Synthesis of beta-dihydrolignaloefuransesquiter polyalcoholate compounds

A synthetic method, the technology of picrocetin, which is applied in the field of transgenic culture and synthesis of B-dihydroagarfuran sesquiterpene polyol esters, can solve the problems of difficult chemical synthesis, limited development and application, complex structure, etc., and reduce production The equipment and extraction method are simple and easy, and the effect is good.

Inactive Publication Date: 2006-02-15
赵天增 +4
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Due to its complex structure, chemical synthesis is very difficult and uneconomical, which seriously limits its further development and application.

Method used

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  • Synthesis of beta-dihydrolignaloefuransesquiter polyalcoholate compounds
  • Synthesis of beta-dihydrolignaloefuransesquiter polyalcoholate compounds

Examples

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Embodiment 1

[0013] Example 1. A new method for the synthesis of β-dihydroagarfuran sesquiterpene polyol esters, including the steps of inoculating C58 Agrobacterium, tissue culture, and extraction of the target product,

[0014] Inoculation step: first activate the C58 Agrobacterium, and inoculate the lyophilized and preserved C58 Agrobacterium on the slant solid medium A'. The composition of solid medium A' is: every 100ml solid medium contains 0.5g peptone, 0.5g beef extract, 0.5g sucrose, 24mg magnesium sulfate, 2g agar. Sterilize at 121°C for 30 minutes. The culture conditions were 25°C, one transfer every 48 hrs, and three transfers. Then inoculate on liquid medium B'. Each 100ml of liquid medium contains 1g of peptone, 0.5g of yeast extract, and 1g of sucrose, adjust the pH to 7.2 with 1N NaOH aqueous solution, and sterilize at 0.1-0.15MPa and 121°C for 30min. Culture Conditions: Cultivate on a shaker (120-160rpm) at 25°C for 36 hours, use sterile liquid culture solution as a bla...

Embodiment 2

[0017]Embodiment 2, in this embodiment, the formula of solid medium A is: magnesium sulfate 444mg / l, potassium dihydrogen phosphate 204mg / l, potassium nitrate 2800mg / l, ammonium nitrate 1980mg / l, calcium chloride 528mg / l, boric acid 7.4mg / l, manganese sulfate 18.8mg / l; zinc sulfate 10.3mg / l, sodium molybdate 0.30mg / l, copper sulfate 0.03mg / l, cobalt chloride 0.03mg / l, potassium iodide 0.99mg / l, ferrous sulfate 33.2mg / l, EDTA sodium salt 44.5mg / l, thiamine 0.6mgl / l, pyridoxine 0.6mg / l, niacin 0.06mg / l, inositol 120mg / l. Sucrose 30000mg / l, agar 12000mg / l. Others are with embodiment 1.

Embodiment 3

[0018] Embodiment 3. In this embodiment, the activation of C58 Agrobacterium and the disinfection of the leaves of Phyllostachys chinensis are the same as in Embodiment 1. The formula and preparation method of solid medium A are the same as in Example 1. The formula of liquid medium B is: magnesium sulfate 296mg / l, potassium dihydrogen phosphate 136mg / l, potassium nitrate 1520mg / l, ammonium nitrate 1320mg / l, calcium chloride 328mg / l, boric acid 5.0mg / l, manganese sulfate 12.4mg / l; zinc sulfate 6.9mg / l, sodium molybdate 0.20mg / l, copper sulfate 0.02mg / , cobalt chloride 0.02mg / , potassium iodide 0.66mg / l, ferrous sulfate 22.3mg / l, EDTA sodium salt 29.4mg / l, thiamine 0.4mgl / l, pyridoxine 0.4mg / l, niacin 0.04mg / l, inositol 80mg / l. Sucrose 30000mg / l. The preparation method is: take the above-mentioned ingredients except sucrose, dissolve them in water, mix and dilute to the required concentration with water, add sucrose to dissolve, adjust the pH to 6.0, and sterilize at 121°C ...

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Abstract

A process for synthesizing the beta-dihydrogarofuran sesquiterpene polyol ester compounds includes such steps as inoculating Agrobacterium C58 to bitterbark vine, taking crown atissue, culturing and extracting target product. Its advantages are less investment, low cost and short period.

Description

technical field [0001] The invention relates to a method for transgenic culture and synthesis of B-dihydroagarfuran sesquiterpene polyol esters. Background technique [0002] B-dihydroagarwood furan sesquiterpene polyol esters are a class of substances with insecticidal activity and insect feed resistance. This type of compound has the characteristics of good insecticidal effect, low lethality to natural enemies, and low toxicity to humans and animals. As a high-efficiency, low-toxicity and pollution-free insecticide. But such compounds only exist in the natural plant Phyllostachys chinensis, and the content is very low. Due to its complex structure, chemical synthesis is very difficult and uneconomical, which seriously limits its further development and application. B-dihydroagarwood furan sesquiterpene polyol esters [0003] Agrobacterium is a plant pathogenic bacteria, mainly including Agrobacterium tumefaciens and Agrobacterium rhizogenes, which can infiltrate the inj...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/62C12P17/02C12R1/01
Inventor 赵天增傅经国吴鸣健范辑玉
Owner 赵天增
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