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Method for preparing mixed liquor of glucose and fructose from bagasse

A sugarcane bagasse and glucose technology is applied in the field of sugarcane to achieve the effects of reducing production cost, simple process and mild culture conditions

Active Publication Date: 2014-11-05
THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no report on the use of microorganisms to degrade bagasse to obtain a mixture of glucose and fructose

Method used

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  • Method for preparing mixed liquor of glucose and fructose from bagasse
  • Method for preparing mixed liquor of glucose and fructose from bagasse
  • Method for preparing mixed liquor of glucose and fructose from bagasse

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] Embodiment 1 The method for preparing glucose and fructose mixed solution by enzymatic hydrolysis of bagasse

[0035] 1) After activating the strain Bacillus aryabhattai GZ03 with an activation medium plate (1% peptone, 0.2% yeast extract, 1.5% agar powder, prepared in situ seawater, sterilized at 121°C for 20 min), pick a single Colonies were inoculated into seed medium (1% peptone, 0.2% yeast extract, prepared in situ with seawater, sterilized at 121°C for 20min before use), and cultured with shaking at 200r / min for 12h at 28°C.

[0036] 2) Add the seed liquid to the sugar-producing medium (containing 2% of bagasse powder sieved by 60 meshes, prepared in situ seawater, and sterilized at 121° C. for 20 min) according to the inoculation ratio of 2%, and continue at 28° C. Under the condition of 200r / min shaking culture for 48h, the fermentation broth was obtained.

[0037] 3) Centrifuge the fermentation broth at 10,000 × g for 15 min, and collect the supernatant.

[0...

Embodiment 2

[0066] Similar to Example 1, the difference is that the cultivation temperature in steps 1) and 2) is 25°C, the content of yeast paste in the seed medium in step 1) is 0.5%, and the bagasse powder in step 2) was 2%, and the culture time was 72h.

Embodiment 3

[0068] Similar to Example 1, the difference is that the culture temperature in steps 1) and 2) is 37°C, the content of yeast paste in the seed medium in step 1) is 1.5%, and the bagasse powder in step 2) was 5%, and the incubation time was 96h.

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Abstract

The invention relates to sugarcane and provides a method for preparing mixed liquor of glucose and fructose from bagasse. The accession number of the bacterial strain Bacillus aryabhattai GZ03 is CCTCC No: M2013521. The method for preparing mixed liquor of glucose and fructose from bagasse comprises the following steps: 1) activating the bacterial strain Bacillus aryabhattai GZ03 with an activation medium plate, inoculating the bacterial strain in a seed medium and carrying out shake cultivation so as to obtain a seed liquid; 2) inoculating the seed liquid obtained in the step 1) into a sugar-producing medium, continuing shake fermentation cultivation so as to obtain fermentation broth, carrying out centrifugation and then collecting a supernatant; and 3) subjecting the supernatant collected in the step 2) to filtering, wherein the obtained filtrate is the mixed liquor of glucose and fructose. The mixed liquor of glucose and fructose can be used in preparation of food additives and health product additives; the food additives and health product additives may be sweeteners which are especially added into beverages.

Description

technical field [0001] The present invention relates to sugarcane, in particular to a method for preparing a mixed solution of glucose and fructose from bagasse. Background technique [0002] Sugarcane is a plant rich in sugar and fiber. Bagasse is the main by-product of sugar cane production and is a sustainable biomass resource. Bagasse generally contains 35.4% cellulose, 20.6% hemicellulose, 18.6% lignin, 8.3% crude ash, 3.8% crude protein, 2.8% soluble sugar, 1.5% starch, and 3.3% uronic acid. Sugarcane has a large amount of bagasse remaining after sugar production. According to the current annual output of 11 million tons of cane sugar in my country, the country's sugar production enterprises can have 6 to 6.5 million tons of bagasse surplus each year. Rational use of bagasse, reduction of production waste, and the realization of the recycling and development of bagasse resources have attracted increasing attention. [0003] In recent years, the main uses of bagasse ...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P19/14C12P19/02C12R1/07
Inventor 曾润颖温键易志伟产竹华陈兴麟
Owner THIRD INST OF OCEANOGRAPHY STATE OCEANIC ADMINISTATION
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