have. advantage. Therefore, it can be widely used in various fields of industry, agriculture, medicine and daily life, especially in the fields of petroleum industry and environmental engineering, such as biological viscosity reduction of petroleum, enhanced oil recovery, bioremediation of heavy oil polluted soil, etc. In addition, as a natural additive, rhamnolipid is more and more popular in the food industry, fine chemical industry, medicine and agriculture. [0003] In the prior art, there have been reports of synthesizing rhamnolipids by microbial methods....

AI Technical Summary

Problems solved by technology

There are following deficiencies in the above-mentioned method for preparing rhamnolipids: 1, adopt the dilution coating method of blood agar plate to carry out the screening of producing rhamnolipid bacterial strains, although the generation of hemolytic circle can be seen visually, but hemolytic circle is irregular, Some components or miscellaneous bacteria in the blood often produce similar hemolytic circles. In actual operation, it is difficult to judge the amount of surfactant produced by the size of the hemolytic circle, and during the culture process, some components or miscellaneous bacteria in the blood often It will also produce similar hemolytic circles, which will cause bacterial contamination during the preparation and use of the medium; 2. This screening method has a large workload when it is diluted and coated, and the growth of the bacterial strain is slow when crude oil is the only carbon source, thus causing The screening cycle is longer; 3. This method needs to use different carbon sources in the fermentation process, that is, feed feeding is required, so the operation process is increased, and the yield of the strain is still too low, which is not conducive to industrial application

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