Preparation method of industrial egg yoke reaction dry culture medium

Abstract

A method for preparing industrialized egg yolk reaction drying medium, which comprises the following steps: extract the effective ingredients of egg yolk with absolute ethanol and acetone or use other glycerophospholipids, neutral fats, TritonX surfactants, and organic amine derivatives cationic surface Active agent or polyethylene glycol surfactant, mixed with metal cations, adding sodium chloride aqueous solution to grind, make it emulsified evenly, and prepare yolk emulsion suspension; then weigh the basal medium or preparation A with yolk reaction Mix with egg yolk suspension, adjust the pH value of the medium, and then dry, pulverize, sieve, and subpackage to prepare various egg yolk reaction dry culture medium. The method makes the industrialized production of the yolk reaction dry medium possible, the preparation is simple, the experimental process is simplified, the use is convenient, the cost is low, and the error of the experimental result is small, so the application field of the yolk reaction medium is greatly expanded.

Description

(1) Technical field [0001] The invention belongs to a method for preparing a microbiological test medium, in particular to a method for preparing an industrialized egg yolk reaction drying medium. (2) Background technology [0002] In the field of microbiology, media are divided into two categories due to their different preparation methods: drying media and non-drying media. The dry medium is widely used because of its simple preparation process, convenient use and industrial production. With the continuous development of microbial technology, many non-dryable media have been developed into dry media. However, at present, the culture medium containing egg yolk components cannot be made into a dry medium because it contains heat-unstable components, and can only be prepared temporarily when used. The preparation process of the traditional egg yolk reaction medium is divided into three steps: ① The medium is sterilized by high temperature and the stable ingredients are mixe...

AI Technical Summary

Problems solved by technology

From the above preparation process, it can be seen that the preparation is very cumbersome and difficult due to the heat-unstable components contained in the egg yolk, and improper temperature and aseptic operation may cause the experiment to fail at any time

For this reason, the British Oxoid.Ltd. company developed the "concentrated egg yolk emulsion suspension" and applied for a patent. The British patented product is actually the preparation B in the above-mentioned traditional preparation process, which does not solve the problem of the instability of the egg yolk components when exposed to heat. , is only an intermediate product, the preparation process of yolk reaction medium also needs to mix preparation A and preparation B, the operation is also complicated, and the industrial production of yolk reaction medium cannot be realized

Because the egg yolk reaction medium has the shortcomings of being unstable when exposed to heat and cannot be produced industrially, which brings great inconvenience to the inspection, such as high cost, complicated preparation, poor accuracy and reproducibility, etc. Many domestic inspectors mention the preparation of egg yolk reaction I feel a headache

At present, researchers in the United States, Japan and many other developed countries are overcoming this technical problem, but have not yet developed a dry medium for yolk reaction.