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Culture device for white fly and tetranychid mite feeding experiment and method for conducting experiment using same

A cultivation device and whitefly technology, applied in animal husbandry and other fields, can solve problems such as difficult to solve insect upward test error, difficult to control temperature and humidity, death of spider mites in water, etc., to achieve controllable range of insect activities, easy The effect of temperature control and long shelf life of leaves

Inactive Publication Date: 2011-06-29
INSPECTION & QUARANTINE TECH CENT OF GUANGDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, using this method to carry out feeding experiments has many shortcomings. First, because it is a living host plant, it is very affected by the size of the space, and it is difficult to control the temperature and humidity; large error between
The application of leaf cages is easy to cause damage to the leaves, which will lead to the flight of whiteflies; while it is difficult to solve the problem of experimental errors caused by insect upwards with insect-proof covers
Tetranychus generally use leaf feeding method or water isolation culture method. Leaf feeding method will easily cause spider mites to escape; water isolation culture method will easily cause spider mites to drown and die

Method used

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  • Culture device for white fly and tetranychid mite feeding experiment and method for conducting experiment using same
  • Culture device for white fly and tetranychid mite feeding experiment and method for conducting experiment using same
  • Culture device for white fly and tetranychid mite feeding experiment and method for conducting experiment using same

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] Such as figure 1 As shown, the culture device of the whitefly and spider mite feeding experiment of the present embodiment comprises a petri dish 1 with a water agar plate 2 in the petri dish, and the formula of the water agar is composed of agar and water according to the mass ratio of 1: 50 composition, also includes the plastic wrap 4 that can cover petri dish, has several ventilation holes 3 on the plastic wrap 4.

[0021] Use case:

[0022] 1. Eclosion experiment of whitefly pupae:

[0023] Add 2g of agar to 100ml of distilled water, after high-temperature sterilization, pour it into a sterilized petri dish, cover it after cooling, and put it in the refrigerator for later use.

[0024] The front side of the leaf with the older nymphs of whitefly was attached to the water agar plate, sealed with plastic wrap, and the air holes were pierced with a dissecting needle. During this period, depending on the water evaporation rate of the water agar in the petri dish, re...

Embodiment 2

[0035] Such as figure 2 As shown, the culture device of the whitefly and spider mite feeding experiment of the present embodiment comprises a petri dish 1, and a water agar plate 2 is arranged in the petri dish 1, and the formula of the water agar is composed of agar and water according to a mass ratio of 1 :50 composition, also includes a plastic cup 5, the culture dish 1 is buckled upside down on the cup mouth of the plastic cup 5, there are some ventilation holes 3 on the peripheral side of the plastic cup 5.

[0036] Use case:

[0037] 1. Indoor fecundity experiment:

[0038] Add 2g of agar to 100ml of distilled water, after high-temperature sterilization, pour it into a sterilized petri dish, cover it after cooling, and put it in the refrigerator for later use.

[0039] 1. Indoor fecundity experiment of Bemisia tabaci: Place the front of the fresh and clean leaves on the water agar plate, cut the leaves to the size of the petri dish, and place the petri dish with the w...

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Abstract

The invention discloses a culture device for a white fly and tetranychid mite feeding experiment and a method for conducting experiment using the same. The culture device comprises a culture dish and a sealing device covering the culture dish, wherein a water agar plate is arranged in the culture dish; the sealing device covering the culture dish is provided with multiple air holes; and the water agar comprises agar and water in a mass ratio of 1:50. When the culture device for the white fly and tetranychid mite feeding experiment is used to conduct experiment, the leave is separated and can be sheared into any shape without using the entire leave as the experimental material, thereby avoiding influence on the experiment caused by the limitation under space, temperature and humidity when the living plant or cut plant is used as the experimental material; and the experimental conditions are easy to control, and the operation is simple. Moreover, the problem of relatively large error between the repeated experiments for the same treatment caused by differences in height, size and strongness of the living plants or cut plants can be overcome.

Description

Technical field: [0001] The invention belongs to the field of insect cultivation, and in particular relates to a cultivation device for whitefly and spider mite feeding experiments, an indoor cultivation experiment using the same for breeding whitefly and spider mite, indoor fecundity and host selectivity experiment for whitefly and spider mite method. Background technique: [0002] At present, feeding experiments on whiteflies are carried out on live host plants, and leaf cages or insect-proof covers are used to isolate whiteflies and host plants from the external environment. However, using this method to carry out feeding experiments has many shortcomings. First, because it is a living host plant, it is very affected by the size of the space, and it is difficult to control the temperature and humidity; The error between is large. The application of the leaf cage is easy to cause damage to the leaves, which will lead to the flight of whiteflies; while it is difficult to ...

Claims

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Application Information

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IPC IPC(8): A01K67/033
Inventor 林莉胡学难梁帆
Owner INSPECTION & QUARANTINE TECH CENT OF GUANGDONG ENTRY EXIT INSPECTION & QUARANTINE BUREAU
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