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Ginger virus-free breeding method

A technology of ginger and detoxification seedlings, applied in the directions of horticultural methods, botanical equipment and methods, plant regeneration, etc., can solve problems such as poor detoxification effect of ginger, long period of original ginger seed, etc., and achieves improvement of detoxification rate of ginger, The effect of shortening the breeding cycle, high proliferation rate and detoxification rate

Inactive Publication Date: 2018-01-02
NEIJIANG NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a ginger detoxification breeding method, which can effectively solve the problem of poor detoxification effect of ginger in the prior art. long question

Method used

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  • Ginger virus-free breeding method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] The preparation of embodiment 1 ginger virus-free seedlings

[0034] 1. Effects of different heat treatments on the survival rate, proliferation rate and detoxification rate of ginger aseptic seedlings

[0035] Take the young shoots germinated from ginger rhizomes, peel off the outer leaf sheath, rinse with sterile water, then disinfect with 75% alcohol for 30-40s, rinse with sterile water for 3-4 times, and then use 0.1% HgCl 2 Disinfect for 8-10 minutes, continue to rinse with sterile water for 5-6 times, peel off the young shoots under a dissecting microscope, and take different lengths of stem tip growth points and insert them into a mixture containing 6-BA 0.5mg / L, KT 1.2mg / L , NAA 0.05mg / L and sucrose 16g / L MS medium, heat treatment temperature (A), treatment time (B) and shoot tip length (C) three factors, a total of 9 treatments, each treatment inoculated 8 bottles , each bottle received 4 shoot tips, and then cultured, first cultured in the dark at 25°C for 2 ...

Embodiment 2

[0055] The expansion propagation of embodiment 2 ginger virus-free seedlings

[0056] 1. Cut off the stems and leaves of the virus-free ginger seedlings, divide the bases into single buds, and re-inoculate them into new plants containing 6-BA0.5mg / L, KT 0.5mg / L, NAA 0.05mg / L and sucrose 15g / L. Cultivate in MS medium of L, cultivate at 23-26° C. for 40-45 days, and obtain a large number of virus-free ginger rooted shoots.

[0057] 2. After cutting the stems and leaves of the virus-free ginger seedlings, divide the bases into single buds, and re-inoculate them into new plants containing 6-BA 1mg / L, KT 1.5mg / L, NAA 0.15mg / L and sucrose 20g / L. Cultivate in MS medium, cultivate at 23-26° C. for 40-45 days, and obtain a large number of virus-free ginger rooted shoots.

[0058] 3. Cut off the stems and leaves of the virus-free ginger seedlings, divide the bases into single buds, and re-inoculate them into new plants containing 6-BA 0.5 mg / L, KT 1.2 mg / L, NAA 0.05 mg / L and sucrose 16...

Embodiment 3

[0060] Embodiment 3 transplanting domestication

[0061] Survival culture: plant the single-branch virus-free ginger seedlings with similar growth conditions obtained in Example 2 into 270 plastic pots, 2 plants per pot, and place them at 20-28°C for 2 weeks. This cultivation process only needs to be watered , do not need other operations such as fertilization; wherein, the cultivation substrate is a mixture of perlite, peat soil and biological bacterial fertilizer in a volume ratio of 1:3:0.5.

[0062] Among them, the biological bacterial fertilizer includes the following components by weight: 2 parts of compound bacteria, 40 parts of corn flour, 15 parts of bran, 3 parts of sucrose, 28 parts of fulvic acid, 6 parts of ginkgo biloba, 0.2 parts of ammonium molybdate, sulfuric acid 0.06 parts of zinc and 12 parts of water.

[0063] The composite bacteria include the following components in parts by weight: 1.2 parts of nitrogen-fixing bacteria, 1 part of phosphorus-solubilizin...

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Abstract

The invention discloses a ginger virus-free breeding method. The ginger virus-free breeding method comprises the following steps: (1) preparing ginger virus-free seedlings; (2) amplifying reproductionof the ginger virus-free seedlings; and (3) transplanting and domesticating, namely transplanting a large number of ginger virus-free seedlings obtained in the step (2) into a culture medium, and culturing for 1-2 weeks at the temperature of 20-28 DEG C, wherein the culture medium is a mixture obtained by mixing perlite, peat soil and a biological bacterial fertilizer in a volume ratio of (1-2):(3-5):(0.3-0.8), concentrations of nitrogen, phosphorus and potassium in earlier stage culture are 60-100mg / L, 20-40mg / L and 100-150mg / L respectively, concentrations of nitrogen, phosphorus and potassium in later stage are 200-220mg / L, 20-40mg / L and 200-250mg / L respectively, and a fertilizer is applied once a week. The ginger virus-free breeding method disclosed by the invention has the advantagesthat various parameters of the whole breeding process are studied, the optimized parameters are combined together, and virus-free percent, survival rate and yield of ginger can be effectively improvedwhile breeding period of the ginger is shortened.

Description

technical field [0001] The invention belongs to the technical field of ginger breeding, and in particular relates to a ginger-free breeding method. Background technique [0002] Ginger (Zingiber officinale Roscoe) is a perennial herb that has the effects of treating diarrhea, appetizing and invigorating the spleen, refreshing, anti-aging, treating internal diseases, and maintaining health. Its reproduction is mainly asexual reproduction through underground rhizomes. Since its growth and development have not had sexual generations, the virus is easy to accumulate in the body, resulting in shrinkage of ginger leaves, slow growth, reduced yield and quality degradation. Therefore, it is very necessary for modern ginger cultivation to remove the virus of ginger and keep the seed property of good varieties. But now the detoxification effect on ginger is not good, and the breeding of detoxified seedlings has not been truly industrialized like detoxified potatoes and strawberries. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00C05G3/00
Inventor 王辉肖小君何正兴杜慧胡艳黄作喜齐泽民李凤
Owner NEIJIANG NORMAL UNIV
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