Acid proteinase culture medium

A technology of acid protease and medium, which is applied in the field of acid protease medium to achieve the effects of increasing the amount, improving permeability, and accelerating growth and metabolism

Inactive Publication Date: 2015-09-02
黄红林
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Acid protease is a kind of hydrolase that breaks peptide chains. Its pH is around 2 to 4. It mainly comes from the organs of animals. The most thorough research is pepsin, but by improving the formula of the medium, the enzyme production of the strain can be increased. is research that has not yet reported

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] An acidic protease medium, comprising the following components and the ratio of each component is: corn flour: 8 parts, fish meal: 12 parts, bean cake powder: 30 parts, dipotassium hydrogen phosphate: 3 parts, calcium chloride: 4 parts , ammonium chloride: 4 parts, sodium chloride: 2 parts, calcium oxide: 5 parts, combined enzyme: 10 parts, medium hydrolyzate: 6 parts, carbon source supplement: 8 parts, nitrogen source supplement: 4 parts , energy supplement: 4 parts, surfactant: 5 parts, oxygen carrier: 5 parts, the combined enzyme is a mixed solution of cellulase, helicase, and lysozyme, and the ratio is 6.5:2.4:1.3 in sequence. The carbon source supplement uses dextrin, the nitrogen source supplement uses peptone, the energy supplement uses sodium dihydrogen phosphate, the surfactant uses benzylphenoxyammonium chloride, and the oxygen carrier uses n-dodecane .

Embodiment 2

[0020] An acidic protease medium, comprising the following components and the ratio of each component is: corn flour: 12 parts, fish meal: 14 parts, bean cake powder: 35 parts, dipotassium hydrogen phosphate: 7 parts, calcium chloride: 6 parts , ammonium chloride: 30 parts, sodium chloride: 4 parts, calcium oxide: 7 parts, combined enzyme: 30 parts, medium hydrolyzate: 15 parts, carbon source supplement: 14 parts, nitrogen source supplement: 6 parts , energy supplement: 15 parts, surfactant: 15 parts, oxygen carrier: 10 parts, the combined enzyme is a mixed solution of cellulase, helicase, and lysozyme, and the ratio is 6.5:2.4:1.3 in sequence. The carbon source supplement uses potato starch, the nitrogen source supplement uses bean cake hydrolyzate, the energy supplement uses potassium dihydrogen phosphate, the surfactant uses nonylphenol polyoxyethylene ether, and the oxygen carrier uses whole Fluorocarbons.

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PUM

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Abstract

The invention discloses an acid proteinase culture medium. The acid proteinase culture medium comprises, by portion, the components of 7-12 parts of corn flour, 11-14 parts of fish meal, 25 to 35 parts of soybean cake powders, 2-7 parts of dipotassium phosphate, 4-6 parts of calcium chloride, 15 to 30 parts of ammonium chloride, 1-4 parts of sodium chloride, 20-50 parts of calcium oxide, 10-30 parts of combined enzyme, 5-15 parts of culture medium hydrolysate, 6-14 parts of carbon source replenishers, 4-6 parts of nitrogen source replenishers, 3-15 parts of energy replenishers, 5-15 parts of surface active agent and 5-10 parts of oxygen carrier. The acid proteinase culture medium has the advantages that the yield is high, the enzyme activity is high, the fermentation level is improved, the enzyme activity is improved, and the protease is composited efficiently.

Description

【Technical field】 [0001] The invention relates to the technical field of acid protease medium. 【Background technique】 [0002] Acid protease is refined by fermenting and refining the excellent Aspergillus niger strain of Longkete. It can effectively hydrolyze protein under low pH conditions and is widely used in alcohol, liquor, beer, brewing, food processing, feed addition, and leather processing. and other industries. Acid protease is a kind of hydrolase that breaks peptide chains. Its pH is around 2 to 4. It mainly comes from the organs of animals. The most thorough research is pepsin, but by improving the formula of the medium, the enzyme production of the strain can be increased. is research that has not yet been reported. 【Content of invention】 [0003] The purpose of the present invention is to solve the problems in the prior art, and propose an acidic protease culture medium, which has the advantages of high yield, high enzyme activity, improved fermentation leve...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/62
CPCC12N9/62
Inventor 杨海霞黄红林
Owner 黄红林
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