Process for continuously extracting cordyceps militaris essential oil, cordycepin, cordycepic acid and cordyceps militaris polysaccharide from cordyceps militaris

A technology of Cordyceps polysaccharides and Cordyceps militaris, which is applied in the direction of sugar derivatives, fat oil/fat production, non-central analgesics, etc. It can solve the problems of low extraction rate, high amount of organic solvent usage, complicated process, etc., and achieve simplified separation and purification Steps to improve production efficiency and reduce production costs

Active Publication Date: 2009-11-18
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method extracts cordycepin and cordyceps polysaccharide, the extraction rate is low, the process is complicated, only cordycepin and cordyceps polysaccharide can be extracted; but the volatile oil of cordycepin, cordycepin, cordycepic acid and cordyceps polysaccharide cannot be extracted continuously, and the use of its organic solvent higher volume

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] (1) Take the liquid-fermented Cordyceps militaris mycelium, dry in a vacuum box at 60°C for 12 hours, pulverize in a plant pulverizer, pass through a 40-mesh sieve to obtain Cordyceps militaris mycelium powder, and accurately weigh 1kg of Cordyceps militaris mycelium Body powder, added to the extraction kettle with a volume of 3 liters of supercritical CO 2 In the extraction kettle of the fluid extraction instrument, set the extraction pressure to about 20Mpa, the extraction temperature to 37°C, and the CO 2 The flow rate is 80L / h, and the extraction time is 2h. Obtain bright yellow and transparent volatile oil of Cordyceps militaris from the separation kettle.

[0013] (2) Keep the Cordyceps militaris powder in the extraction kettle unchanged, change the extraction conditions to be: extraction pressure about 25Mpa, extraction temperature 40°C, entrainer is 2 liters of ethyl acetate, CO 2 The flow rate is 50L / h, and the extraction time is 2h. Obtain the ethyl acetate...

Embodiment 2

[0016] (1) Take the fruiting bodies of Cordyceps militaris cultivated by artificial solids, dry them in a vacuum box at 60°C for 12 hours, pulverize them in a plant pulverizer, and pass through a 40-mesh sieve to obtain fruiting bodies of Cordyceps militaris, and accurately weigh 1kg of fruiting bodies of Cordyceps militaris Bacteria powder, added to 3L of supercritical CO 2 In the extraction kettle of the fluid extraction instrument, set the extraction pressure to about 20Mpa, the extraction temperature to 40°C, and the CO 2 The flow rate is 120L / h, and the extraction time is 2h. Obtain bright yellow and transparent volatile oil of Cordyceps militaris from the separation kettle.

[0017] (2) Keep the Cordyceps militaris powder in the extraction kettle unchanged, and change the extraction conditions to be: about 25Mpa extraction pressure, 40°C extraction temperature, 2 liters of ethyl acetate as entrainer, 50L / h CO2 flow rate, and 2h extraction time. Obtain the ethyl acetate...

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PUM

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Abstract

The invention discloses a process for continuously extracting cordyceps militaris essential oil, cordycepin, cordycepic acid and cordyceps militaris polysaccharide from cordyceps militaris. Cordyceps militaris mycelium powder screened by a 40 to 60 mesh sieve is added into a supercritical CO2 fluid extraction apparatus; the step of extraction is carried out for 1 to 3 hours under the conditions that the pressure is 15 to 25 MPa, the temperature is 35 to 40 DEG C and the flow rate is 50 to 150 L / min CO2, and the cordyceps militaris essential oil is separated out from a separation kettle; ester or ketone organic entrainer of which the mass is 1 to 3 times that of the cordyceps militaris mycelium powder is used for continuous extraction for 1 to 3 hours so as to separate out the cordycepin; and distilled water of which the mass is 1 to 3 times that of the cordyceps militaris mycelium powder is used as an entrainer for extraction for 1 to 3 hours, and the first grade separate mode is adopted for separating out an aqueous solution containing the cordycepic acid and the cordyceps militaris polysaccharide. The process reduces the usage of organic solvent, increases the production efficiency and reduces the production cost. The extraction yield of the obtained cordyceps militaris essential oil is nearly 6.5 percent, the extraction yield of the cordycepin is nearly 0.049 percent, the purity of the cordycepin is nearly 45 percent, and the extraction yield of the cordycepic acid is nearly 2 percent.

Description

technical field [0001] The present invention relates to a kind of supercritical CO 2 Process for fluid continuous extraction of Cordyceps volatile oil, cordycepin, cordycepic acid and cordyceps polysaccharide from Cordyceps militaris. Background technique [0002] Cordyceps sinensis is a kind of rare and precious medicinal material with high medicinal value. It contains many active ingredients, such as cordycepic acid, cordyceps polysaccharide, various nucleosides (cordycepin, uridine, adenosine), volatile oil, flavonoids, sterol compounds, superoxide dismutase, etc. Among them, the contents of Cordyceps polysaccharide, cordycepin, and cordycepic acid are the main indicators for evaluating the quality of Cordyceps. Among them, cordycepin is a nucleoside antibiotic, which has various biological activities such as anti-tumor, anti-virus, anti-bacterial and anti-inflammatory, improving immunity, and anti-leukemia. Cordyceps polysaccharide can regulate human immune function, ...

Claims

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Application Information

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IPC IPC(8): C11B1/10C07H19/16C07C31/26C08B37/00A61P35/00A61P31/12A61P31/04A61P29/00A61P37/04A61P35/02A61P3/10A61P9/10A61P7/02A61P7/04A61K36/066
CPCY02P20/54
Inventor 刘东波谢红旗夏志兰熊兴耀
Owner HUNAN AGRICULTURAL UNIV
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