Separation method of melon powdery mildew

Abstract

The invention discloses a monospore separation method of a melon powdery mildew. The method comprises the following steps: collecting a diseased melon leaf; brushing old spores from the surface of the diseased melon leaf by using a sterilizing brush; maintaining the diseased melon leaf under the condition of heat and moisture preservation so as to be bred into new spores; blowing the new cultured conidiospores onto a water agar plate through an ear washing bulb; utilizing a specific picking needle to pick out a single conidiospore under an anatomical lens; inoculating the single conidiospore on the leaf disc of the sterilized diseased melon or cucumber product; horizontally putting the leaf disc in a culture dish containing a mannitol culture medium; placing the leaf disc in an illumination incubator after overlaying a culture dish cover; and culturing the leaf disc for 7 days, thereby obtaining a purified melon powdery mildew bacteria colony. According to the invention, the single conidiospore is picked out by using the specific picking needle and is germinated normally under the action of suitable culturing medium and under the condition of suitable culturing condition, so that the separated melon powdery mildew has a pure pathogen and is free of microspecies cross infection. Therefore, the accuracy and the reliability of a subsequent research result are ensured. As a result, the prepared melon powdery mildew has an important practical value.

Description

technical field [0001] The invention relates to the technical field of a method for isolating biological pathogenic bacteria, in particular, the invention relates to the technical field of a method for isolating powdery mildew of melon. Background technique [0002] Melon is a Cucurbitaceae ( Cucurbitaceae ) Melon ( Cucumis Linn ) is an annual vine herbaceous plant, which is widely cultivated at home and abroad and is an important economic crop. According to statistics, the sown area of ​​muskmelon in my country is about 153,000 hectares, and the total annual output is more than 3 million tons, ranking first in the world (Liu Junpu et al., 2000). Melon powdery mildew is one of the important diseases in the production of muskmelon. The muskmelon powdery mildew in the open and protected fields in our country causes a large amount of production reduction every year, and seriously affects the quality of the product. According to reports, melon powdery mildew is mainly caused b...

AI Technical Summary

Problems solved by technology

[0003] Melon powdery mildew is a worldwide disease that occurs widely in various melon producing areas in my country; the powdery mildew fungus mainly infects the leaves of muskmelon plants, and can occur in the seedling and adult stages of muskmelon plants; under suitable conditions , melon powdery mildew develops rapidly, and spreads to the entire melon field or greenhouse in a few days or a week, causing the leaves to turn yellow, lose function, and gradually die, seriously affecting the yield and quality of melon fruits

[0004] The race succession and complex differentiation of powdery mildew of melon are very difficult for race identification and related pathological research; the isolation and cultivation of purified powdery mildew is the prerequisite for pathological analysis and disease-resistant breeding of powdery mildew of melon. Yet up to now, the technology of separating and purifying powdery mildew of melon has not been established yet; Therefore, it is very necessary to provide a kind of separation method suitable for powdery mildew of melon