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Method for preparing glycerol phosphatidylcholine by enzyme method

A technology for preparing glycerol phosphatidylcholine and enzymatic method, applied in the field of oil processing, can solve the problems of shortening reaction time, long reaction time, increasing the difficulty of separation and purification, etc., achieves simple separation and purification steps, increases difficulty, and improves production rate Effect

Active Publication Date: 2021-02-23
SHAANXI UNIV OF SCI & TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In conclusion, the yield of glycerol phosphatidylcholine is higher when enzymatic hydrolysis is used to prepare glycerol phosphatidylcholine, but the reaction time is longer; although by changing the reaction system and adding Ca 2+ It can significantly increase the rate of hydrolysis reaction and greatly shorten the reaction time, but it increases the difficulty of subsequent separation and purification

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Accurately weigh a certain amount of lecithin and mix it with deionized water (a total of 10Kg, the concentration of lecithin in deionized water is 30mg / mL), stir at a speed of 400rpm for 20min to emulsify, the reaction mixture is transported to the packed bed through a material pump The reactor is reacted, and the filling enzyme is immobilized phospholipase A 1 (Using physical adsorption method or covalent binding method, according to phospholipase A 1 The ratio to the immobilized carrier is 20-80mg / g resin, stirred at 50-200rpm at 30°C for 1-8h), the filling amount is 0.5Kg, and the reaction temperature is 55°C. Then the reaction material enters the heat treatment device and stays at 60°C for 40 minutes. After the heat-treated reaction material is cooled to 55°C, it is pumped into the material tank again for circular reaction. Adjust the flow rate of the reaction substrate so that each cycle is mixed with immobilized phospholipase A 1 The contact reaction time is 20...

Embodiment 2

[0032] Accurately weigh a certain amount of lecithin and mix it with deionized water (a total of 10Kg, the concentration of lecithin in deionized water is 55mg / mL), after stirring at a speed of 600rpm for 15min to emulsify, the reaction mixture is transported to the packed bed through a material pump The reactor is reacted, and the filling enzyme is immobilized phospholipase A 1 (Prepare immobilized phospholipase A by the method described in the present invention 1), the filling amount is 2Kg, and the reaction temperature is 50°C. Then the reaction material enters the heat treatment device and stays at 70°C for 20 minutes. After the heat-treated reaction material is cooled to 50°C, it is pumped into the material tank again for circular reaction. Adjust the flow rate of the reaction substrate so that each cycle is mixed with immobilized phospholipase A 1 The contact reaction time is 10min. After 3 hours of cyclic reaction, samples were taken to measure the composition of the...

Embodiment 3

[0034] Accurately weigh a certain amount of lecithin and mix it with deionized water (a total of 10Kg, the concentration of lecithin in deionized water is 45mg / mL), after stirring at a speed of 400rpm for 20min to emulsify, the reaction mixture is transported to the packed bed through a material pump The reactor is reacted, and the filling enzyme is immobilized phospholipase A 1 (Prepare immobilized phospholipase A by the method described in the present invention 1 ), the filling amount is 1.5Kg, and the reaction temperature is 35°C. Then the reaction material enters the heat treatment device and stays at 65°C for 30 minutes. After the heat-treated reaction material is cooled to 35°C, it is pumped into the material tank again for circular reaction. Adjust the flow rate of the reaction substrate so that each cycle is mixed with immobilized phospholipase A 1 The contact reaction time is 15min. After 3 hours of cyclic reaction, samples were taken to measure the composition of ...

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Abstract

The invention discloses a method for preparing glycerol phosphatidylcholine by an enzyme method and belongs to the technical field of grease processing. After an enzyme is in contact with a substrate,a ratio of sn2-lysophospholipid to sn1-lysophospholipid in a hydrolysate is regulated and controlled through heat treatment, a conversion from the sn2-lysophospholipid to the sn1-lysophospholipid ispromoted, then generation of the glycerol phosphatidylcholine is promoted, and a generation rate of the glycerol phosphatidylcholine is greatly increased. In addition, no metal ion is added as a coenzyme factor in a reaction process and subsequent separation and purification steps are simple. The method is simple to operate and can remarkably increase the generation rate of the glycerol phosphatidylcholine, metal ions do not need to be added in the reaction process, the subsequent separation and purification steps are simple, and the method has good economic benefits and industrial applicationprospects.

Description

technical field [0001] The invention belongs to the technical field of oil processing, in particular to a method for enzymatically preparing glycerol phosphatidylcholine. Background technique [0002] Glycerol phosphatidylcholine (L-alpha glycerylphosphorylcholine, L-α-GPC) is the choline source for the synthesis of acetylcholine and phosphatidylcholine in the body. Previous studies have shown that glycerol phosphatidylcholine has important medicinal value in the treatment of Alzheimer's disease, cerebellar ataxia, schizophrenia and bipolar disorder, but as a medicinal raw material and related research Therefore, higher purity glycerol phosphatidylcholine is required. However, there is less glycerol phosphatidylcholine from natural sources, so it is of great significance to research and develop the preparation process of high-purity glycerol phosphatidylcholine. [0003] At present, the commonly used methods for preparing glycerol phosphatidylcholine mainly include enzymat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P13/00C07F9/09
CPCC12P13/001C12P7/6481C07F9/091
Inventor 李道明张佳浩周端王盼雪刘宁
Owner SHAANXI UNIV OF SCI & TECH
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