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Method for synthesizing secoiridoid glycosides compound

A technology for split ring iridoids and compounds, which is applied in the field of biotransformation and synthesis of organic compounds, can solve the problems of restricted development and utilization, complex chemical structure, difficulty in total synthesis, etc. The effect of incubation time

Inactive Publication Date: 2008-01-16
河南宝隆生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the content of such compounds in natural plants is very low, and their chemical structures are complex. It is very difficult and uneconomical to carry out total synthesis by chemical methods, which seriously limits their further development and utilization.

Method used

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  • Method for synthesizing secoiridoid glycosides compound
  • Method for synthesizing secoiridoid glycosides compound

Examples

Experimental program
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Effect test

Embodiment 1

[0016] Example 1, a method for synthesizing secoiridoid glycosides, taking 370 mg of magnesium sulfate, 170 mg of potassium dihydrogen phosphate, 1900 mg of potassium nitrate, 1650 mg of ammonium nitrate, 440 mg of calcium chloride, 1.0 mg of thiamine, inositol 100mg, boric acid 6.2mg, manganese sulfate 15.6mg, zinc sulfate 8.6mg, sodium molybdate 0.025mg, copper sulfate 0.025mg, cobalt chloride 0.025mg, potassium iodide 0.085mg, ferrous sulfate 27.8mg, EDTA sodium salt 37.3mg, 2 , 4-D 1.0mg, 6-furfurylaminoadenine 2.0mg, naphthaleneacetic acid 3.0mg, sucrose 30000mg, agar 12000mg, weighed the medium components except sucrose and agar one by one, dissolved and mixed with deionized water, and then weighed Take the formulated amount of sucrose and agar, add to the above solution to dissolve and add deionized water to dilute to 1000ml, adjust the pH to 5.8, and sterilize at 121°C for 25 minutes to obtain a solid medium.

[0017] Sterilize the leaves, young shoots or tender stems ...

Embodiment 2

[0018] Example 2, in this example, the sterilized privet leaves were placed on the sterilized solid medium, induced and cultivated in a 28°C incubator for 49 days, the product was taken out, and placed in a Soxhlet extractor each time Add 15 times the weight of absolute ethanol to extract twice, and then distill off the ethanol to obtain the target molecule. Others are with embodiment 1.

Embodiment 3

[0019] Example 3, a method for synthesizing secoiridoid glycosides. In this example, the solid medium and the preparation method are the same as in Example 1. The formula of the liquid medium is magnesium sulfate 370mg, potassium dihydrogen phosphate 170mg, potassium nitrate 1900mg, ammonium nitrate 1650mg, calcium chloride 440mg, thiamine 1.0mg, inositol 100mg, boric acid 6.2mg, manganese sulfate 15.6mg, zinc sulfate 8.6mg , sodium molybdate 0.025mg, copper sulfate 0.025mg, cobalt chloride 0.025mg, potassium iodide 0.085mg, ferrous sulfate 27.8mg, EDTA sodium salt 37.3mg, 2,4-D 1.0mg, 6-furfurylaminoadenine 2.0mg , naphthaleneacetic acid 3.0mg, sucrose 30000mg, weigh the above medium components one by one, dissolve and mix with deionized water, and add deionized water to dilute to 1000ml, adjust the pH to 5.6, and sterilize at 121°C for 1 minute to obtain a liquid medium .

[0020] Sterilize the leaves of Lilac lobularis and place them on solid medium, culture them at 28°C f...

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Abstract

The present invention relates to a method for synthesizing secoiridoid glycoside compound, belonging to the field of biolgoical conversion synthesis technology of organic compound. Said method includes four steps of preparation of culture medium, induction of explant, tissue (cell) culture and extraction of target molecule. Said invention only can utilize solid culture medium to make culture and extraction, also can utilize solid culture medium to make culture firstly, then utilize liquid culture medium to make culture and extraction. Said method and process are simple, and high in yield.

Description

technical field [0001] The invention belongs to the technical field of biotransformation and synthesis of organic compounds, and in particular relates to a method for synthesizing secoiridoid glycosides by using a tissue (cell) culture method. Background technique [0002] The secoiridoid glycosides are a class of substances with antibacterial, anti-inflammatory, anti-viral, anti-oxidant, anti-cancer, hypoglycemic and other effects, and have very low toxicity, and can be used in medical and health care functions. However, the content of such compounds in natural plants is very low, and their chemical structures are complex. It is very difficult and uneconomical to fully synthesize them by chemical methods, which seriously limits their further development and utilization. Contents of the invention [0003] The object of the present invention is to provide a biotransformation synthesis method for synthesizing secoiridoid glycosides, that is, a tissue (cell) culture method su...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H17/06C12N5/04
Inventor 赵天增吴鸣建董建军傅经国张海艳
Owner 河南宝隆生物技术有限公司
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