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Phalaenopsis virus elimination and rapid propagation technique

A phalaenopsis technology, applied in the field of phalaenopsis seedling breeding, can solve the problems of poor plant growth, affecting ornamental value and economic value, and the survival rate is not higher than 30%.

Active Publication Date: 2017-03-08
SHANDONG BOHUA HIGHLY EFFICIENT ECOLOGICAL & AGRI TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Susceptible plants mainly cause symptoms such as mosaic, necrosis, deformity, and petal deformation, resulting in poor plant growth, small and few flowers, and shortened flowering period, which seriously affects its ornamental value and economic value.
And the domestic survival rate is not higher than 30% in the research about Phalaenopsis detoxification, and the detoxification rate is not higher than 80%. Therefore, it is of great practical significance to study a kind of method with high survival rate, good detoxification effect and easy operation.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Described Phalaenopsis detoxification and rapid propagation technology, concrete steps are as follows:

[0039] First, choose Phalaenopsis flowering strains that grow robustly, bloom 3-5 flowers, and carry the virus after testing, cut the pedicel section 3cm / section, and each section has a full axillary bud. 75% alcohol surface disinfection for 30s, 0.1% HgCl 2 Shock disinfection for 8 minutes (until there are no bubbles on the surface of the explant after shaking), rinse with sterile water for 3 times, peel off the axillary buds and leaves with pointed forceps, and then add 0.1% HgCl 2 Disinfect by shaking for 3 minutes, rinse 5 times with sterile water, and remove the affected area by HgCl 2 The poisoned ends were inoculated on the induction medium for induction culture.

[0040] Second, the clustered buds of the leaves are spread, and the ramets are connected to the medium for promoting shoot tip elongation, and after cultivating for 45 days, the third leaf is matu...

Embodiment 2

[0048] Described Phalaenopsis detoxification and rapid propagation technology, concrete steps are as follows:

[0049] First, choose a Phalaenopsis flowering plant that grows robustly, blooms 3-5 flowers, and carries the virus after detection, cuts the pedicel section 4cm / section, and each section has a full axillary bud. 75% alcohol surface disinfection for 30s, 0.1% HgCl 2 Shock disinfection for 9 minutes (until there are no air bubbles on the surface of the explant), rinse with sterile water for 3 times, peel off the axillary buds and leaves with pointed tweezers, and then add 0.1% HgCl 2 Disinfect by shaking for 2 minutes, rinse 5 times with sterile water, and remove the infected HgCl 2 The poisoned ends were inoculated on the induction medium to induce clustered buds.

[0050] Second, the clustered buds of the leaves are spread, and the ramets are connected to the medium for promoting shoot tip elongation, and after cultivating for 50 days, the third leaf is mature. Pr...

Embodiment 3

[0058] Described Phalaenopsis detoxification and rapid propagation technology, concrete steps are as follows:

[0059] First, select Phalaenopsis flowering strains that grow robustly, bloom 3-5 flowers, and carry the virus after detection, cut the pedicel section 5cm / section, and each section has 2 full axillary buds. 75% alcohol surface disinfection for 30s, 0.1% HgCl 2 Shake and disinfect for 10 minutes (until there are no bubbles on the surface of the explant after shaking), rinse with sterile water 3 times, peel off the axillary buds and leaves with pointed forceps, and then add 0.1% HgCl 2 Disinfect by shaking for 3 minutes, rinse 5 times with sterile water, and remove the affected area by HgCl 2 The poisoned ends were inoculated on the induction medium to induce clustered buds.

[0060] Second, the clustered buds of the leaves are spread, and the ramets are connected to the medium for promoting shoot tip elongation, and after cultivating for 50 days, the third leaf is ...

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Abstract

The invention relates to the technical field of phalaenopsis seedling propagation, in particular to a phalaenopsis virus elimination and rapid propagation method. By exploring of primary culture, stem tip elongation culture, virus elimination culture, seedling strengthening and rooting culture, a rapid propagation approach and a method for secondary phalaenopsis virus elimination on the basis of combination of a physical method and chemical agents. Innovatively, a stem tip elongation culture method and a method for increasing survival rate and virus elimination rate by combination of growing point cutting size and the chemical agents and secondary virus elimination are developed, and a phalaenopsis virus elimination and rapid propagation approach is established. The method has advantages of high survival rate and virus elimination rate, the survival rate is up to 80.13%, the virus elimination rate is up to 92.67%, and remarkable technical effects and promising promotion prospect are achieved.

Description

[0001] (1) Technical field: [0002] The invention relates to the technical field of phalaenopsis seedling breeding, in particular to a virus-free and rapid propagation method for phalaenopsis. [0003] (two) background technology: [0004] Phalaenopsis spp is a tropical or subtropical aerial orchid, also known as Phalaenopsis. Because of its graceful posture, bright color and long-lasting flowering period, it is known as the "Queen of Orchids". It is an important ornamental flower for indoor greening and beautification. There is a large demand for it in the foreign market. The viral disease of Phalaenopsis is a major problem that plagues the orchid world today. [0005] Currently, more than 25 viruses have been isolated from Phalaenopsis, among which Cymbidium mosaic virus (CyMV) and Odontoglossum ringspot virus (ORSV) are the most harmful and widely distributed. Infected plants mainly cause symptoms such as mosaic, necrosis, deformity, and petal deformation, resulting in po...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/008
Inventor 赵学彩陈晋銮赵红霞胡东关李媛媛
Owner SHANDONG BOHUA HIGHLY EFFICIENT ECOLOGICAL & AGRI TECH CO LTD
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