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Comprehensive detoxification method of lily bulbs

A comprehensive and lily-based technology, applied in the field of comprehensive detoxification of lily bulbs, can solve problems such as genetic variation and difficult to understand the detoxification situation, and achieve the effects of high plant survival rate, shortened cultivation period, and reduced virus cross-infection

Inactive Publication Date: 2016-06-01
SONGTAO HONGFA MEAT FOOD CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The problem is: cut out very small shoot apex meristems and culture them on MS basic medium supplemented with 6-BAl. Callus and adventitious buds, but adventitious buds differentiated from callus are prone to genetic variation; and the virus has not been further detected and monitored, and it is difficult to understand the virus-free situation

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0028] Embodiment 1: lily bulb comprehensive detoxification method, comprises the following steps:

[0029] A, stripping of explant growth point: choose high-quality bulbs, soak 30 minutes in 600 times solution of carbendazim with a concentration of 20%; mix the sterilized peat with water so that its water content is 46%, Standby; then plant the bulbs soaked in carbendazim in the wet peat, plant 20 bulbs in each box, pour root water, and then sprinkle the carbendazim solution soaked in the bulbs on the surface; press The planting method of cut flowers is managed normally, and the moisture content of peat is kept at 46%. Under the conditions of temperature at 21°C, humidity at 70%, light intensity at 1600Lx, and light time at 8h / d, after 6 to 7 days, heat treatment is carried out when the buds grow to 6cm. Detox.

[0030] B. Detoxification by heat treatment: The temperature is controlled at 36°C, the humidity is 70%, the light intensity is 1600Lx, and the light time is 8h / d. A...

Embodiment 2

[0035] Embodiment 2: comprehensive detoxification method for lily bulbs, comprising the following steps:

[0036] A, stripping of explant growth point: select high-quality bulbs, soak 30 minutes with 30% carbendazim 600 times solution; mix the sterilized peat with water to make its water content 60%, Standby; then plant the bulbs soaked in carbendazim in the wet peat, plant 22 bulbs in each box, pour root water, and then sprinkle the carbendazim solution soaked in the bulbs on the surface; press The planting method of cut flowers is managed normally, and the water content of peat is kept at 60%. Under the conditions of temperature at 22°C, humidity at 76%, light intensity at 1800Lx, and light time at 10h / d, after 6 to 7 days, the buds grow to 6.6cm. Detoxification by heat treatment.

[0037] B. Detoxification by heat treatment: The temperature is controlled at 36°C, the humidity is 80%, the light intensity is 1600Lx, and the light time is 10h / d. After 7-16 days of cultivation...

Embodiment 3

[0043] Embodiment 3: lily bulb comprehensive detoxification method, comprises the following steps:

[0044] A, stripping of explant growth point: select high-quality bulbs, soak 30 minutes in 600 times solution of carbendazim with a concentration of 40%; mix the sterilized peat with water to make its water content 66%, Standby; then plant the bulbs soaked in carbendazim in the wet peat, plant 26 bulbs in each box, pour root water, and then sprinkle the carbendazim solution soaked in the bulbs on the surface; press The cut flower planting method is managed normally, and the moisture content of peat is 66%. Under the conditions of temperature 23°C, humidity 80%, light intensity 2000Lx, and light time 12h / d, after 6-7 days, heat treatment is carried out when the buds grow to 6cm. Detox.

[0045] B. Detoxification by heat treatment: The temperature is controlled at 38°C, the humidity is 86%, the light intensity is 1600Lx, and the light time is 12h / d. After 7-16 days of cultivatio...

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PUM

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Abstract

The invention provides a comprehensive detoxification method of lily bulbs. The method concretely comprises the following steps: 1, picking off explant growing points; 2, carrying out heat treatment detoxification; 3, cleaning and disinfecting explants; 4, and carrying out collection and induction culture on the growing pints; 5, carrying out detoxifying culture; and 6, detecting viruses. The method solves disadvantages in the prior art, solves the disadvantages of individual methods through comprehensively utilizing heat treatment detoxification, meristem culture detoxification and callus detoxification methods, and also has the advantages of effective reduction of the virus cross infection change, high detoxification rate, high survival rate, and effective shortening of the bulb culture period.

Description

technical field [0001] The invention belongs to a method for testing or treating seeds before sowing or planting, in particular to a method for detoxifying lily bulbs. Background technique [0002] Oriental lily is the largest and most beautiful category of lily hybrids. It can emit a pleasant fragrance, so it is also called perfume lily. It is deeply loved by people and is a large category of lily widely cultivated in the world. hybrid line. Lilies mainly reproduce by cuttings and balls, but long-term asexual reproduction causes viruses to accumulate in the body, causing various viral diseases, and seriously affecting the quality of bulbs and cut flowers. In recent years, with the number of lily introductions in my country and With the rapid increase of the planting area and the self-propagation of non-standard bulbs, viral diseases began to be prevalent in various lily planting areas in my country. The general incidence of viral diseases is 40-60%, and the second-generatio...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01C1/08
Inventor 杨长能
Owner SONGTAO HONGFA MEAT FOOD CO LTD
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