Detoxification method of passion fruit

A technology of passion fruit and virus-free seedlings, which is applied in the field of economic crop cultivation, can solve problems such as difficulty in obtaining virus-free effects and distinctions, and achieve the effects of early fruit, high survival rate, and fast growth

Inactive Publication Date: 2017-01-04
韦中定
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The above three disclosed patents obtain virus-free seedlings by disinfecting the explanted organs of passion fruit and culturing them in vitro. The steps are simple, but it is difficult to distinguish whether they are virus-carrying plants when obtaining explants. It is difficult to obtain the ideal detoxification effect in point detoxification. Therefore, how to sterilize the passion fruit seedlings from various aspects, so as to obtain the detoxification seedlings of passion fruit, is worthy of further study

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] (1) Seed heat treatment: Take out the fully developed seeds from the passion fruit fruit, wrap them in gauze cloth, soak them in wet water at about 40°C for 6 minutes, then put them in hot water at 50°C for 55 minutes, and absorb the water after taking them out .

[0025] (2) Seed disinfection: Under aseptic conditions, soak the heat-treated seeds in 70% alcohol solution for 3-10 minutes, then put them in 8% bleaching powder clarification solution or 0.1% mercury liter for 5-10 minutes, and then use sterile Rinse with bacterial water 3 to 5 times.

[0026] (3) Rootstock culture: Peel off the seed coat, inoculate on the rootstock medium, inoculate 2 to 3 seeds in each test tube, germinate and cultivate in the dark at a constant temperature of 27±2°C, and wait for the seedlings to grow for 13 to 14 days. The rootstocks are grafted and cultured under scattered light indoors for 1-2 tons before grafting; the rootstock culture medium is composed of: MS inorganic salts + 3% ...

Embodiment 2

[0035] (1) Seed heat treatment: Take out the fully developed seeds from the passion fruit fruit, wrap them in gauze cloth, soak them in wet water at about 41°C for 5 minutes, then put them in hot water at 51°C for 55 minutes, and absorb the water after taking them out .

[0036] (2) Seed disinfection: Under aseptic conditions, soak the heat-treated seeds in 70% alcohol solution for 3-10 minutes, then put them in 8% bleaching powder clarification solution or 0.1% mercury liter for 5-10 minutes, and then use sterile Rinse with bacterial water 3 to 5 times.

[0037] (3) Rootstock culture: Peel off the seed coat, inoculate on the rootstock medium, inoculate 2 to 3 seeds in each test tube, germinate and cultivate in the dark at a constant temperature of 27±2°C, and wait for the seedlings to grow for 13 to 14 days. The rootstocks are grafted and cultured under scattered light indoors for 1-2 tons before grafting; the rootstock culture medium is composed of: MS inorganic salts + 3% ...

Embodiment 3

[0046] (1) Seed heat treatment: Take out the fully developed seeds from the passion fruit fruit, wrap them in gauze cloth, soak them in wet water at about 45°C for 6 minutes, then put them in hot water at 55°C for 55 minutes, and absorb the water after taking them out .

[0047] (2) Seed disinfection: Under aseptic conditions, soak the heat-treated seeds in 70% alcohol solution for 3-10 minutes, then put them in 8% bleaching powder clarification solution or 0.1% mercury liter for 5-10 minutes, and then use sterile Rinse with bacterial water 3 to 5 times.

[0048] (3) Rootstock culture: Peel off the seed coat, inoculate on the rootstock medium, inoculate 2 to 3 seeds in each test tube, germinate and cultivate in the dark at a constant temperature of 27±2°C, and wait for the seedlings to grow for 13 to 14 days. The rootstocks are grafted and cultured under scattered light indoors for 1-2 tons before grafting; the rootstock culture medium is composed of: MS inorganic salts + 3% ...

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PUM

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Abstract

The invention discloses a detoxification method of passion fruit. The method comprises the following steps: 1, carrying out heat treatment on seeds; 2, carrying out seed disinfection; 3, performing rootstock cultivation; 4, disinfecting stem tips; 5, carrying out heat treatment on the stem tips; 6, carrying out scion breeding; 7, grafting the stem tips; 8, performing rooting culture; 9, transplanting grafting seedlings; 10, carrying out PCR detection. By adopting the method disclosed by the invention, CMV and PWV of the passion fruit can be completely removed to solve the problem of difficulty in detoxification of passion fruit seedlings in the prior art, after 2-year experiments, the yield increase of the passion fruit reaches 50%, thus the detoxification method of passion fruit is worthy of being applied and popularized in practical production.

Description

technical field [0001] The invention relates to a technique for cultivating economic crops, in particular to a method for detoxifying passion fruit. Background technique [0002] Passion fruit (Passiflora edulis Sims), scientific name Passiflora, Passiflora, Passiflora genus, is a perennial evergreen vine berry fruit tree in the tropics and subtropics. Currently, passion fruit diseases and insect pests are serious, manifested in: root rot after planting, Adult plants are susceptible to infestation by diseases and insect pests, resulting in severe yield reduction. According to analysis, this is related to the virus carried by passion fruit seedlings. Passion fruit mosaic virus CMV and lignification virus PWV are the two most important viruses that harm passion fruit seedlings. The traditional detoxification method of passion fruit is: spraying the stems and leaves with medicament virus spirit, yellow sweet and sour vinegar, etc., the detoxification is not complete, and the p...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 韦中定
Owner 韦中定
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