Virus removing method for lily tissue culturing seedling by heat treatment

Abstract

A heat treating method for detoxicating the tissue-cultured seedlings of lily features that the lily bulb in germination state is treated at 35-50 deg.C and 100% air humidity for 20-40 days. Its advantages are high survival rate and high detoxicating effect.

Description

technical field [0001] The invention relates to plant tissue culture, in particular to a heat treatment detoxification method for lily tissue culture seedlings. Background technique [0002] Some important flowers and garden ornamental plants can be mass-produced with virus-free seedlings by tissue culture. A large number of flowers such as chrysanthemum, carnation, gladiolus, narcissus, tulip, lily, dahlia, and white crane are reproduced by asexual reproduction, and viruses are passed on and accumulated from generation to generation, and the harm is becoming more and more serious. The virus concentration will decrease and the activity will be inactivated after the infected plants are treated with high temperature. Heat treatment is generally carried out in hot air. , the treatment time ranges from a few days to dozens of days, and even some plants can be treated for dozens of days, and they are still alive. [0003] Lily flowers reproduce by asexual reproduction, and viru...

AI Technical Summary

Problems solved by technology

[0003] Lily flowers reproduce by asexual reproduction, and viruses are also passed and accumulated from generation to generation, but there is no detailed report on the heat treatment process of lily; mentioned in "Tissue Culture and Large-scale Production of Ornamental Flowers" edited by Xiong Li and Wu Lifang (P194) For bulbous flowers such as lilies, tulips, and hyacinths, heat treatment with dormant bulbs can reduce the virus content in the growth points of the bulbs; but there is no mention of how to heat treat lilies, how to ensure a higher survival rate and better The detoxification effect is the key to heat treatment