Preparation method of industrial egg yoke reaction dry culture medium
A technology for drying medium and basic medium, which is applied in the field of industrialized yolk reaction dry medium preparation, can solve the problems of high difficulty, cumbersome preparation, complicated operation, etc., and achieves the advantages of small error in experimental results, expansion of application fields, and simplified experimental process. Effect
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Embodiment 1
[0018] A kind of preparation method of industrialized anaerobic egg agar dry medium, its step comprises:
[0019] (1) Extraction of effective components of egg yolk
[0020] Take 20g of egg yolk, extract with 200ml of acetone three times, filter, and combine the filtrate; add 200ml of ethanol to the filter residue and extract three times, filter and combine the filtrate; distill and remove the ethanol respectively to obtain the ethanol extract; distill and remove the acetone to obtain the acetone extract;
[0021] (2) Preparation of Egg Yolk Suspension
[0022] Take 0.3g of the above-mentioned ethanol extract and 0.1g of the above-mentioned acetone extract, then add 0.3g of TritonX-10 surfactant, 0.2g of trioctylmethylammonium, CaCl 2 0.1g, mix it, add 10ml 9% sodium chloride aqueous solution and grind it, make it emulsify evenly, and make egg yolk emulsion suspension, the nutrient composition can be adjusted according to the needs of bacterial growth;
[0023] (3) Weigh ea...
Embodiment 2
[0032] A kind of preparation method of industrialization MYP agar (mannose egg yolk polymyxin agar) dry medium, its step comprises:
[0033] (1) Preparation of Egg Yolk Suspension
[0034] Take 0.35g of lecithin, 0.001g of glyceryl trilaurate, then add 0.3g of TritonX-45 surfactant, 0.3g of trioctylamine, FeSO 4 0.001g, mix it, add 10ml of 9% sodium chloride aqueous solution and grind it, make it emulsify evenly, and make egg yolk emulsion suspension, the nutrient composition can be adjusted according to the needs of bacterial growth;
[0035](2) Weigh each component of MYP agar preparation A: meat extract 1.0g, peptone 10.0g, D-mannitol 10.0g, sodium chloride 10.0g, phenol red 0.025g, agar 15.0g (add appropriate amount of agar powder, adjust Medium hardness), distilled water 900ml, mix each component in distilled water, use NaHCO 3 or NaOH to adjust the pH value to 7.0. Take 100ml of the egg yolk emulsion suspension prepared above, which is preparation B, mix it with the i...
Embodiment 3
[0045] A kind of industrialization TSC agar (trypsin-sulfite cycloserine agar), the preparation method of dry culture medium, its step comprises:
[0046] (1) Preparation of Egg Yolk Suspension
[0047] Get 0.4g of cephalin, 0.1g of hexacetacylglycerol, 0.1g of TritonX-405 surfactant, 0.5g of cholamine, and 0.5g of ZnSO 4 0.2g, mix it, add 10ml 9% sodium chloride aqueous solution to grind, make it emulsified evenly, and make egg yolk emulsion suspension, the nutritional content can be adjusted according to the needs of bacterial growth;
[0048] (2) Weigh the ingredients of TSC agar basal medium preparation A according to the dosage of the traditional preparation method: trypsin 15.0g, soybean peptone 5.0g, yeast extract 5.0g, sodium sulfite (meta) 1.0g, ferric ammonium citrate 1.0g, 20.0g agar (according to the hardness requirements of the medium, add agar in an appropriate amount), 1.0L distilled water, dissolve each component in distilled water, and use NaHCO 3 or NaOH so...
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