Method of separating screening heterotrophic nitration bacteria

Abstract

The invention relates to a method of separating and scalping heterotrophy nitrobacteria. Its making steps are preparing beef extract-peptone liquid medium; heating sterilization after adding agar; condensing into flat solid medium; coating mixed microorganism flora to beef extract-peptone flat; setting in incubator at 30 centigrade degree to culture for 7-10 days; selecting mono bacteria to fall on the extract-peptone flat line to purify to from pure bacteria; inoculating the pure bacteria lawn to ammonia oxidation ability identification culture solution; dipping gelisi reagent into the culture solution to do nitration activity identification. The upper steps can separate heterotrophy nitrobacteria from the mixed microorganism series. So the invention is exact and reliable.

Description

Technical field [0001] The invention relates to a method for separating and screening heterotrophic nitrifying bacteria, in particular to a method for separating, screening and identifying heterotrophic bacteria with nitrifying activity from an artificially simulated urban sewage treatment system, and belongs to the technical field of microorganisms. Background technique [0002] In the natural environment, nitrification is mainly accomplished by the nitrification ability of microorganisms. The concept of nitrification currently mentioned generally refers to the process of oxidation of ammonia nitrogen to nitrite nitrogen, and then further oxidation to nitrate nitrogen. This process is completed by two different types of bacteria: one is ammonia oxidizing bacteria, which oxidize ammonia nitrogen to nitrite nitrogen; the other is nitrite oxidizing bacteria, which oxidize nitrite nitrogen to nitrate nitrogen. At present, it is generally believed that the main body of nitrification ...

AI Technical Summary

Problems solved by technology

Due to the complex components of peptone and beef extract currently available in the domestic market, if you use peptone that is more easily available on the market in order to reduce costs, often before the coating and streaking process in the experimental separation process, the surrounding colonies will be damaged due to cultivation. A certain amount of nitrite or nitrate will have a certain amount of nitrite or nitrate due to the influence of the base, which will inevitably have a great impact on the judgment of the experimental results, thereby affecting the separation results of heterotrophic nitrifying bacteria.