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Application of compound 2,4-dyhydroxyl-5-methyl-acetophenone separated and prepared with fermentation broth of Polyporus picipes

A technology of porous bacteria and fermentation broth, which is applied in the fields of chemicals, applications, and fungicides for biological control. Simple and easy, short fermentation time, mild culture conditions

Inactive Publication Date: 2013-06-26
NORTHWEST A & F UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Related literature [A. A. Morais, R.B. Fo, S.V. Fraiz Jr. Synthesis of three natural 1,3-diarylpropanes: Two revised structures, Phytochemistry, 1989, 28 (1) : 239-242] and [Cai Mengshen, Wang Lanming. Carbon Glycosides Research—XXVI. Synthesis of Isomidadin Analogues, Acta Chem. Sinica, 1990, 48, 1191-1198] only reported 2,4-dihydroxy-5-methyl -Acetophenone is used as a synthetic intermediate; but there is no relevant report on its activity as a natural product and anti-phytopathogenic fungi
The applicant once reported the preliminary study on the antibacterial activity of the fermented metabolites of P. brown polypore for the first time (Dong Yanhong, Zhang Anling, Ma Huini, Li Xiaoming, Gao Jinming, Journal of Northwest Forestry University, 2007, 22 (2): 105-108), however, Antimicrobial active compounds in fermentation broth are unknown

Method used

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  • Application of compound 2,4-dyhydroxyl-5-methyl-acetophenone separated and prepared with fermentation broth of Polyporus picipes
  • Application of compound 2,4-dyhydroxyl-5-methyl-acetophenone separated and prepared with fermentation broth of Polyporus picipes
  • Application of compound 2,4-dyhydroxyl-5-methyl-acetophenone separated and prepared with fermentation broth of Polyporus picipes

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Experimental program
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preparation Embodiment 1

[0033] This embodiment adopts a liquid culture method in a plate rotating flask, and the specific steps are as follows:

[0034] 1) Fermentation of strains and concentration of fermentation broth

[0035] Take 8~10 mm from the activated solid plate strain (28℃, culture for 5~6d) 2 2~3 pieces of large and small bacteria cakes are connected to a liquid PDA medium made of peeled potatoes 200g / L, glucose 20g / L, distilled water 1000mL, pH 7.2 or so, sterilized at 121°C for 30 minutes (liquid volume is 50mL / In a 150mL Erlenmeyer flask), incubate for 3 to 4 days on a rotating shaker (rotation speed: 150r / min) at 28°C to form bacteria balls and transfer them to the fermentation flask with a 10% inoculum amount (liquid volume is 150mL / 500mL) Erlenmeyer flask), fermented for 13 days to obtain 50L of fermentation broth. Concentrate 50L of fermentation broth at 55°C under reduced pressure and halve;

[0036] 2) Preparation of crude extract

[0037] The fermented product concentrate was extract...

preparation Embodiment 2

[0045] This embodiment adopts the method of liquid culture in a plate rotating flask, and the specific steps are as follows:

[0046] 1) Fermentation of strains and concentration of fermentation broth

[0047] Take 6~8mm from the activated solid plate strain (25℃, culture for 6~7d) 2 Three to four large and small bacterial cakes are connected to a liquid PDA medium made of peeled potatoes 180g / L, glucose 18g / L, distilled water 1000mL, pH 7.2 or so, sterilized at 121°C for 30 minutes (liquid volume is 50mL / In a 150mL Erlenmeyer flask), incubate at 25°C on a rotating shaker (rotational speed: 130r / min) for 4 to 5 days to form bacterial pellets and transfer to a fermentation flask with a 10% inoculum volume (liquid volume is 130mL / 500mL Erlenmeyer flask), fermented for 14 days to obtain a total of 10L of fermentation broth. Concentrate 10L of fermentation broth at 55°C under reduced pressure and halve;

[0048] 2) Preparation of crude extract

[0049] The fermented product concentrate...

preparation Embodiment 3

[0053] This embodiment adopts the method of liquid culture in a plate rotating flask, and the specific steps are as follows:

[0054] 1) Fermentation of strains and concentration of fermentation broth

[0055] Take 6~8mm from the activated solid plate strain (30℃, cultivate for 4~5d) 2 2~3 pieces of large and small bacteria cakes are inserted into the liquid PDA culture medium made of peeled potato 250g / L, glucose 25g / L, distilled water 1000mL, pH 7.2, and sterilized at 121°C for 30 minutes (filling volume is 50mL / 150mL Erlenmeyer flask), incubate at 34°C on a rotary shaker (170r / min) for 3 to 4 days to form bacteria pellets and transfer to the fermentation flask at a 10% inoculum (liquid volume 170mL / 500mL Erlenmeyer flask ), fermented for 11 days, a total of 15L of fermentation broth was obtained. Concentrate 15L of fermentation broth at 55°C under reduced pressure and halve;

[0056] 2) Preparation of crude extract

[0057] The fermented product concentrate was extracted with et...

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Abstract

The invention relates to a method for separating and preparing compound 2,4-dyhydroxyl-5-methyl-acetophenone (II) with the fermentation broth of Polyporus picipes. The method adopting a plate-to-bottle liquid culture method includes the following steps: a peeled potato, glucose and distilled water, the pH of which is about 7.2, are first sterilized and prepared into culture medium; the culture medium is then fermented into fermentation broth, the fermentation broth is extracted with ethyl acetate to obtain extract, and the extract is separated by column chromatography, and is eluted at a gradient by a chloroform-methanol system; and after separation by multiple times of normal-phase silica gel column chromatography, a reversed-phase C18 column, SephadexLH-20 gel column chromatography and recrystallization, the pure compound 2,4-dyhydroxyl-5-methyl-acetophenone (II) is obtained. The invention can develop the compound 2,4-dyhydroxyl-5-methyl-acetophenone (II) into the technical product of pathogenic fungus-resistant antibiotic, solves the problem of material resources for the 2,4-dyhydroxyl-5-methyl-acetophenone, and has the advantages of mild culture conditions, simple and easy culture medium preparation, short fermentation time and the like.

Description

[0001] This application is a Chinese patent application (application number: 200910020963.7, application date: January 19, 2009), titled "Preparation of compound 2,4-dihydroxy-5-methyl-phenylethyl ether by separation of fermentation broth of P. A divisional application for the method of ketones. technical field [0002] The present invention relates to a preparation method of a compound, in particular to a method for separating and preparing compound 2,4-dihydroxy-5-methyl-acetophenone (II) by using the fermented liquid of Poreus pheloides. The compound 2,4-dihydroxy-5-methyl-acetophenone (II) prepared by the present invention is a new natural product, which can inhibit the growth of various plant pathogenic fungi, and is effective against cotton fusarium wilt, Curvularia maize, Apple anthracnose bacteria have a good inhibitory effect. Background technique [0003] Higher fungi include some species of Ascomycetes (Ascomycetes) and Basidiomycetes (Basidiomycetes) fungi. Due...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N35/04A01P3/00
Inventor 高锦明李勇解思达张鞍灵石新卫张炜玲
Owner NORTHWEST A & F UNIV
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