Method for detecting anti-mold performance of silkworm excrement product

Abstract

The invention discloses a method for detecting anti-mold performance of a silkworm excrement product. The method comprises a first step of performing streak inoculation on a nutrient agar bevel, so as to serve as bevel preservation fungi; a second step of pouring sterile water into bevel strains cultivated well in the first step, washing spore liquid and pouring the spore liquid into a sterile triangular flask containing glass beads, and obtaining fungal spore liquid; a third step of centrifuging the fungal spore liquid, removing supernatant, adding sterile water to perform centrifugal washing, and preparing inoculating fungal spore liquid; a fourth step of manufacturing sample adding bags; a fifth step of adding the sample adding bags into sterile water to undergo pre-wetting to serve as blank reference samples, respectively adding anti-bacterial and non-anti-bacterial silkworm excrement products into the sample adding bags, adding sterile water for pre-wetting to serve as a to-be-tested sample and a contrast sample; a sixth step of respectively placing the to-be-tested sample, the contrast sample and a blank contrast sample on the surfaces of agar plates coated with the inoculating fungal spore liquid, and taking and adding the inoculating fungal spore liquid dropwise to the three samples; and a seventh step of recording the anti-mold performance of the silkworm excrement product. The method is simple to operate, quick in speed and low in using cost.

Description

technical field [0001] The invention relates to the anti-mold performance of silkworm excrement products, in particular to a method for detecting the anti-mold performance of silkworm excrement products with simple operation, high speed and low use cost. Background technique [0002] Silkworm excrement, also known as silkworm arrow, is mostly produced in Jiangsu, Zhejiang, Sichuan and other places. It is the dry excrement of silkworm moth larvae. Sweet and warm in nature and flavor, it has the effects of drying dampness, expelling wind, transforming turbidity, promoting blood circulation and relieving pain. Folks use silkworm excrement as pillow filling, which has the effects of clearing the liver and eyesight, nourishing the eyes, nourishing the brain, treating high blood pressure, and tinnitus, especially for infants and young children. The silkworm sand pillow is made of high-quality silkworm sand to keep the temperature of the head constant. It can really cool the head...

AI Technical Summary

Problems solved by technology

[0003] However, to carry out antibacterial testing on silkworm excrement products to judge their antibacterial performance and efficacy, there is currently no existing detection method that can be used, and ultimately it is impossible to implement the antibacterial performance evaluation of silkworm excrement products