Virus removing method for lily tissue culturing seedling by heat treatment

A lily combination and lily technology, applied in the field of plant tissue culture, can solve the problems of reducing the virus content in the growing point of seed bulbs, the heat treatment process of lily is not reported in detail, and the heat treatment is not mentioned, etc., and achieves bright color, good detoxification effect, shortening effect of time

Inactive Publication Date: 2007-01-31
潘利军
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Problems solved by technology

[0003] Lily flowers reproduce by asexual reproduction, and viruses are also passed and accumulated from generation to generation, but there is no detailed report on the heat treatment process of lily; mentioned in "Tissue Culture and Large-scale Production of Ornamental Flowers" edited by Xiong Li and Wu Lifang (P19

Method used

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Example

[0012] The method of embodiment 1 lily tissue culture seedling heat treatment detoxification:

[0013] 1) Put 1600 bulbs of the first generation imported from the Netherlands in a germinating state in an incubator and perform the following heat treatments: control the air humidity in the incubator to 100%, and the treatment time is 30 days, and observe its survival rate and detoxification effect;

[0014] temperature(℃)

[0015] It can be seen from the above table that when the temperature gradually rises within the range of 35-55°C, the survival rate gradually decreases, and when the temperature reaches 50°C, the survival rate is below 50%; while the virus-free rate gradually increases, When the temperature reaches 40°C, the virus-free rate is 76.5%. Considering the above two factors, the better treatment temperature is 40-45°C.

[0016] From actual experience, it is known that the heat treatment time of the sprouting lily is related to the variety of the lily and t...

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PUM

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Abstract

A heat treating method for detoxicating the tissue-cultured seedlings of lily features that the lily bulb in germination state is treated at 35-50 deg.C and 100% air humidity for 20-40 days. Its advantages are high survival rate and high detoxicating effect.

Description

technical field [0001] The invention relates to plant tissue culture, in particular to a heat treatment detoxification method for lily tissue culture seedlings. Background technique [0002] Some important flowers and garden ornamental plants can be mass-produced with virus-free seedlings by tissue culture. A large number of flowers such as chrysanthemum, carnation, gladiolus, narcissus, tulip, lily, dahlia, and white crane are reproduced by asexual reproduction, and viruses are passed on and accumulated from generation to generation, and the harm is becoming more and more serious. The virus concentration will decrease and the activity will be inactivated after the infected plants are treated with high temperature. Heat treatment is generally carried out in hot air. , the treatment time ranges from a few days to dozens of days, and even some plants can be treated for dozens of days, and they are still alive. [0003] Lily flowers reproduce by asexual reproduction, and viru...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 潘利军
Owner 潘利军
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