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Method for rapidly culturing shoot tips in vitro

A cultivation method and stem tip technology, which is applied in the field of in vitro rapid culture of strawberry stem tips, can solve problems such as strawberry infection with viruses, decline in yield and quality, and inability to meet the needs of production seedlings, and achieve high incidence of shoot tips and eliminate Effects of vitrification and rapid reproduction

Inactive Publication Date: 2012-04-25
上海航育种子有限公司
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AI Technical Summary

Problems solved by technology

For a long time, the propagation methods of strawberries have always been based on branch propagation and axillary bud cutting propagation. However, these traditional propagation methods are easy to infect strawberries with viruses, reducing their yield and quality, and cannot meet the needs of production seedlings.

Method used

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  • Method for rapidly culturing shoot tips in vitro

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Effect test

Embodiment 1

[0024] The composition of MS medium is as follows:

[0025]

[0026] Note: 1 / 2MS medium is prepared by halving the above-mentioned macroelements and trace elements, and keeping other components unchanged.

[0027] Strawberry stem tip rapid culture method in vitro, the specific steps are as follows:

[0028] Phase 1: Cleaning and disinfection of materials takes place. Select the stolons that germinated in the same year of strawberries, rinse them with detergent, and rinse them under running water for 2 hours. On the ultra-clean workbench, first disinfect with 75vol% ethanol solution for 1 minute, then disinfect with 0.1vol% Jieermin solution for 15 minutes, and finally disinfect with 0.1vol% mercuric chloride solution for 15 minutes, and rinse with sterile water three times , dry the water with sterile filter paper;

[0029] The second stage is the induction culture of sterile strains. Cut the sterilized strawberry stolons under a dissecting microscope into 0.3mm-long sh...

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Abstract

The invention provides a method for rapidly culturing shoot tips in vitro, which is characterized in that in the first step, selecting stolon of the strawberries sprouted at the same year, washing to be clean and disinfecting; in the second step, cutting 0.1-0.5 mm shoot tips of the disinfected and cleaned strawberries stolon under the anatomical lens, inoculating to a culture medium for germination culture, cutting the new sprouts after the 0.5-1.5 cm new sprouts are grown on the shoot tips, inoculating to an enrichment medium for inducing clustered shoots; in the third step, cutting the cluster buds to simple buds, inoculating to a rooting medium for rooting culture; transferring test tube plantlets to a substrate formed by mixing perlite and peat, watering and covering a plastic film for insulation and moisture retention, revealing the plastic film for gradually hardening, transferring the seedling to a plastic potted tray filled with pure peat, watering and covering the plastic film for insulation and moisture retention, and directly planting the seedling to a field. The invention has the advantages of high reproductive rate, rapid reproduction speed and high survival rate.

Description

technical field [0001] The invention relates to a rapid in vitro culture method of strawberry stem tips, which utilizes the stem tip growth points on the stolons pumped out in the past to be sterilized to induce clustered buds, and then obtains complete test-tube seedlings after culture, and belongs to the technical field of strawberry culture methods . Background technique [0002] Strawberry is a high-quality and high-grade fruit with rich nutrition and high economic value. For a long time, the propagation methods of strawberry have been based on ramet propagation and axillary bud cutting propagation. However, these traditional propagation methods are easy to infect strawberries with viruses, resulting in a decline in yield and quality, which cannot meet the needs of production seedlings. Contents of the invention [0003] The object of the invention is to provide a method for cultivating strawberries with high reproduction rate, fast reproduction speed and excellent re...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 张媁胡海峰
Owner 上海航育种子有限公司
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