Method for counting effective viable bacterium content of Bdellovibrio bacteriovorus powder

Abstract

The invention provides a method for counting the effective viable bacterium content of Bdellovibrio bacteriovorus powder, which comprises the following steps: appropriately diluting a sample to be detected, so that Bdellovibrio bacteriovorus in the sample to be detected is sufficiently dispersed into individual cells; then, carrying out incubation culture in an aseptic nutrient broth diluted by 10 times to promote growth and propagation, thus forming a megascopic turbid liquid; further determining that a large amount of Bdellovibrio bacteriovorus is contained in the turbid liquid having the maximum dilution through plaque detection; and finally, determining the total amount of effective viable bacteria in the sample to be detected. Compared with a microscopic direct observation method anda dilution-by-tap-water double-layer agar plate method, the method provided by the invention is more accurate, and overcomes the defects that detection is inaccurate due to dilution in the dilution-by-tap-water double-layer agar plate method and killed bacteria and viable bacteria can not be distinguished in the microscopic direct observation method.

Description

technical field [0001] The invention relates to a microorganism measurement technology, in particular to a method for counting the effective viable bacteria content of bacteriophage Bdellovibrio powder. Background technique [0002] Bdellovibrio phage preparation is a green product with no toxic side effects and no drug residues. It is rich in Bdellovibrio phage with unique phage characteristics, and is a commonly used water quality microbial improver in aquaculture. However, the lack of quality standards is one of the main problems in the development of bacteriophage Bdellovibrio preparations, and one of the biggest problems leading to the lack of quality standards is that the number of live bacteria does not match the label, and some live bacteria are even difficult to detect. Therefore, a stable and reliable counting method of Bdellovibrio preparations is undoubtedly of great significance to the normal production and quality control of Bdellovibrio preparations. At prese...

AI Technical Summary

Problems solved by technology

For example, the microscope direct counting method cannot distinguish dead bacteria from live bacteria, and the counting result is the sum of dead bacteria and live bacteria; the dilute tap water double-layer agar plate method continuously reduces the concentration of bacteriophage Bdellovibrio due to gradient dilution, Ultimately, low concentrations of Bdellovibrio bacteriophages cannot be detected, making the detection results inaccurate

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