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Method for producing astaxanthin through lactobacillus fermentum

A technology of astaxanthin and lactobacillus, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., can solve the problems of strain resource limitation, achieve high antioxidant properties, mild culture conditions, and good stability Effect

Active Publication Date: 2015-03-04
广州元大生物科技发展有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The technical problem to be solved in the present invention is to provide a method for producing astaxanthin by fermenting Lactobacillus in view of the defect that the production of astaxanthin by fermentation strains in the prior art is limited by strain resources

Method used

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  • Method for producing astaxanthin through lactobacillus fermentum
  • Method for producing astaxanthin through lactobacillus fermentum

Examples

Experimental program
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Effect test

Embodiment 1

[0029] S1. Fermentation culture of Lactobacillus plantarum ATCC8014: Inoculate one ring of bacterial lawn of Lactobacillus plantarum ATCC8014 into a 250mL Erlenmeyer flask containing 12.5mL of seed medium, and culture it on a shaking table at 30°C and 180r / min for 24h. Seed liquid, 5 mL of this seed liquid was inoculated in a 500 mL Erlenmeyer flask containing 45 mL of seed liquid, and cultured on a shaker at 30°C and 180 r / min for 18 hours to obtain a secondary seed liquid, and then inoculated with an inoculation amount of 6 % (v / v) Cultivate in a fermenter (10L tank contains 7L astaxanthin-producing fermentation medium) at 30°C, use a constant pH, maintain a relative dissolved oxygen content of 40-60%, and obtain a dry cell weight of 26 g / L astaxanthin Yield 598mg / L.

[0030] 60g / L sucrose, 10g / L molasses, 5g / L ammonium sulfate and 350 μL / L α-pinene contained in the astaxanthin-producing fermentation medium, 0.9 μg / L vitamin B 12 . , the pH of the medium was 7.0.

[0031]...

Embodiment 2

[0036] S1. Fermentation culture of Lactobacillus plantarum ATCC8014: Inoculate one ring of bacterial lawn of Lactobacillus plantarum ATCC8014 into a 250mL Erlenmeyer flask containing 12.5mL of seed medium, and culture it on a shaking table at 30°C and 180r / min for 24h. Seed solution, 5mL of this seed solution was inoculated into a 500mL Erlenmeyer flask containing 45mL of seed solution, cultured on a shaker at 30°C and 180r / min for 18h to obtain a secondary seed solution, and then inoculated with an inoculation amount of 8% (v / v) Cultivate in a fermenter (10L tank contains 7L astaxanthin-producing fermentation medium) at 28°C, use a constant pH, maintain a relative dissolved oxygen content of 40-60%, and obtain a dry cell weight of 28 g / L astaxanthin Yield 456mg / L.

[0037] The astaxanthin-producing fermentation medium contains 60g / L sucrose, 10g / L molasses, 5g / L ammonium sulfate and 150 μL / L α-pinene, 0.2 μg / L vitamin B 12 , the pH of the medium was 7.0.

[0038] S2. Pr...

Embodiment 3

[0041] S1. Fermentation culture of Lactobacillus plantarum ATCC8014: Inoculate one ring of bacterial lawn of Lactobacillus plantarum ATCC8014 into a 250mL Erlenmeyer flask containing 12.5mL of seed medium, and culture it on a shaking table at 30°C and 180r / min for 24h. Seed liquid, 5 mL of this seed liquid was inoculated into a 500 mL Erlenmeyer flask containing 45 mL of seed liquid, cultured on a shaker at 30°C and 180 r / min for 18 hours to obtain a secondary seed liquid, and then inoculated with 10 % (v / v) inoculum Cultivate in a fermenter (10L tank contains 7L astaxanthin-producing fermentation medium) at 26°C, use a constant pH, maintain a relative dissolved oxygen content of 40-60%, and obtain a dry cell weight of 19 g / L astaxanthin Yield 513mg / L.

[0042] 120 sucrose, 4.5 g / L urea and 500 μL / L α-pinene contained in the astaxanthin-producing fermentation medium, 0.8 μg / L vitamin B 12 , the medium pH was 6.

[0043] S2. Preparation and purification of crude pigment: ...

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Abstract

The invention relates to the technical field of compound preparation, and specifically discloses a method for producing astaxanthin by fermenting Lactobacillus. The method is that by adding α-pinene and vitamin B12 to the fermentation medium, the lactobacillus that does not produce astaxanthin can be effectively induced to produce astaxanthin in large quantities, and the astaxanthin yield and biomass of the astaxanthin produced by it can be as high as about 600mg / L and 26g / L. The method of the present invention is a new way to produce natural astaxanthin by biosynthesis, and its excellent production performance makes it expected to be a kind of astaxanthin production that can replace Phaffia rhodozyma and Haematococcus pluvialis to produce astaxanthin method.

Description

technical field [0001] The invention relates to the technical field of compound preparation, in particular to a method for producing astaxanthin by fermenting Lactobacillus. Background technique [0002] Astaxanthin (3,3'-dihydroxy-4,4'-diketo-ββ'-carotene) is a non-vitamin A source of ketocarotenoids. With bright red color and strong pigmentation ability, it was originally used as a bait additive in aquaculture as a pigment in some developed countries in Europe and America. Astaxanthin has strong antioxidant properties. Animal experiments have shown that it has various physiological effects such as inhibiting tumorigenesis and enhancing immune function. As a functional pigment, it has a wide range of applications in aquaculture, medicine, food, cosmetics, etc. prospect. [0003] Most of the astaxanthin currently on the market is chemically synthesized, and there is also a small amount of natural astaxanthin. The main purpose of chemically synthesized astaxanthin is as a ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P23/00C12R1/25C12R1/245C12R1/24C12R1/225
Inventor 廖美德蹇华丽宋光均
Owner 广州元大生物科技发展有限公司
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