Method for detecting anti-mold performance of silkworm excrement product

A technology of anti-mold and products, which is applied in the field of testing the anti-mold performance of silkworm excrement products, can solve the problems that the antibacterial performance and evaluation of silkworm excrement products cannot be implemented, and achieve the effect of low cost, fast speed and simple operation

Pending Publication Date: 2016-10-26
SUZHOU VOCATIONAL UNIV
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Problems solved by technology

[0003] However, to carry out antibacterial testing on silkworm excrement products to judge their antibacterial performance and efficacy, there is currently no

Method used

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Embodiment Construction

[0017] The preferred embodiments of the present invention are described in detail below, so that the advantages and features of the present invention can be more easily understood by those skilled in the art, so as to define the protection scope of the present invention more clearly.

[0018] Embodiments of the invention include:

[0019] A method for detecting anti-mold properties of silkworm excrement products, the specific steps are as follows:

[0020] Step 1: Add culture medium to the mold freeze-dried bacteria powder to dissolve to obtain a bacterial suspension, use a pipette gun to inoculate a little bacterial suspension onto the slant of the medium, and incubate at 28°C±1°C for 7~14 days. Store at 2-8°C for 1 month as a slant-preserved fungus; in the test, fresh mold cultures transferred within 3-10 generations should be used.

[0021] Step 2: Pour sterile water containing 0.05% non-ionic dispersant into the cultured slant strains in step 1, use a sterile inoculation ...

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Abstract

The invention discloses a method for detecting anti-mold performance of a silkworm excrement product. The method comprises a first step of performing streak inoculation on a nutrient agar bevel, so as to serve as bevel preservation fungi; a second step of pouring sterile water into bevel strains cultivated well in the first step, washing spore liquid and pouring the spore liquid into a sterile triangular flask containing glass beads, and obtaining fungal spore liquid; a third step of centrifuging the fungal spore liquid, removing supernatant, adding sterile water to perform centrifugal washing, and preparing inoculating fungal spore liquid; a fourth step of manufacturing sample adding bags; a fifth step of adding the sample adding bags into sterile water to undergo pre-wetting to serve as blank reference samples, respectively adding anti-bacterial and non-anti-bacterial silkworm excrement products into the sample adding bags, adding sterile water for pre-wetting to serve as a to-be-tested sample and a contrast sample; a sixth step of respectively placing the to-be-tested sample, the contrast sample and a blank contrast sample on the surfaces of agar plates coated with the inoculating fungal spore liquid, and taking and adding the inoculating fungal spore liquid dropwise to the three samples; and a seventh step of recording the anti-mold performance of the silkworm excrement product. The method is simple to operate, quick in speed and low in using cost.

Description

technical field [0001] The invention relates to the anti-mold performance of silkworm excrement products, in particular to a method for detecting the anti-mold performance of silkworm excrement products with simple operation, high speed and low use cost. Background technique [0002] Silkworm excrement, also known as silkworm arrow, is mostly produced in Jiangsu, Zhejiang, Sichuan and other places. It is the dry excrement of silkworm moth larvae. Sweet and warm in nature and flavor, it has the effects of drying dampness, expelling wind, transforming turbidity, promoting blood circulation and relieving pain. Folks use silkworm excrement as pillow filling, which has the effects of clearing the liver and eyesight, nourishing the eyes, nourishing the brain, treating high blood pressure, and tinnitus, especially for infants and young children. The silkworm sand pillow is made of high-quality silkworm sand to keep the temperature of the head constant. It can really cool the head...

Claims

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Application Information

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IPC IPC(8): C12Q1/18C12R1/685C12R1/645C12R1/80C12R1/885
CPCC12Q1/18
Inventor 王毅婧董斌
Owner SUZHOU VOCATIONAL UNIV
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