Vacuum thin film filtration and microbial cultivation device
A technology of microbial culture and vacuum film, which is applied in the cultivation devices of tissue cells/viruses, biochemical equipment and methods, biochemical instruments, etc., can solve the problems of vacuum pump inflow damage noise, manual repeated dumping, and increased artificial pollution, etc. Increase filtration speed, reduce labor intensity, and protect the effect of microporous membrane
Inactive Publication Date: 2008-05-21
沈佳特
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Problems solved by technology
In general, the volume of the suction filter bottle is between 250ml and 2000ml, and the single filtration volume is limited
When the filtration volume exceeds the volume limit, it is necessary to manually dump the filtrate repeatedly to complete the entire treatment process; after filtration, it is necessary to manually clean and disinfect the suction filter bottle; Porous film, which increases the chance of artificial contamination; when cultivating microorganisms with film, it is necessary to prepare agar plate; at the same time, there are problems such as the vacuum pump is easily damaged by the liquid and the noise is loud
In short, common assembled vacuum filter devices have major defects and are not convenient to use
Method used
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[0066] 1. Machine installation
[0067] As shown in Figure 1, Figure 2, and Figure 4, the membrane filter (1), filtrate receiving container (2), electric diaphragm pump (3), and drain pipe (11) are connected in sequence to form a filtered liquid flow path for sample filtration;
[0068] As shown in Figure 1, the ozone generator (6) and the pressure sensor (7) are respectively connected to the filtrate receiving container (2) through the two air inlets of the air inlet solenoid valve (4) to form an ozone gas flow path, so that the filtrate receiving container (2) Obtain ozone when disinfection is required, and monitor the vacuum degree in the filtrate receiving container (2);
[0069] As shown in Figure 1, the solvent bottle (10), liquid inlet solenoid valve (5), and filtrate receiving container (2) are connected in sequence to form a cleaning liquid flow path, so that the filtrate receiving container (2) can obtain solvent when cleaning is required;
[0070] As shown in Fig. ...
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The invention discloses a vacuum membrane filtering and microorganism culturing device, which relates to an analyzing filtration and microorganism culture device. The invention applies an electric diaphragm pump as the vacuum source; the liquid medium is absorbed on the cellulose liner which is positioned below the micro-porous membrane through the vacuum membrane filtering method; the membrane filtering device is refitted into a culture dish; and the device is cleaned and sterilized automatically with the cleansing detergent and ozone. The device is a mechanical and electrical integration device, which comprises the membrane filtering device (1), a filtrate receiver (2), the electric diaphragm pump (3), an air-in electromagnetic valve (4), a liquid-in electromagnetic valve (5), an ozone generator (6), a pressure sensor (7), a liquid crystal display (8), a micro-control system (9), a solution bottle (10) and a drain pipe (11). The invention has convenient application and strong practicality; the invention avoids the busy work of dumping filtrate, transferring membrane, cleaning and sterilizing and preparing agar plate; the invention also has the characteristics of intelligence, integration and multi-function; the invention suits for filtration technology areas such as medicine and health etc.
Description
technical field [0001] The invention relates to a device for analyzing, filtering and cultivating microorganisms; in particular, it relates to a device for vacuum membrane filtering and cultivating microorganisms by sticking film. Background technique [0002] Vacuum negative pressure filtration is a very useful and practical technology during the filtration treatment of liquid samples and the isolation and cultivation of microorganisms. The common vacuum negative pressure filter devices are mostly assembled, that is, the vacuum pump, the filter bottle and the filter are connected by conduits, etc., and the structure is simple. In general, the volume of the suction filter bottle is between 250ml and 2000ml, and the single filtration volume is limited. When the filtration volume exceeds the volume limit, it is necessary to manually dump the filtrate repeatedly to complete the entire treatment process; after filtration, it is necessary to manually clean and disinfect the suct...
Claims
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IPC IPC(8): C12M3/06
Inventor 沈佳特
Owner 沈佳特
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