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Method for rapidly obtaining virus-free garlic

A garlic, fast technology, applied in the field of rapid acquisition of virus-free garlic, can solve the problems of restricting large-scale production and sustainable and healthy development of garlic, no virus-free research on garlic shoot tip plants, and degeneration of varieties, so as to improve the budding shoot tip. number and bud induction rate, providing economic value, and high survival rate

Inactive Publication Date: 2017-10-24
CHENGDU ACAD OF AGRI & FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Garlic is a vegetative plant, that is, it relies on bulbs for reproduction. During the reproduction process, viruses will accumulate year by year through garlic seeds and be passed on from generation to generation, resulting in serious degradation of species and weak garlic heads, which seriously restricts the large-scale production and sustainable and healthy development of garlic.
[0004] However, there is no series of virus-free research on garlic shoot tip induction, subculture, bulb culture, domesticated plants, etc.

Method used

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  • Method for rapidly obtaining virus-free garlic
  • Method for rapidly obtaining virus-free garlic

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Embodiment 1 shoot tip induction culture

[0027] Taking Yundingzao, Ershuizao, Pengxianzao and Wenjiang Hongqixing (referred to as Wenjiang Garlic) as the research objects, the garlic heads were heat-treated at 30°C for 30 days, then the stem tips were peeled off, and the stem tips of 0.1-0.2mm were respectively selected and placed on the stems. In the pointed induction medium, the culture temperature is 25° C., the light intensity is 2000 lx, and the light is 10-12 hours per day for 30-35 days.

[0028] The screening process of Yundingzao shoot tip induction medium:

[0029] (1) The shoot tip induction medium of Yundingzao is based on B5+BA 2-3.5mg / L+KT 0.3-0.6mg / L+NAA0.05-0.15mg / L as the screening basis, in which the BA content is 0.25mg / L The concentration gradient of L was changed, KT was changed according to the concentration gradient of 0.1mg / L, and NAA was changed according to the concentration gradient of 0.025mg / L. Orthogonal experiments were carried out, and...

Embodiment 2

[0041] Embodiment 2 Subculture

[0042] 1. Shoot tip subculture

[0043] The shoot tip culture obtained in Example 1 is subcultured to obtain test-tube plantlets. If the culture medium components and contents are different during the culture process, the shoot tip culture subculture can produce shoots, roots, and callus in an irregular manner, and some only Turn green, or even die, so the composition and content of the subculture medium are very important. The medium is based on MS or B5 medium, and also includes the following components: BA, KT and NAA. Change the content of BA, KT and NAA , screened out the following medium, and calculated its multiplication factor, see Table 2 for details.

[0044]G2(B5+BA 3.5mg / L+KT 0.5mg / L+NAA 0.1mg / L) and G21(MS+KT1mg / L+NAA 0.5mg / L) are suitable for the subculture of Yundingzao; G8(B5+BA 3mg / L+KT0.1mg / L+NAA 0.1mg / L) and G3 (B5+BA 4mg / L+KT0.5mg / L+NAA 0.1mg / L) are suitable for the subculture of Dishuizao; G18 (MS +BA 2mg / L+NAA 0.01mg / L)...

Embodiment 3

[0051] Embodiment 3 induces test tube bulb

[0052] The tissue culture seedling propagation lines obtained by subculture were placed in the bulb induction medium, and the culture temperature was 25°C, the light intensity was 2000lx, and the light was 12h per day and cultivated for 65 days; wherein, the bulb induction medium was MS medium The basal medium also includes the following components: BA 0.5-1.5mg / L, KT0-0.5mg / L and NAA 0.1-1.5mg / L; among them, the content of BA is changed according to the concentration gradient of 0.1mg / L, and the content of KT is changed according to the concentration gradient of 0.05 The concentration gradient of mg / L changes, NAA changes according to the concentration gradient of 0.1mg / L, carries out the orthogonal experiment, draws the bulb induction medium that is beneficial to 4 varieties to be MS+BA 1mg / L+NAA 1.5mg / L and The induction rates of MS+NAA 0.1mg / L+KT 0.5mg / L were 76.5% and 73.9% respectively.

[0053] The bulbs of Ershuizao, Yundin...

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Abstract

The invention discloses a method for rapidly obtaining virus-free garlic. The method comprises the following steps: (1) stem apex induced culture; (2) rachis culture: a rachis culture medium contains B5, 1.5-2.0 mg / L of BA and 0.05-0.15 mg / L of NAA; (3) induction of test tube bulbs: an MS medium is taken as a basic medium, and 1 mg / L of BA, 0-0.5 mg / L of KT and 0.1-1.5 mg / L of NAA are also contained; (4) domestication of plants with the test tube bulbs; (5) virus detection. According to the method, heat treatment stem apex culture and rachis subculture are combined, and propagation of virus-free plants can be accelerated; the virus-free plants are planted in fields after induction of the test tube bulbs, the survival rate of the virus-free plants is 89%, and the removal rate of four viruses, namely, OYDV, LYSV, GCLV and SLV, is 30.3%.

Description

technical field [0001] The invention belongs to the technical field of garlic detoxification, in particular to a method for quickly obtaining detoxification garlic. Background technique [0002] Garlic has a pungent and warm smell, is rich in protein, fat, sugar, calcium, iron and vitamins A, B, C and other nutrients, and has high medicinal value. Toxic gas" effect. Garlic is also a commonly used seasoning. When cooking melons, vegetables, fish, and meat, add a few cloves, which is fragrant and delicious. [0003] Garlic is a vegetative plant, that is, it relies on bulbs for reproduction. During the reproduction process, viruses will accumulate year by year through garlic seeds and be passed on from generation to generation, resulting in serious degradation of species and weak garlic heads, which seriously restricts the large-scale production and sustainable and healthy development of garlic. . [0004] However, there is no series of virus-free research on garlic shoot ti...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008A01H4/002A01G24/28A01G24/15A01G24/10
Inventor 帅正彬郭江洪杨斌潘少坤
Owner CHENGDU ACAD OF AGRI & FORESTRY SCI
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