The invention discloses a dithiophospholipid compound and a preparation method thereof. The preparation method comprises the following steps: weighing cystamine hydrochloride and triethylamine absolute methanol, adding di-tert-butyl dicarbonate, and reacting to obtain single protection cystamine; weighing fatty acid, 1-(3-dimethylaminopropyl)-3-ethylcarbodiimide hydrochloride and N-hydroxythiosuccinimide, dissolving in dichloromethane, adding mono-protected cystamine, mixing, dissolving reactants in dichloromethane, and adding trifluoroacetic acid to obtain a solid; dissolving the solid in dichloromethane, adding triethylamine and succinic anhydride, and reacting to obtain an intermediate product; and weighing the intermediate product according to the ratio, dissolving dicyclohexylcarbodiimide and 4-dimethylaminopyridine in dimethyl sulfoxide, activating, adding choline glycerophosphate, and reacting to obtain the target product. The structure of the dithiophospholipid compound provided by the invention contains a disulfide bond which is easily reduced by glutathione, so that phospholipid is broken in the presence of GSH, and the dithiophospholipid compound has a GSH response breaking function.