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A method of simply and rapidly preparing a bdellovibrio bacteriovorus ecological preparation

A technology for phagocytosing leech vibrio and leech vibrio, applied in the field of microorganisms, can solve problems such as unreported, does not affect the predation activity of leech vibrio, and achieves the effect of saving time and material, avoiding biological hazards and simple operation.

Inactive Publication Date: 2017-06-30
THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, there are many methods for isolating Bdellovibrio, but a method that is simple and does not affect the predation activity of Bdellovibrio has not been reported. After repeated experiments by the inventor, a simple and rapid preparation of Bdellovibrio is now reported. The ecoformulation approach

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] Example 1: Rapid preparation of bacteriophage Bdellovibrio ecological preparation isolated from pond sludge

[0014] 1. Sample collection:

[0015] The pond sludge was collected as a strain source for Bdellovibrio isolates.

[0016] 2. Preparation of host bacterial suspension:

[0017] The host bacteria EC600 was inoculated in LB liquid medium, shaken and cultured at 35°C and 180rpm for 18h, and then centrifuged at 8000r / min for 10min, and the precipitate was resuspended with sterile tap water to become the host bacteria suspension, and was used without Bacteria tap water to adjust the concentration to 10 10 cfu / mL, stored at 4°C for later use.

[0018] 3. Isolation of Bdellovibrio:

[0019] (1) Add 200 mL of sterile tap water to the obtained sludge sample, shake and mix well, take the supernatant and centrifuge at 500×g for 10 min, and store the obtained supernatant water sample at 4°C for later use;

[0020] (2) Take 50mL water sample and add it to 200mL host bac...

Embodiment 2

[0025] Example two: rapid preparation of bacteriophage Bdellovibrio ecological preparations from river silt

[0026] The difference from Example 1 is that river sludge was collected in Step 1, and used as the strain source of Bdellovibrio isolates, which were obtained by isolation according to the method of Example 1.

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PUM

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Abstract

An improved method of preparing a bdellovibrio bacteriovorus preparation is disclosed. Through repeated researching and experiments by inventors, a bdellovibrio bacteriovorus plaque obtained by screening adopting a two-layer tap water agar plate is picked and added into 200 mL of a host bacterium suspension, then the mixture is subjected to shake cultivation at 28 DEG C for 90 h, with a speed being 150 rpm, and an obtained liquid mixture is adopted as a bdellovibrio bacteriovorus suspension. The bdellovibrio bacteriovorus suspension can be directly used for tests. The whole preparing process is simple and rapid, thus saving time and the cost.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a preparation method of bacteriophage Bdellovibrio. Background technique [0002] Bdellovibrio bacteriovorus is a small, short rod-shaped or arc-shaped gram-negative bacteria with extremely long flagella at one end and extremely active movement. It is widely distributed in soil, seawater, sewage, sediments, etc. It can be detected even in mammalian feces, and it has the biological characteristics of parasitizing and lysing host bacteria. It is smaller than normal bacteria and thus able to invade and lyse host cells. Experiments have shown that the ability of Bdellovibrio to lyse pathogenic bacteria is significantly greater than that of non-pathogenic bacteria, and intestinal pathogenic bacteria are more easily lysed. In addition, Bdellovibrio bacteriophage can also play a certain role in water purification. In recent years, with the development of aquacultur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/01
CPCC12N1/20C12N1/205C12R2001/01
Inventor 周铁丽孙瑶曹建明
Owner THE FIRST AFFILIATED HOSPITAL OF WENZHOU MEDICAL UNIV
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