Method utilizing column chromatography to prepare high-purity L-alpha glyceryl phosphoryl ethanolamine

A technology of glycerol phosphatidylethanolamine and column chromatography, which is applied in the field of deep processing of phospholipids, can solve problems such as L-α-GPE research reports that have not yet appeared, and achieve the effects of high industrial development prospects, low cost, and simple process

Active Publication Date: 2012-07-04
无锡食赫兹未来食品科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no domestic research report on L-α-GPE

Method used

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  • Method utilizing column chromatography to prepare high-purity L-alpha glyceryl phosphoryl ethanolamine
  • Method utilizing column chromatography to prepare high-purity L-alpha glyceryl phosphoryl ethanolamine
  • Method utilizing column chromatography to prepare high-purity L-alpha glyceryl phosphoryl ethanolamine

Examples

Experimental program
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Effect test

Embodiment 1

[0018] Weigh 250g of soybean powder phospholipids into a beaker, add 1L of anhydrous methanol, stir quickly to make the powder phospholipids in the system evenly diffuse, then add 12g of sodium methoxide, stir at 35°C for 6h, and filter with suction, adjust the pH of the obtained clear liquid to 9 about. Measure 200ml of methanol-soaked D001 resin, pack it into a column, wash the column with 400ml of methanol, and load the alcoholysis solution of soybean powder phospholipids at a flow rate of 1.5mL / min. After loading, first elute with 1L methanol, and then use The column was desorbed with ultrapure water at pH=6.78. Load the desorbed liquid again into a chromatographic column (washed with 400mL water) equipped with 100mL201×7 resin, and the effluent under the column is GPC aqueous solution. After loading the sample, wash it with water until the GPC flows out completely. The purity of GPC is 97.11 %, and then desorbed with 1% acetic acid, the resulting desorbed solution was GP...

Embodiment 2

[0020] Weigh 250g of soybean powder phospholipids into a beaker, add 1.25L of anhydrous methanol, stir quickly to make the powder phospholipids in the system evenly diffuse, then add 10g of sodium methoxide, stir at 35°C for 19h, and filter with suction, the pH of the obtained clear liquid reaches 8 about. Measure 100ml of D001 resin soaked in methanol, pack it into a column, wash the column with 200ml of methanol, and load the above alcoholysis solution of soybean powder phospholipid at a flow rate of 2.0mL / min. After loading, first elute with 1L methanol, and then use The column was desorbed with ultrapure water at pH=5.01. Load the desorbed solution again into a chromatography column (washed with 400mL water) equipped with 200mL 201×7 resin. The effluent under the column is GPC aqueous solution. After loading the sample, wash it with water until the GPC flows out completely. The purity of GPC is 95.72 %, and then desorbed with 1% acetic acid, the obtained desorbed solution...

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Abstract

The invention discloses a method utilizing column chromatography to prepare high-purity L-alpha glyceryl phosphoryl ethanolamine (L-alpha-GPE) and belongs to the technical field of deep processing of phospholipid. The method comprises adopting sodium methoxide to catalyze soybean powder phospholipid to react in an absolute methanol system so as to prepare mixed liquid containing L-alpha glyceryl phosphoryl ethanolamine, after adjusting the pH, enabling the mixed liquid to pass through a strong cation exchange resin (absolute alcohol phase equilibrium) chromatographic column and a strong anion exchange resin (water balance) chromatographic column which are both capable of being used in non-aqueous media, adopting specific desorption solution to carry out desorption, finally performing reduced pressure distillation to the desorption solution, and obtaining light yellow transparent sticky liquid which is an L-alpha glyceryl phosphoryl ethanolamine product. The product obtained by the column chromatography is high in purity and low in cost, L-alpha-Glycerophosphorylcholine with high purity can be separated simultaneously, and the product is simple in process and suitable for industrial production. The method expands application range of deep processing products of phospholipid.

Description

technical field [0001] The invention relates to a method for preparing high-purity glycerol phosphatidylethanolamine (L-alpha glycerylphosphorylethanolamine, L-α-GPE), which belongs to the technical field of phospholipid deep processing. Background technique [0002] Glycerol phosphatidylethanolamine (L-alpha glycerylphosphorylethanolamine, L-α-GPE), is the product of two fatty acyl groups on the phosphatidylethanolamine (PE) molecule being completely decomposed, and can be used in the pharmaceutical industry as the main active ingredient , assisted by appropriate drug carriers, for the treatment of chronic brain nerve tissue syndromes caused by brain degeneration or cerebrovascular insufficiency. The structural formula of the R group in its structural formula is CH 2 CH 2 NH 2 , Different from other alcoholysis products of soybean powder phospholipids, such as glycerol phosphatidylcholine (L-α-GPC), glycerol phosphatidylserine (L-α-GPS), etc. Due to the complex composit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07F9/10
Inventor 刘元法周丽张康逸王兴国
Owner 无锡食赫兹未来食品科技有限公司
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