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A kind of Phalaenopsis virus-free seedling cultivation propagation method

A technology of Phalaenopsis and virus-free seedlings, which is applied in the field of cultivating and propagating Phalaenopsis virus-free seedlings, can solve the problems of plant leaf spots, affecting quality, necrotic flowers, etc., and achieve good seedling growth, improved quality, and high virus-free rate Effect

Inactive Publication Date: 2022-02-22
SHAOGUAN COLLEGE +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The spread of the virus has seriously restricted the industrialization of orchids. The virus can cause symptoms such as leaf spots, necrosis, and flower discoloration and deformity, which seriously affect its quality. At present, there is no definite drug that can effectively prevent the harm and spread of the virus. Non-toxic seedlings are the only way for industrial development, but the methods of other orchid varieties cannot be directly applied to Phalaenopsis, so it is necessary to independently develop a method for cultivating non-toxic seedlings for Phalaenopsis

Method used

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  • A kind of Phalaenopsis virus-free seedling cultivation propagation method
  • A kind of Phalaenopsis virus-free seedling cultivation propagation method

Examples

Experimental program
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Effect test

Embodiment 1

[0033] A method for cultivating and propagating Phalaenopsis virus-free seedlings, specifically comprising the following steps:

[0034] (1) Material selection

[0035] Choose Phalaenopsis plants that grow robustly and carry the virus after testing, remove the leaves and keep the stems;

[0036] (2) Material disinfection

[0037] Rinse the Phalaenopsis stem taken in the step (1) with tap water for 0.5h, soak it in 70% alcohol solution for 10s with the mass fraction, then disinfect it with 1.0% sodium hypochlorite for 10 minutes, and rinse it with sterile water for 5 times ; soak in 0.1% mercuric acid solution for 5 minutes with a mass fraction, stir continuously during the soaking process, and then rinse with sterile water for 5 times; add Tween-20 to the 0.1% mercuric solution, and add The mass fraction of Tween-20 is 0.08%;

[0038] (3) Material pretreatment

[0039] Soak the Phalaenopsis stem after disinfection in 1g / L chitosan solution for 20min;

[0040] (4) Primary ...

Embodiment 2

[0051] A method for cultivating and propagating Phalaenopsis virus-free seedlings, specifically comprising the following steps:

[0052] (1) Material selection

[0053] Choose Phalaenopsis plants that grow robustly and carry the virus after testing, remove the leaves and keep the stems;

[0054] (2) Material disinfection

[0055] The phalaenopsis stem left in the step (1) is rinsed with tap water for 2h, soaked in 70% alcohol solution for 30s with the mass fraction, then sterilized with mass fraction 1.0% sodium hypochlorite for 30 minutes, rinsed 10 times with sterile water; Soak in 0.1% mercuric acid solution for 15 minutes, stir continuously during the soaking process, and then rinse with sterile water for 10 times; add Tween-20 to the 0.1% mercuric solution, and Tween-20 in the 0.1% mercuric solution The mass fraction of -20 is 0.12%;

[0056] (3) Material pretreatment

[0057] Soak the Phalaenopsis stem after disinfection in 2g / L chitosan solution for 30min;

[0058]...

Embodiment 3

[0070] A method for cultivating and propagating Phalaenopsis virus-free seedlings, specifically comprising the following steps:

[0071] (1) Material selection

[0072] Choose Phalaenopsis plants that grow robustly and carry the virus after testing, remove the leaves and keep the stems;

[0073] (2) Material disinfection

[0074] Rinse the Phalaenopsis stem taken in step (1) with tap water for 1.5h, soak it in 70% alcohol solution for 20s with the mass fraction, then disinfect it with 1.0% sodium hypochlorite for 20 minutes, and rinse it with sterile water for 8 times ; soak in 0.1% mercuric acid solution for 10 minutes with a mass fraction, stir continuously during the soaking process, and then rinse 8 times with sterile water; add Tween-80 to the 0.1% mercuric solution, and add The mass fraction of Tween-80 is 0.10%;

[0075] (3) Material pretreatment

[0076] Soak the Phalaenopsis stem after disinfection in 1.5g / L chitosan solution for 25min;

[0077] (4) Primary cultu...

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Abstract

The invention relates to the technical field of moth orchid seedling breeding, in particular to a method for cultivating and propagating moth orchid virus-free seedlings, which specifically comprises the following steps: selecting robust and virus-carrying moth orchid plants, soaking them in chitosan solution after disinfection, and then carrying out For the primary culture, the virus-negative clustered shoots obtained in the primary culture are selected for subculture, and finally the seedlings with good growth obtained after the subculture are selected to continue the cultivation of strong seedlings, rooting culture and transplanting. The method for cultivating and propagating the virus-free seedlings of the Phalaenopsis provided by the invention can obtain the Phalaenopsis seedlings with good quality, high virus-free rate and robust growth, and realizes virus-free cultivation of the Phalaenopsis.

Description

technical field [0001] The invention relates to the technical field of moth orchid seedling breeding, in particular to a method for cultivating and propagating moth orchid virus-free seedlings. Background technique [0002] Phalaenopsis is a genus of Orchidaceae Phalaenopsis, native to subtropical rainforest areas, and is an epiphytic orchid. The thick white aerial roots of Phalaenopsis are exposed around the leaves. In addition to absorbing nutrients in the air, they also have growth and photosynthesis. In the Spring Festival, the Phalaenopsis plant has long flower stalks from the leaf axils and blooms like butterflies flying. It is favored by flower lovers and is known as the "Queen of the Orchid". [0003] Phalaenopsis is a single-stem aerial orchid with very little side branch development, so it is difficult to carry out division propagation routinely, and rarely forms seeds, and seed development is also relatively difficult. Basically, it is more difficult to germinate...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 郑秋桦郑翼泽许启晓陈曼丽贝淑琴吴志鸿
Owner SHAOGUAN COLLEGE
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